| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 759,250 | G→T | 100% | R182L (CGC→CTC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 759,250 | 0 | G | T | 100.0% | 66.2 / ‑6.7 | 28 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (15/12); total (16/12) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTC‑GGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA > NC_000913/759127‑759380 | tCGGTTCATGTGCCAGCGGCAAAGAAACCATGAAACGC‑GGCGAGCTGCGGGAGGCCCTCAAGCAAACCTACTGCGGCCCGATGGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATc < 2:300979/143‑1 (MQ=255) gTTCATTTGCCAGCGGCAAAGAAACCATGAAACGC‑CGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAg > 1:605128/1‑143 (MQ=255) ttCATGTGCCAGGGGCAAGGAAACCA‑GTAAGTCGGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACGGCGGCCCGATTGGTGCCGGGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGt < 2:760465/143‑1 (MQ=255) agttGCCACCGTCAACGAACCCATGAAACTC‑GGCGAGCTGCGGGAAGCCCTCCAGCACACCTACTGCGGCCTGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAATCTCTGGATCCAACAGCGTATCGAGTCTg < 1:78995/141‑1 (MQ=255) cAAAGAAACCATGAAACTC‑GGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCCCCGAAGGAAGACTCTGGATCCAACAGCGTACCCAGGCTCGTCGCCCGACtt > 2:511062/1‑143 (MQ=255) aaGAAACCATGAAACTC‑GGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTc > 1:593705/1‑143 (MQ=255) aaGAAACCATGAAACTC‑GGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGCACCGAGTAGATGCACATTACCAGCACCGCAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGCCGTTc > 2:351928/1‑143 (MQ=255) ccATGAAACTC‑GGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGc < 2:654510/143‑1 (MQ=255) aaaCTC‑GGCGAGCTGCTCGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTCGATCCCACAGCGTCGCCACGCTGGTCGCGCGACTTTCAATAGCGAaga < 1:839159/143‑1 (MQ=255) c‑GGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCGGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaaa < 2:777382/143‑1 (MQ=255) gacgCCCTCAAGCAAACGTACTGCCGCCCGATTGGTGCTGAGTATATGCACATTACCAGCACCGAAGAAAAGCTCGGAATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAg < 2:405382/140‑1 (MQ=255) aaGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGCACCCAACAGCGTATCGAGTCTGGACGCGCGACTTTCAATAGCGAAGAGAAAAAACACTTCTTAAGc > 2:527855/1‑143 (MQ=255) aGCCCTCAAGCACACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCg < 2:233365/143‑1 (MQ=255) cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGGGCACt > 1:438998/1‑143 (MQ=255) tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGCAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAGTAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTg > 1:811035/1‑143 (MQ=255) tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCACTAGCGAAGAGAAAAAACGCTTCGTACGCGCACTg > 1:660609/1‑143 (MQ=255) acgCAGACCTCCTGCGGCCCGATTGGTGCCGAGTATATGCACAGTACCAGCACCGAAGCCTAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAc < 2:764101/141‑1 (MQ=255) aaGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAc > 2:194645/1‑143 (MQ=255) aGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAcc < 1:616895/143‑1 (MQ=255) aGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAACGAACTGAca > 1:470989/1‑142 (MQ=255) ccTACTGCGGCCCGATTGGTGCCGAGCATATGCACATTACCAGCACTGAAGGAAAACTCTGGATCCAACAGGGTAGCGAGTCTGGTCGCGCGGGTTTCAAAAGCGAAGAGAAACAACGCTGCTTAAGCGAACGGAcggccgag > 1:220970/1‑141 (MQ=255) cccGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTg > 1:215643/1‑143 (MQ=255) ccGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGCCGCGCGACTGTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCCGAAGGCCATGAACGTTAccac > 2:341340/1‑141 (MQ=255) cgCACATTACCACCACCGAAGAAACACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCaaaa < 2:501922/142‑1 (MQ=255) cacaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAAtt > 1:432493/1‑143 (MQ=255) acacTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTc < 1:851360/143‑1 (MQ=255) tACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAACATTCCCTg > 2:454070/1‑143 (MQ=255) ccGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTACAATAGCGACGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGGTACCTCGGCGCATACTTCCCTGGAGCataa > 1:106106/1‑143 (MQ=255) | TCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTC‑GGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA > NC_000913/759127‑759380 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |