Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,158,785 C→T P99L (CCC→CTC)  oxyR → DNA‑binding transcriptional dual regulator OxyR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,158,7850CT96.8% 81.4 / NA 31P99L (CCC→CTC) oxyRDNA‑binding transcriptional dual regulator OxyR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (16/14);  minor base G (0/1);  total (16/15)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATC  >  NC_000913/4158647‑4158924
                                                                                                                                          |                                                                                                                                           
gTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGAGCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACTACGTCCGGACCGCTGCACATTGGTTTGATTCTcaca                                                                                                                                         <  1:341489/143‑1 (MQ=255)
    aGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGCGATGGCAAGCCCGCAGGGCGAGAGGATGTCCGGACCGCTGCACATTGCTTTGATTCTCGCACTTg                                                                                                                                     >  2:498509/1‑143 (MQ=255)
        ttgttCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGAcc                                                                                                                                 >  1:573972/1‑143 (MQ=255)
          gttCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGGGAAAGTCCTTAAAGAGATGGCAAGCCAGCGGGGCGAGACGATGGCCGGACCGCTGCACACTGGTTTGATTCTCACAGTTGGACCGt                                                                                                                               >  2:13915/1‑143 (MQ=255)
          gttCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGGGCTGCGTGAGGTGAAAGTCCTGAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGCTTGTCACAGGTGGAGCGt                                                                                                                               >  2:400885/1‑143 (MQ=255)
            tCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCGTAc                                                                                                                             >  1:381568/1‑143 (MQ=255)
                 cAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGGCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTCTGATGCTCACAGTTGGACCGTACCTGcg                                                                                                                        >  2:521199/1‑142 (MQ=255)
                  aGGCGGGAATGCGGCTGGGGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTGAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCGTACCTGCTa                                                                                                                       <  1:231479/143‑1 (MQ=255)
                       ggAATGCTGCTCGTGGCTCATCCTCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCGTACCTGCTACCGCa                                                                                                                  <  1:620267/143‑1 (MQ=255)
                              tgctgGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCGTACCTGCTACCGCATATTATc                                                                                                           <  1:635819/143‑1 (MQ=255)
                                   gtggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTAt                                                                                                      >  1:68747/1‑143 (MQ=255)
                                            gtgcgTACCGTGCTCCGAGAGCTGAAAGTCCTTTATGATATGGCAAGCTAGCAGCTCGAGACGATGTCCGGATCGCTGCACATTGTTTTGATTCTCACAGTTGGACCGTACTTGCTACCGCATATTATCCCTATGCTGCACCa                                                                                             <  2:13683/141‑1 (MQ=255)
                                            gcgcGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCa                                                                                             >  1:131905/1‑143 (MQ=255)
                                                 tACCGTGCTGCGTGCGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGGCGATGTCCGGACCGCTCCACATTGGTTTGATTCTCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCt                                                                                        <  1:212323/143‑1 (MQ=255)
                                                   ccGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCatt                                                                                      <  2:204921/143‑1 (MQ=255)
                                                        ctgcATGAGGTGACAGTCCTTAAAGAGATCGCTAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCaaa                                                                                 <  2:262010/143‑1 (MQ=255)
                                                          gcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCCTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCACAGc                                                                               >  2:63355/1‑143 (MQ=255)
                                                            gggAGGGGGAAGGCATTAAAGAGAGGGCGAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTCGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTg                                                                             <  1:81671/141‑1 (MQ=255)
                                                                 gTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGAGGTCCGGACCGCTGCACATAGGTTTGATTCTCACCGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTGTCCAAAGCGGGaaag                                                                        >  2:597342/1‑142 (MQ=255)
                                                                         ccTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCGCCAGACCTTTCCAAAGCGGGAACTGTGTCTGc                                                                >  2:500817/1‑143 (MQ=255)
                                                                           ttAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCAt                                                              >  2:443748/1‑143 (MQ=255)
                                                                            tAAAGAGATGGCAAGCCAGCAGGGCGTGACGATGTGCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGACACGTATCTGCATg                                                             <  2:616003/143‑1 (MQ=255)
                                                                             aaaGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTACACAGTGGTTTGATTCTCACAGCTGGGCCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTACAACGCTGGAAGAGTATCTGCATGa                                                            >  1:518680/1‑143 (MQ=255)
                                                                              aaGAGACGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGaa                                                           <  1:213809/143‑1 (MQ=255)
                                                                               agagATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAg                                                          >  1:616640/1‑143 (MQ=255)
                                                                                  gaTGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGAAAATGTATCTGCATGACGctc                                                       >  1:330251/1‑141 (MQ=255)
                                                                                          gCCAGCAGGGCGAGGCGATGTCCGGACCGCTGCACATTGGTTTGATGCGCACAGTTGGACCGTACCTGCGACCGCCTATTATCCCCATGCTGCACCAGAGCTTTCCAAAGCTGGAAATGGATCTGCATGAAGCACAGACccac                                               <  2:9962/143‑1 (MQ=255)
                                                                                                          gATGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAACTGTATCTGCATGAAGCCCAGCCCCCCCCGTTCCTGGCGCCAc                               >  1:252036/1‑143 (MQ=255)
                                                                                                            tGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTg                             <  2:147491/143‑1 (MQ=255)
                                                                                                            tGTCCGGACCGCTGCACATTGGTTTGATTCTCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTg                             <  1:330109/143‑1 (MQ=255)
                                                                                                                                       ttCTCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATc  <  1:143970/143‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
GTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATC  >  NC_000913/4158647‑4158924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑