| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,532,537 | G→C | 12.5% | A216G (GCC→GGC) | yjhP ← | KpLE2 phage‑like element; putative methyltransferase YjhP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,532,537 | 0 | G | C | 12.5% | 78.2 / 4.2 | 32 | A216G (GCC→GGC) | yjhP | KpLE2 phage‑like element; putative methyltransferase YjhP |
| Reads supporting (aligned to +/- strand): ref base G (11/16); new base C (2/2); total (14/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01 | |||||||||||
CCTCGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAAC > NC_000913/4532399‑4532665 | ccgcGATAGCTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGaa < 1:370726/140‑1 (MQ=255) cGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTc > 2:407586/1‑143 (MQ=255) atatTTCCAGCCAGAAATACTGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCCTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGCCGCCGAAGTCag < 1:842226/143‑2 (MQ=255) atatGTCCAGCCAGAACTACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCAt < 1:615896/143‑1 (MQ=255) tcttaccagcgtgAAGTACCGGGACGCTGTTGTTACCGGGCGCTGAGTGCGAACACGCCCCAGCCACAGCAGTCCCGCGCCTAGGTCACGTATCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGCCGCCGAAGTCagg < 2:571505/138‑3 (MQ=255) tCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCa < 1:418360/143‑1 (MQ=255) ggccagAACTATCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCGGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGa < 2:384724/142‑1 (MQ=255) gAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGCTTCGGCGCGCTGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGc > 2:817304/1‑143 (MQ=255) ctctACCGCGTACCTGGTGTTGGCGGCTGAGGCGTTCGAACACGCCCCACCCGATGCATTCCCTCGCTTACGTCCCGTAGCCTTGCGGCACGATGTTTAGTTCGGCCCTTACCTCGGCGGCGAAGTCATCGTCAGGATTCGCt < 2:294637/140‑1 (MQ=255) aTAGCGGGAACCGGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTc < 2:646243/143‑1 (MQ=255) aCCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTcca < 1:862636/143‑1 (MQ=255) cgcgcaCCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTccagc < 2:489217/138‑1 (MQ=255) cGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAGGCATCCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTccagc < 2:118823/143‑1 (MQ=255) cGGGAACCTGTTGTTACCGGGCGAGGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTccagc < 1:749626/143‑1 (MQ=255) cTGTTGTTACCGGGCGATGAGTGCGAACACCCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGACGCTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTCCCAGCCAgcggc > 1:535533/1‑143 (MQ=255) gTGCGAACCCGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCg < 1:303017/143‑1 (MQ=255) gTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTCGTTCGGCCCTGACTTCGGCGGCGAAGGCATCGTCAGGATTCGCCTCCAGCCAGCGGCGCCTGGTCAGACATTTTGCg > 2:176825/1‑143 (MQ=255) cgcgCCAGCCAAAGCATGCCCGGGCGGAGGTCACGGAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGGCTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATAc < 1:450281/143‑1 (MQ=255) ccccAGCCAAAGCATTCCCGCGCGAAGGTCACGTAGCGTTTCGGCGCGCTGTTTCGTTCGTCCCTGGGTTGGGACACGAACCCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCGTACCg > 1:878945/1‑143 (MQ=255) cgaGCCAAAGCTTTTCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCATCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGt < 2:170984/141‑1 (MQ=255) ccAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCCTCACACCCGt > 2:411212/1‑143 (MQ=255) ccAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTAAGGCCCTGACTCCGGCGCCGAAGTCATCGTCAGGCTTCGCTTCCAGCCAGCGGCGCCTGGTCAGCCATTTTGCGGCTTCATCCCGGt > 1:554242/1‑143 (MQ=255) ttCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCt > 1:301959/1‑143 (MQ=255) gtCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCt < 2:384334/142‑1 (MQ=255) ccGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGt > 1:515043/1‑143 (MQ=255) cggaGGTCACGGAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGTATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGcc < 1:477241/140‑1 (MQ=255) gTAGGTCACGTGGCGTTTCGGCGCGTTGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCa < 2:683139/143‑1 (MQ=255) gcgcGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAACTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCTAGCCTTCCTGGTCTGCCAGTACCATTTCTACCCCggcg > 1:688876/1‑140 (MQ=255) cGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCCGGGTCAGCCATTTTGCGGCTTCATCCCGGTCCCAGCCTTCCTGGTCTGCCCGTACCATTTCCACCCCGGCCTAGCCGAGCtcgtcg > 1:294852/1‑143 (MQ=255) cGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGAtcggcg > 1:807462/1‑143 (MQ=255) acgatGACTTCGCCGCCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATCCCGGTCCCCGCCTTCCCGGTCTGCCAGTACCATTTCCCCCACCTCATAGCCGCGATCCCCGaa > 2:70452/5‑143 (MQ=255) cccTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAAc > 1:532683/1‑143 (MQ=255) | CCTCGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAAC > NC_000913/4532399‑4532665 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |