| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 600,233 | G→A | 12.7% | G507D (GGC→GAC) | cusA → | copper/silver export system RND permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 600,233 | 0 | G | A | 12.7% | 66.0 / 2.5 | 32 | G507D (GGC→GAC) | cusA | copper/silver export system RND permease |
| Reads supporting (aligned to +/- strand): ref base G (17/10); new base A (2/2); total (19/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01 | |||||||||||
TCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCT > NC_000913/600091‑600367 | tggggtATCCGGATCTTCAGGCTGGAAGGGCGGGAAGGGCGTCTGTTGGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTgg < 2:835216/138‑1 (MQ=255) ttCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGGGGCAACATTCCGCCggg > 2:726791/1‑142 (MQ=255) tGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGt > 1:564748/1‑143 (MQ=255) aGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGATCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGAACCTGATGGCCTACTGGATCCGTGGCAAAAATCCGCCGGAAACCAGTAAcc > 2:596707/1‑143 (MQ=255) gggCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGGGGGTGCGGCGCTGCTGGCGATCGTAGTGCTCCCGATCCTGCTGGGCTACTGGATCCGTGGCAAAATTCCCCCCGAAAGCAGTAAccc > 2:391080/1‑143 (MQ=255) gggCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAAccc < 1:657755/143‑1 (MQ=255) cAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGACAAAATTCCGCCGGAAAGCAGTAACCCGCt > 1:410501/1‑143 (MQ=255) cAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGAC‑AAATTCCGCCGGAAAGCAGTAACCCGCTc > 1:882629/1‑143 (MQ=255) tCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCtttt > 1:251645/1‑143 (MQ=255) gcgcGTTGGCGGTGACCAAATCGGATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCgt < 2:494680/140‑1 (MQ=255) cccGTTGGCGTTCACCAAAACGTATGTGTTTTGTTCTGCTGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCgtg < 1:682552/143‑1 (MQ=255) cccGGTGGCGTGCACGAAGACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCCACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCgtg < 2:418912/143‑1 (MQ=255) ccGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCgtgt > 1:137414/1‑143 (MQ=255) cGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTGTTGCTTCGTGtt > 1:543633/1‑143 (MQ=255) tGGCGTTCACCAGAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGAGCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTGGTGATTCGTGGTTat > 1:502302/1‑143 (MQ=255) gCGTTCACCAAAACGGATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCAGTGGCAAAATTCCGCCGGAACGCAGTAACCCGCTCAACCGCTTCTTGAGTCGTGGTTatca > 2:868909/1‑143 (MQ=255) gTTCACCGAAGCGTGTGCGATGGCGGGTGCGGCGCTGCTGGCGGTCGTGGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTACCCCGCTCAATCGCTTTTTGATTCGTGTTTatcatc < 2:697025/143‑1 (MQ=255) ttGCGGTGGCGGGTGCGGCGCTGTTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGaaa < 2:125111/142‑1 (MQ=255) gATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAACATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCAACCGCTGTTTCTGAAAGTAc > 2:344593/1‑143 (MQ=255) gggCGGGTGCGGCGCTGCTGGCGAGCGTAGTGATCCCGATCCGGATGGGCTACTGGATCCGTGAC‑AAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGc < 2:882629/142‑1 (MQ=255) ggCGGGGGGGGCGCTGCTGGCGGTCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGACAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGc < 2:410501/143‑1 (MQ=255) tGCGGCGCTGCCGGCGATCGTGGTGACCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGc < 1:179360/143‑1 (MQ=255) ggcgCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGCGTTTATCATCCGCTGCTGCTGCACGCACTGCACTGGCCGa > 2:837372/1‑143 (MQ=255) gctgctGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTGTGATTCGTGTTTAGCATCCGCTGTTGCGGAAAGTACTGCACTGGCCGaaaa > 1:608840/1‑143 (MQ=255) cGATTGTGGTGTTGCCGCGCCTGTTGTGCTACTGGATCCGTGGCGACATTTCGCCGGAAAGCAGTAACCCGCGCGATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACgctg < 2:107576/143‑1 (MQ=255) aTCGTAGTGATCCCGATCCTGATGGGCTATTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTATTCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACgctgct < 2:307958/143‑1 (MQ=255) tAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCtggtg > 1:389539/1‑143 (MQ=255) tGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTggcg > 1:291364/1‑143 (MQ=255) ccTGAGGGGCGACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAACACCACGCGGTTGGTGGCGGCGCTTTCgg < 2:535779/143‑1 (MQ=255) cTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACgg > 1:686509/1‑143 (MQ=255) tACTGGATGCGTCTCAAATTTCCGCCGGAAAGCAGTATCCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGt < 1:543637/143‑1 (MQ=255) tACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGt > 1:17143/1‑143 (MQ=255) ggATCCGTGGCAAAATTCCGCCGAAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTAATGCACTGGCCGAAAACCACGCTGCTGGTCGCGGCGCTTTCGCTCATGACGGTtctc > 2:873159/1‑143 (MQ=255) ggtgcggggCAAAATTCCGCCGGATAGCAGTTAGCCGCTCAGTCGCGGTTTGATTCGTGTTTATCATCCGCTCTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTctct < 1:548235/136‑1 (MQ=255) | TCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCT > NC_000913/600091‑600367 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |