Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,661,969 C→G V55L (GTT→CTT)  iscR ← DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,661,9690CG92.9% 72.3 / NA 29V55L (GTT→CTT) iscRDNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base G (17/9);  total (18/11)
Fisher's exact test for biased strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCA  >  NC_000913/2661841‑2662105
                                                                                                                                |                                                                                                                                        
gccgctTTTTCCTTGATAACGGGTGGCATCTACGGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTAc                                                                                                                            <  1:603201/143‑1 (MQ=255)
   gccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGAACGCTGGAAAGCAGACCATTTTTACGCa                                                                                                                         >  1:112898/1‑143 (MQ=255)
                  aCGGGTGGCATCTTGAGTTGCGGCGACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAgtt                                                                                                          <  2:2336/143‑1 (MQ=255)
                  aCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAgtt                                                                                                          >  2:607966/1‑143 (MQ=255)
                             cTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATaa                                                                                               <  1:607966/143‑1 (MQ=255)
                              tACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGCTGCTGCTGGCATCTTTGCCTATCAGATAACCAAAGCCTAGACCACATACGCTGGAAAACAAACAAATTTTACGCAGACCGGAAAACAGTTGTTCCCGATAAg                                                                                              >  1:293952/1‑143 (MQ=255)
                                 aGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTGCAGATAAGaca                                                                                           >  1:357449/1‑141 (MQ=255)
                                        tCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTTTTTTTTTGTTCAAACAGTTGTTCCAGATCAGTAAGGGAAAt                                                                                    >  2:264106/1‑143 (MQ=255)
                                                 cTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACg                                                                           >  1:528453/1‑143 (MQ=255)
                                                       cctgcGCCAACGGCGATGCTGCTCGCATCTTTGCCGAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTTGGa                                                                     <  2:476788/139‑1 (MQ=255)
                                                       aCTTCGCCAACGGCGATGCTGCTGGTTTGTTTGCCCAACACATAACCACCGCCTGGTCCACGTACGCTGGACAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGCTAAGAAAGGGAAATTCCCTGACGCTCGGa                                                                     >  2:143071/1‑143 (MQ=255)
                                                           cGCCCACGGCGCTGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGCTGTTCCAGACAAGAAAGGGAAATTCCCTGACGTTCGGAAata                                                                 >  2:237744/1‑143 (MQ=255)
                                                               aaCGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAACGCAGACCATTTTTACGCAGACGGAAAAAAAGTTGTTCCAGATAAGCAAGGGAACTTCCCTGACGACCGCAAATAGCAg                                                             >  1:117828/1‑143 (MQ=255)
                                                                          ttctagCATCTTTGTCTAACCGATAAGCACCGGCTTGTCCACGTTCGCGCGAAACCAGACCATTTTTACGCAGACGGGAAAACATTTGTTCCAGATTAGAAAGGGAAATTCCCTGACGTTCGGAAATATCATCCAACGGTAcc                                                  <  2:100550/138‑1 (MQ=255)
                                                                             tgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCggg                                               >  1:551325/1‑143 (MQ=255)
                                                                                    cgattcTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCtt                                        <  1:535952/138‑1 (MQ=255)
                                                                                            aCAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTg                                >  1:101555/1‑143 (MQ=255)
                                                                                              aGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTAACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAg                              >  2:93584/1‑143 (MQ=255)
                                                                                               gagaaCCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTGCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgc                             <  1:438884/140‑1 (MQ=255)
                                                                                                aTAACCACCGCCCGGTCCACGTACGCTGGAAAGCAGACCCTTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgcg                            <  1:121320/143‑1 (MQ=255)
                                                                                                  aaCCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCa                          >  2:51932/1‑143 (MQ=255)
                                                                                                     caccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACg                       >  1:435588/1‑143 (MQ=255)
                                                                                                      accGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGCAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGAGCAACGt                      >  1:71120/1‑143 (MQ=255)
                                                                                                              gTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCAtt              >  1:615770/1‑143 (MQ=255)
                                                                                                              gTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAAAAGTTGTTCCAGGTAAGAAAGGGAAATTCCCTGACGTACGGAAATAGCAGCCGACGGTAGCGGGACCGCTTCAGAGGTGAGCGCAGCGTCAAACaat              >  1:57577/1‑141 (MQ=255)
                                                                                                              gTCCACACACGCTGTATAGCAGACTTTTTTTACGCGGACTGGAAACCAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGTAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCAtt              <  2:481388/143‑1 (MQ=255)
                                                                                                                      aCGCTGCAAACCAGACCCTTTTTACTCAGACGGTATAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGATGTTCGGAAATATCAGCTAACGGTACCGGGCCCGCTTCAGAGTTTAGCGCAACGTCAAGCATTGCGGTCAc      <  2:393319/143‑1 (MQ=255)
                                                                                                                        gCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACgg    <  1:51932/143‑1 (MQ=255)
                                                                                                                          tGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCa  >  2:342885/1‑143 (MQ=255)
                                                                                                                                |                                                                                                                                        
GCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCA  >  NC_000913/2661841‑2662105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑