| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 710,436 | G→A | 100% | H71Y (CAC→TAC) | fur ← | DNA‑binding transcriptional dual regulator Fur |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 710,436 | 0 | G | A | 98.6% | 92.7 / ‑6.6 | 35 | H71Y (CAC→TAC) | fur | DNA‑binding transcriptional dual regulator Fur |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (13/19); total (14/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTTGCGGCAATTTCACGCTGACGCGCTTCGATGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAA > NC_000913/710297‑710573 | tttGCGGCAACTTCACGCTGACGCGCTTCGATGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGTAGATCAGGTGATCGTGGTGATGTTGCTGTGCCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCg < 1:668645/143‑1 (MQ=255) ggCAATTTCACGCTGACGCGCTTCGATGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCCGCTGCGGCGATCCTGGGGATCTTGCTGCGTCAGTTCAAATACGGATTTACCCCCTACACAATTGTGGCGGGGGa > 2:714304/1‑143 (MQ=255) gCAATTTCACGCTGACGCGCTTCGATGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGAc > 1:452846/1‑143 (MQ=255) aTTTCACGCTGACGCGCTTCGATGGAATCATCACTAGAGTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATATTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGAt < 2:771997/143‑1 (MQ=255) tttCACGCTGACGCGCTTCGATGGAATCATCACTAAATTCGAGAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATa < 1:37555/143‑1 (MQ=255) ttCACGCGGACGCGCTTCGATGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATAc < 1:778878/143‑1 (MQ=255) gcgcTTCGATGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGAGAGGTTGCTGGGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCa > 2:715831/1‑143 (MQ=255) aTGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGtt < 2:507200/143‑1 (MQ=255) aTGGAATCATCACGAAATTCGACAACCTCGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGtt < 1:304183/143‑1 (MQ=255) ggAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATGTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCa < 1:665355/143‑1 (MQ=255) cTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGata < 1:198756/143‑1 (MQ=255) ccgtGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAATTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGa < 2:232192/140‑1 (MQ=255) cTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCACCTTCAAAATTGTAGAGGGTGACGATACCAGCGTCGTAAAACTGGTTAAAACCGCGATATGACGTaagcaaac > 2:3350/1‑136 (MQ=255) ccGCAGTCGAGGCAGATCAGGTGCTCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAAt < 1:749620/143‑1 (MQ=255) ctgTCGAGCTAGATCATGTGATCGTGGTGATGTTGCTGTGTCTGTTCAACTCAGGCTTTCCCGTCCAGCAAATTGTAGCGGGTGACGATACCAGCGTCGTCACACTCGTACAGTACGCGATATACCGTAGCCAGACCAATttc < 2:668717/141‑1 (MQ=255) gTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATttctt > 1:424563/1‑143 (MQ=255) tCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCGCATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGGTATACCGTCGCCAGGCCAATttcttc > 1:798157/1‑143 (MQ=255) tCGAGCCAGATCAGGTGCTCGTGGTGATGTTGCTGTGTGCGTTCAAATACGGATTTACCGCCTTTAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATttcttc < 1:495454/143‑1 (MQ=255) ggCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCAccc > 2:110766/1‑143 (MQ=255) tCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATGGATCAGACg > 1:389706/1‑143 (MQ=255) tggtgATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGttt < 1:866500/143‑1 (MQ=255) cTGTGTCAGTTCAAATACGGATTTACCGCCTTGAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCtt < 2:25513/143‑1 (MQ=255) tgtgTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTc > 1:375298/1‑143 (MQ=255) tCAGTTCAAAGACGGATTTACCGCCTTCAAAATTGTGGCGGGAGACCATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCa < 2:387299/143‑1 (MQ=255) ttCAAAGACGGATTTGCCGCCTTCAAAATTGTAGCGGGTGACGTTCCCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGa < 1:254006/143‑1 (MQ=255) tCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGAc > 1:558738/1‑143 (MQ=255) aCGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATg < 1:715831/143‑1 (MQ=255) aCGGAGTCAGCGCCTTCAAAAGTGTGGGGGGTGACGATACCAGCGTCGTCAACCTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATg < 2:258738/143‑1 (MQ=255) tACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATAGACAGTAGACAGACCAATTTCTTCACCCATATCGATCAGCCGTTTGTATAAATCATACGGACAGACGTGAAAGTTGTcg > 2:808427/1‑142 (MQ=255) tACCGCCTTCAAAAGTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGGAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTcc < 1:821690/143‑1 (MQ=255) tACCGCCTACAAAAGTGTAGCGGGTGACGATACCAGCGGCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTcc < 1:491627/143‑1 (MQ=255) ccGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGAACGCGATATACCGTAGACAGACCACGTTCTGCACCCATATCGATCAGACGTTTGTATCAATCTTCCGCACTTACTTGAGTGTTGTCCgc > 1:504625/1‑142 (MQ=255) cGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGc > 1:319350/1‑143 (MQ=255) cactgtgGCGGGGTACCACACCAGCGGCGTCAAACTCGTTCAGTACGCGATATACCGTACCCCGACCAATTTCTGCACCCATATCGATCAGACGTTTGTATAAATCTCCCGCACTGACGTGATGGTTGTCCGTCTCCTgaaga < 2:432004/140‑1 (MQ=255) aaTTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCCGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTgacga > 1:223877/1‑143 (MQ=255) aTTGTAGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTgaagaa < 1:476125/143‑1 (MQ=255) | TTTGCGGCAATTTCACGCTGACGCGCTTCGATGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAA > NC_000913/710297‑710573 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |