Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,632	T→A	V107E (GTG→GAG)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,632	0	T	A	96.4%	70.4 / NA	28	V107E (GTG→GAG)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (11/16);  minor base G (0/1);  total (11/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCT  >  NC_000913/3041502‑3041767                                                                                                                                   |                                                                                                                                        gACGCCAAAGGTAAAATGTGGAGCAATTCACGTCTGTTTCGCGCGGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAGCTGAGAAAATATGCGGTATTCTCTAGAGAGACGATGGCGcc                                                                                                                             >  1:28517/1‑143 (MQ=255)              aaaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCgt                                                                                                                >  1:362408/1‑143 (MQ=255)              aaaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCgt                                                                                                                >  1:363346/1‑143 (MQ=255)                aTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGCACCGCAGCTGACTGAACTGAAAAAGTATGCGGTGTTCTCTGAAGAGACCATCGCGCCAGACGACGAGGgtgt                                                                                                              >  1:179207/1‑143 (MQ=255)                      aGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAGAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTggtgct                                                                                                        >  1:594557/1‑143 (MQ=255)                                gTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATTCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGcc                                                                                              <  1:627576/143‑1 (MQ=255)                                    gTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGtt                                                                                          <  1:140551/143‑1 (MQ=255)                                     ttCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTACGTGAACTTCTTCTGACAGAACTTTAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGttt                                                                                         <  2:467727/143‑1 (MQ=255)                                         gcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGCGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAg                                                                                     >  1:278334/1‑143 (MQ=255)                                              ggCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGAACATCGCGCCAGACGACGAGCGTCTGCTGCTTGGTGATGCCGCATTCCAcgcgcg                                                                                >  2:125434/1‑143 (MQ=255)                                                cGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCGAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcg                                                                              >  2:106159/1‑143 (MQ=255)                                                               taTTGAACGGCGCAGCGCGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAAACAATCAAGTTAGTCTTTGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtc                                                               <  1:592527/142‑1 (MQ=255)                                                                 ttGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtctc                                                             >  1:216412/1‑143 (MQ=255)                                                                  tGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct                                                            <  1:282580/143‑1 (MQ=255)                                                                    tgcGGCGCAGCGTGCGTGAACCGCAGCTGACTGGACTGAGATAGGTGGCTGTATGCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGTTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCttt                                                          <  2:260764/141‑1 (MQ=255)                                                                          gcAGCGTGCGTGAACCGCAGCTGCCTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGaa                                                    <  2:98929/143‑1 (MQ=255)                                                                           cAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTACCGGTTTTCAGGCGCGCGCCGCGCTGGAAAGTCTCTTTGGCGGAc                                                   >  1:408368/1‑143 (MQ=255)                                                                                tgcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCt                                              <  1:449443/143‑1 (MQ=255)                                                                                    tgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCga                                          <  1:402307/143‑1 (MQ=255)                                                                                     gAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaa                                         <  1:54564/143‑1 (MQ=255)                                                                                            aGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGaaaaa                                  <  1:206282/143‑1 (MQ=255)                                                                                                gactgaACTGAAAAAATATGCGGTATTCTCTCAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGACGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAgg                              <  2:429663/143‑1 (MQ=255)                                                                                                gactgaACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGCGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAgg                              <  1:333187/143‑1 (MQ=255)                                                                                                gactgaACTGAAAAAATATGCGGTATTCCCTAACAAGACCAGCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAgg                              <  1:334153/143‑1 (MQ=255)                                                                                                        tgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTACGAGGCGGCGGGTGTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGGAAGACAAACCGGTAGTCaaa                      >  1:415215/1‑143 (MQ=255)                                                                                                             gagtgtGCGGTCTTGTCTGAGGGGACGATCGCGGCAGACGACGAGCGTGGGCTGCTTGGTGTTGCCGGTCTTCAGGCACGCGCCGAGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAgg                 <  2:612109/138‑1 (MQ=255)                                                                                                                tatGCGGTATTCTCTCAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgcgc              <  1:139237/143‑1 (MQ=255)                                                                                                                            tctAACGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGCTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACCGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCt  <  2:420789/143‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        GACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCT  >  NC_000913/3041502‑3041767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑