| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,407,293 | T→A | 6.6% | intergenic (+27/‑82) | accC → / → yhdT | biotin carboxylase/DUF997 domain‑containing protein YhdT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,407,293 | 0 | T | A | 6.6% | 136.4 / 2.1 | 56 | intergenic (+27/‑82) | accC/yhdT | biotin carboxylase/DUF997 domain‑containing protein YhdT |
| Reads supporting (aligned to +/- strand): ref base T (29/23); new base A (2/2); total (31/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-01 | |||||||||||
ACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTT > NC_000913/3407151‑3407435 | aCGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAt > 1:859720/1‑143 (MQ=255) aaaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAACACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGc > 2:676526/1‑143 (MQ=255) aaaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAGGGGCGGGTTTGCCGGc > 1:248054/1‑143 (MQ=255) aaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGcc < 1:876612/143‑1 (MQ=255) aCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtt > 1:663537/1‑143 (MQ=255) aCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCtt < 1:265860/143‑1 (MQ=255) aCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCAGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCtt < 1:621174/143‑1 (MQ=255) aaCGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGACAAACAACTCGGTCTTCAGGAAAAGTAAGACTGCTAAAGCGGCAAAAGGCCGGATTGTCCGGCCtctgt > 2:69321/1‑139 (MQ=255) cGTTGATCTGCAGATCCGGATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAATAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTtta < 2:612107/143‑1 (MQ=255) cGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCGACAAGCAGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTtta > 1:124911/1‑143 (MQ=255) tGAGCTGAAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTttatta < 2:882940/143‑1 (MQ=255) tGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTggg > 2:827587/1‑143 (MQ=255) gcGCATCATGAACTACTAGAACTTCCAGCATGGTTGCACTAGCACGTATGAGTTTGTGACAGGTTTGGGGGTTCAGGAAAAATAAGACTGCTAAATCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGAc < 2:660536/142‑1 (MQ=255) gagaACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTACTACTGGGGATCGACAACCCCCATACGGCAc > 2:635555/1‑143 (MQ=255) aCTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATc > 1:89951/1‑143 (MQ=255) ttCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATccc > 1:391195/1‑143 (MQ=255) ttCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATccc > 2:240067/1‑143 (MQ=255) tCCAGCATGGTGGCACTAACATACACTGTCTGGAGAAAAAGCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTGAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGGACAATcccc < 2:336659/143‑1 (MQ=255) tCCAGCATGCTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATcccc < 1:526928/143‑1 (MQ=255) aGCATGGTGGCACTAACATCCACTCTCTGGAGAAAACACTCGGTCTTCAGGAAAACTAAGACTGCTAAACCGTCAAAAGGCCGGCTTCTCCGGCCTTTTCTATTACTGGGGATCGACAACCCCCATCAGGTACAATCACCGCt > 2:76672/1‑143 (MQ=255) cATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCttt < 2:174219/143‑1 (MQ=255) tggCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCCAAAGGCCGGATTTTCCGGCCTTTCTTATTACTAAGGATCGACAACCCCCACACCGTACAATCCCCGCTCACTTCa > 2:38957/1‑143 (MQ=255) aCTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCa > 1:682058/1‑143 (MQ=255) aTCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCCTAAGGTCCAATCCCCGCTTTCTTCACCCATCAggg > 2:244813/1‑143 (MQ=255) aTCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAggg > 1:781779/1‑143 (MQ=255) tCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGa < 2:859720/143‑1 (MQ=255) tCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCACATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGa > 1:683025/1‑143 (MQ=255) tCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAATTTCCGGCCTTTTTTATTACTGGGGATCGCCAACCACCCTAAGGTACAATGCCCGCTTTCTTCACCCACCAGGGa > 2:139549/1‑143 (MQ=255) cTATCTGGAGAAAAAACTCGGTCTTCAGAAAAACTAAGGATGCTGAAGTTGCAAGAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGCCaaa > 1:372753/1‑143 (MQ=255) cTGGAGAAAAAACTCGGTCTTCAGGAAAAAGAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAAt < 2:345505/143‑1 (MQ=255) tGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAACTg > 1:138070/1‑143 (MQ=255) gggaAAGAACTGGGTCTTCAGGAACACTAAGACTGCTGAACCGTCACAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTAGAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGa < 1:377236/141‑1 (MQ=255) aaaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATCAGGTACAATCCCCGCTTTCTTCACCCATCACGGACAAAAAACGGaccc > 2:479760/1‑141 (MQ=255) agacgcGGTCTTCAGGAACAAGAAGCCGGCTAAAGCGGCAAAAGGCCGGATGTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACAGTCg < 2:769753/138‑1 (MQ=255) aCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGtt < 1:827587/143‑1 (MQ=255) ttgttCTTCAGGAAAATTAAGACTGCTAAAGCGTCAAAAGGCCGGATGTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGtttt < 1:267826/139‑1 (MQ=255) tCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACACTCCCCGCTTTCTTCACCCATCAGGGACAAACCATGGACCCTCGtttt > 1:621720/1‑143 (MQ=255) tCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGtttt > 1:463837/1‑143 (MQ=255) cGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAGAAAAAGGACACACGctttt > 1:362126/1‑142 (MQ=255) cTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCa < 1:560995/143‑1 (MQ=255) ttCAGGAAAACTAAGCCTGCTAAGGCGTCAAAAGGCCGCATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAg < 2:875203/143‑1 (MQ=255) ctCAAGAAAAATAGGACTGCTAAAGCGTCAAAAGGCCGCATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCAGAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAg < 2:608595/142‑1 (MQ=255) tCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAgg < 1:185738/143‑1 (MQ=255) aGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCCTAAGGTCCAATCCCCGCTCTCTTCGCCCACCAGGGCCAACACACGGACCCCCGTTTCGTCCCGGcc > 2:261317/1‑143 (MQ=255) ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGAACCTCGTTTTGTTCAGGccc > 1:107458/1‑143 (MQ=255) aaaaaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCAt > 1:861665/1‑143 (MQ=255) aaaTAAGACGGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATaa < 1:300931/143‑1 (MQ=255) aaGACTGCTCACGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAagag < 2:559418/143‑1 (MQ=255) aCTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGCCAAAAAATGGCCACTCGTTTTGTTCAGTCCCATAAACGGgcg > 2:258261/1‑143 (MQ=255) ttAAAGCGTCAAATGGCCGGTTTTTCCGGTCTTTTTTATTACTGGGGATCGACAACCCCCATGAGGTACAATCCCCGCTTTCTTCCCCCATCAGGGACAAAGAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgct < 1:783764/142‑1 (MQ=255) aaaGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgg > 1:96808/1‑143 (MQ=255) aaaaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAACAATGGACACTCGTTTTGTTCCGGCTCATGAAGAGGCgcgctgagcgctgg > 1:319838/2‑143 (MQ=255) aaaaGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACACCCCCCAGAAGGTACAATCCCCGCTTTCTGCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGcgctgggcgctggg < 2:598807/143‑1 (MQ=255) gCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGa < 1:729566/143‑1 (MQ=255) ggATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACCCTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGAGCTCAcac > 1:818647/1‑141 (MQ=255) ggATTTTCCGGCCTTTTGTATTAGTGGGGCTACATAAGGTCCAGGAGATACAATCCACGCATTCTTCAACCCTTAGGGACAAAAAATGGACACTCGTTTTGATCAGGCCCATAAATAGGCGCGATGGGCGCTGGGGCTgcacc > 2:271281/1‑139 (MQ=255) gATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACTAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCt < 1:676526/143‑1 (MQ=255) ttttCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCGGGGGCTGACCCttt > 1:455750/1‑143 (MQ=255) | ACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTT > NC_000913/3407151‑3407435 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |