| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,196,196 | T→C | 100% | intergenic (‑97/+136) | thiC ← / ← rsd | phosphomethylpyrimidine synthase/regulator of sigma D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,196,196 | 0 | T | C | 100.0% | 143.7 / NA | 46 | intergenic (‑97/+136) | thiC/rsd | phosphomethylpyrimidine synthase/regulator of sigma D |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/29); total (17/29) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCA > NC_000913/4196063‑4196334 | cgcgTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGc < 2:756905/143‑1 (MQ=255) cgTTGTTCGCGGCGGGTCAGTTTCGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAg < 1:464308/143‑1 (MQ=255) cgTTGTTCGCGGCGGGGCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAg < 2:178729/143‑1 (MQ=255) tgttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGACGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCGTGCTCCCTTCGCAGGTa < 2:20627/143‑1 (MQ=255) gtccgcgGCGGGTCAGTGTTGCGCCTGGCATAGCTCATGCCGAAAAGTGAGGGCCGGGGCTCGCCAGACGACGGCTGAAGCAAGAGCCGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCCCCCTTCGCAGGTAt < 2:820746/140‑1 (MQ=255) tCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTTCTTCCTTCGCCGGTATTa > 1:649490/1‑143 (MQ=255) cggcggGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCCAGAGACGATCGCCGCAGGGGCGATGCCATAGCAGATTAACTCTTTCTCCCTTCGCAGGTCTTAGcc > 2:690273/1‑143 (MQ=255) cggcggGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTCTGAGcc > 2:48117/1‑143 (MQ=255) gcggGTCAGTTGTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCACACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTg < 2:435781/143‑1 (MQ=255) gTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACCCTTGCTCCCTTCGCCGCTATCAGCCTGATCAGGtt > 2:654591/1‑143 (MQ=255) ttttGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATGAACTCTTGCTCCCTTCGCAGGTATTAGCGGGATCAggggc > 1:24589/1‑140 (MQ=255) tgttgCTGACTCACCTCATCCCAATCACTTGCGGACTGGGCTTGTTAGACGACTGATGCAGCAAGACACGTTTGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCTTTCGCAGGTATTAGCCTGATCAGGTTCcgc < 2:414300/143‑1 (MQ=255) gttgCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAGCTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCcgcg < 2:793842/143‑1 (MQ=255) gttgCAGACAGAGCTCGTTCCAGAAAGTTAAGGACTTGGCGTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCcgcg < 2:364929/143‑1 (MQ=255) ttgCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCgg > 1:346156/1‑143 (MQ=255) gCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCCGac > 2:202522/1‑142 (MQ=255) cAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGTAATTTATACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATc < 2:181543/143‑1 (MQ=255) aCATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCg < 2:293245/143‑1 (MQ=255) agaGCTCATTCAGAGAGGTTAAGGGCGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCAGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGaa < 2:313911/141‑1 (MQ=255) aTAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGaa < 1:275021/143‑1 (MQ=255) tCATTCCAAAAAGTTAAGGACGTGGCGTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAAc < 2:730417/143‑1 (MQ=255) cATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACg < 2:855796/143‑1 (MQ=255) aaaaaGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGGCTCa > 2:737989/1‑143 (MQ=255) aGTTAAGCACGTGGCCTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCa < 1:47595/143‑1 (MQ=255) aGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTaa < 1:524409/143‑1 (MQ=255) aGGACGGGGCTTTCCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTaa < 1:538678/143‑1 (MQ=255) gACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGGGCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAgg > 1:373745/1‑143 (MQ=255) gACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAgg < 1:791700/143‑1 (MQ=255) gtagagGACGGATGAAGCAAGAGACGGTCGCTGCAGGGGCGAGGCGATAGCAGATGAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCACGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACaa < 2:554864/141‑1 (MQ=255) gacgacGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGaa < 1:801118/143‑1 (MQ=255) acgacgGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGAACTCCGTCAAGaaa < 2:357562/143‑1 (MQ=255) aTGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATCAACGGTCTCAGCCAGTTAAGGCACTCCGACAATAAATTAGccc > 1:16451/1‑143 (MQ=255) agagACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATg > 2:690950/1‑143 (MQ=255) gagaCGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCAGATCCCGACTAAACGGGCTCAGCCACGTCAGGCACCCCGCCAACACATTAGCCCAGCACATgg > 1:732389/1‑143 (MQ=255) gagaCGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTACCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATgg > 1:461963/1‑143 (MQ=255) agaCGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCGGCCCGTTAAGGCACTCCGGCAAGAAATTAGCCCCGCAAATggg > 2:359652/1‑143 (MQ=255) gggCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTAGTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAAGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCACTGAATCTAAAt > 2:687318/1‑143 (MQ=255) gcgatgcgatAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTa < 1:721739/143‑1 (MQ=255) gatgcgatAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGCAAATTAcg > 2:866307/1‑143 (MQ=255) atgcgttACCAGATTAACTCTTGCTCTCTTCGCAGGTATTAGCGTGATTAGGTTCCGCGGCTCCCGAGTAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTAcgc < 2:660768/143‑1 (MQ=255) cgatAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGtt < 2:778377/143‑1 (MQ=255) aGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACa < 1:705063/143‑1 (MQ=255) aGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACa < 1:813213/143‑1 (MQ=255) gCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGCAAATTACGCGTTAACAg < 1:866307/143‑1 (MQ=255) tctTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTc > 1:723776/1‑143 (MQ=255) ctTGCTCCTTTCGCAGGTAGTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACGCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCa < 2:30785/143‑1 (MQ=255) | CGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCA > NC_000913/4196063‑4196334 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |