| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,637,646 | +T | coding (5/1500 nt) | pitA → | metal phosphate:H(+) symporter PitA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,637,645 | 1 | . | T | 96.9% | 89.7 / NA | 33 | L2L (CTA→TTA) | pitA | metal phosphate:H(+) symporter PitA |
| Reads supporting (aligned to +/- strand): ref base . (1/0); major base T (9/22); minor base G (1/0); total (11/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGC‑T‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCG > NC_000913/3637505‑3637782 | ataatataAGATAATTATTATTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGC‑tt < 2:451033/140‑2 (MQ=255) ataagataagTATTTTTTGAGTGAAATCCATCCAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGC‑ttacatg < 1:218291/143‑7 (MQ=255) ataAGATAATTAGTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACAtt < 2:635884/143‑1 (MQ=255) taAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTATATATTTCACTTAGCCCGCGCCGCGAAAGTCACTGATAATACGCCACGTTCATGTCCTATAAATCGCGTAACGGCCGATGC‑TGACAttt > 2:430489/1‑143 (MQ=255) aGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCACGCGCCGCGAAAGTGACTGATAATGCGCAGAGTTCATGTCCTCAAAATGGCGTAACGTCCTAGGCGT‑ACAtttgt > 2:245000/1‑143 (MQ=255) aGATAATTATTGTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACAtttgt < 1:250380/143‑1 (MQ=255) tatttattTTTTGAGTGTAATCCATACTGGGGGCAATTCAAACAAAGTCTATATTTCACTTTGCCCGCGCCGCGACAGTCACTGCTAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACAtttgtttg < 1:116207/140‑1 (MQ=255) ttttGAGTGAAATCCATGCAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTg < 1:392066/143‑1 (MQ=255) aGTGAAATCCATACAGGGCGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGAttt < 1:540352/143‑1 (MQ=255) ccATTCAGGGGGCAAATCAAAAAACGTCTATATTTCACTTTTCCGGCGCCGCGAAAGTCACTGATACTGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCg < 2:96747/143‑1 (MQ=255) aCAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCt < 1:23455/143‑1 (MQ=255) cAGGGGGCAAAGCAAGAAAAGTCTGTATGTCACTTTGCCCGCGCCCCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCCCAAAATGTCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTg < 2:470085/143‑1 (MQ=255) aTCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgc < 1:40212/143‑1 (MQ=255) aaaaaGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATCGCGTAACGTCCTATGCTT‑ACCTTTGTTTGCTGGCCTGGATGTGCCTACCGTGCTGATATTATTGCTTGCa > 2:321735/1‑143 (MQ=255) tatTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtg < 1:35953/143‑1 (MQ=255) tttCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtgtg < 2:493436/143‑1 (MQ=255) ttCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTCTGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGGTTTTGTGc > 2:191527/1‑143 (MQ=255) aCTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTattatt < 2:216573/120‑1 (MQ=255) aCTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTattatt > 1:216573/1‑120 (MQ=255) cgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGaa < 1:359859/143‑1 (MQ=255) cgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCAt < 2:230717/143‑1 (MQ=255) gcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTTCTTCCACTCGCTTTTGCGCCGTTCTACGAAGCCAta > 2:105131/1‑142 (MQ=255) aaGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATg < 1:394560/143‑1 (MQ=255) ggATAATGCGCCGCGTTCATGTCCTCAGAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGAGTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCa < 2:415712/142‑1 (MQ=255) tAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTAGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGa < 1:632244/143‑1 (MQ=255) aTGCGCCGCGTTCATGTCCTCCAACTGGCGTAATGTCCTTTGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGaca < 1:653534/143‑1 (MQ=255) cgccgcGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAg < 1:104739/143‑1 (MQ=255) gcgTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCaa > 1:527613/1‑143 (MQ=255) cATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCg < 1:611179/143‑1 (MQ=255) aTGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGt < 1:500344/143‑1 (MQ=255) tCCGCAAACTGGCGTAACGTCCTATCCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTGATTATTGCTTGCACTTGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGc < 2:551988/143‑1 (MQ=255) tCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAAc > 2:102299/1‑143 (MQ=255) gCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCAATCCATGGTTTTCATGACTCAGCCAACGCCGTGGCAACCGTTATCt > 1:121008/1‑143 (MQ=255) tAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGGGGCAACGGTTATCtata > 2:64587/1‑143 (MQ=255) tATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGGTCTACGAAGCCATCAATGGTTTCCGTGCCACAGCCAACGCCGTGGCCCCCGTTCTCTCTACCcgcgcg > 1:641139/1‑143 (MQ=255) | ATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGC‑T‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCG > NC_000913/3637505‑3637782 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |