Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,147,304 G→C 8.4% R56P (CGG→CCG)  frwD → putative PTS enzyme IIB component FrwD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,147,3040GC 8.4% 126.6 / 3.4 44R56P (CGG→CCG) frwDputative PTS enzyme IIB component FrwD
Reads supporting (aligned to +/- strand):  ref base G (19/21);  new base C (2/2);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

GCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCT  >  NC_000913/4147172‑4147442
                                                                                                                                    |                                                                                                                                          
gcggggTGGCGCATACTTATACGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCCGGCGGAtatc                                                                                                                                  <  1:690357/143‑4 (MQ=255)
gcggcgTGGCGCATACTTGTATGGGGGCGGACCGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGtt                                                                                                                                  <  2:352882/143‑1 (MQ=255)
      tGGCGCATCCTTATTTTGCGGCGGAACGGCTGGAAAAGTTGCCCCTGTTAGTGATGTGGTGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTg                                                                                                                            <  1:403359/143‑1 (MQ=255)
        gcgcATACTTATATGGCGGCCGAACGGCATGAAAAGGTGTGCCTGTTAGAGAAGTGAGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGtt                                                                                                                          <  1:578878/143‑1 (MQ=255)
          gcATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATCTCCGCCGGGAGGACGATGCACTGTGGa                                                                                                                        >  1:233127/1‑143 (MQ=255)
              aCTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGAAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTAc                                                                                                                    >  1:485236/1‑143 (MQ=255)
               cttagttGGCTGCGGAACGGGTGGAAAAGTTGTGCCTGTTAGGGAAGTGGGGAGTCGGCATTGAACCCCAGGGCGCGCTGGGAACGGAGAATCGTGTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACg                                                                                                                   <  1:535415/137‑1 (MQ=255)
                ttATAGGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGGCGTGGGCAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACgg                                                                                                                  <  1:403966/143‑1 (MQ=255)
                 tataTGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGa                                                                                                                 <  2:27490/143‑1 (MQ=255)
                       gcggcgGAACGGCTGAAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGAGTACGGATATCGa                                                                                                           >  2:743408/1‑143 (MQ=255)
                        cggcggAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAg                                                                                                          <  2:729627/143‑1 (MQ=255)
                                  gCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGTAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATAGAGCTTGGCGGTg                                                                                                >  1:518246/1‑143 (MQ=255)
                                  gCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTg                                                                                                >  2:837789/1‑143 (MQ=255)
                                      gAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGa                                                                                            >  1:488040/1‑143 (MQ=255)
                                        aaaGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGc                                                                                          >  1:364063/1‑143 (MQ=255)
                                            ttGTGCCTGTTAGAGAAGTGGGGAGTAAGAACCCTAACGTATGGCTCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGAtt                                                                                      <  1:831086/143‑1 (MQ=255)
                                             tgtgCCCGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCCGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGTATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGAttt                                                                                     <  1:665338/143‑1 (MQ=255)
                                             cgtgCCTGTTAGAGAAGTGGGGAGTGAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTTTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGAttt                                                                                     <  1:107925/142‑1 (MQ=255)
                                               tgCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGAtgtgc                                                                                   >  2:680144/1‑142 (MQ=255)
                                               tgCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTCCCGAGCGGTTTGa                                                                                   >  2:81733/1‑143 (MQ=255)
                                                   tGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACAt                                                                               <  2:488040/143‑1 (MQ=255)
                                                     ttAGAGATGTGGGGTGTCAGCATTGAATCTCTGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTg                                                                             <  2:267485/143‑1 (MQ=255)
                                                              tGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTAtgt                                                                    >  1:451597/1‑143 (MQ=255)
                                                                  gAGTCAGCATTGAAACTCAGGGCGCGCTCGGACCGGATTAACGTTTAGCGGATGAGGATATCCGCCGGGCGGCTGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGCGCGATTTGAACCTTGCGCCCATGTGCaa                                                                >  2:271067/1‑143 (MQ=255)
                                                                  gAGTCAGCATCGAAGGTCAGGGCGCGCGGGGAACGGAGAATCGTTGAGCGGATGAGGAGATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCaa                                                                <  1:821357/143‑1 (MQ=255)
                                                                  gAGGCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAAGCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCaa                                                                <  2:206077/143‑1 (MQ=255)
                                                                        gCATTGCAACTCAGGGCGCGCTGGGAAGGGCGAATCGTTTAGCGGATGAGCACATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATgcagc                                                          <  1:60647/143‑1 (MQ=255)
                                                                             gAAACTCAGGGCTCGCTGTGGACGGATAATCGTCTAGCGGATGAGGATATCCGCCCGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTa                                                     <  1:134130/143‑1 (MQ=255)
                                                                             gAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTa                                                     >  1:409384/1‑143 (MQ=255)
                                                                              aaaCTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGACCCCACCACTGGCGCTGTGGGCAATGCAGCAACTAc                                                    >  2:616269/1‑143 (MQ=255)
                                                                                    aGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTc                                              <  1:761524/143‑1 (MQ=255)
                                                                                     gggCGCGCTGGGAACGGAGAATCGTGTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTcc                                             <  1:492978/143‑1 (MQ=255)
                                                                                          cgcTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGt                                        >  2:628508/1‑143 (MQ=255)
                                                                                           gcTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGAGATTTGAACATTGCCGGTAGGTGCAATGCAGCATCTACGCATTCCTGCGTg                                       >  1:92129/1‑143 (MQ=255)
                                                                                              gggAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGAGTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCgggggc                                    >  1:427779/1‑138 (MQ=255)
                                                                                               ggAACGGAGAATCGTTTAGGGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACACTGCCGCTGTGTGGAATGCAGCATCTACGCATTCCTGCGTGAGcc                                   <  2:189513/143‑1 (MQ=255)
                                                                                                 aaCGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCCGCCTCTACGCATTCCTGCGTGcgccgc                                 >  2:419053/1‑143 (MQ=255)
                                                                                                    ggAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGCGCTTGCCTGTGCCGAGCGAATTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTCCGTGAGCcgccgc                              >  2:330048/1‑143 (MQ=255)
                                                                                                        gaTCGTTGAGCTGATGAGGATATCCGCCGGGGGGATGTTGCTCTGTTGATGACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGt                          <  2:157485/142‑1 (MQ=255)
                                                                                                               tAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGCTGCCGAGCGATTTGTCCACTTTCAACAGTTCCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGAGAAATGAgc                   >  1:875445/1‑143 (MQ=255)
                                                                                                                    gATGAGGATATCCGCCGGGCGGATGTTGCGCTGTTGATTACGGGTATCCGGCTCCCGTCCGCTGATCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGt              <  1:641778/143‑1 (MQ=255)
                                                                                                                      gagcaacatCCGCCCGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTgc            >  2:320199/8‑143 (MQ=255)
                                                                                                                       gaggagATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTgcg           <  2:469861/143‑1 (MQ=255)
                                                                                                                           acatCCGCCCGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGGAATGAGCGCGGTGCGCaaa       >  1:309881/3‑143 (MQ=255)
                                                                                                                                cgccgGGCGGATGTTGCGCGGGTGATTACGGATAGCGAGCGTGCCGGTGCCGAGCGAGTTGAACATTGCCGCTACGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCt  <  2:636169/143‑1 (MQ=255)
                                                                                                                                cgccgGGCGGATGTGGCTCTGTTGATTACGGATATCGAGCTGGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCt  <  1:60659/143‑1 (MQ=255)
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GCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCT  >  NC_000913/4147172‑4147442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑