Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,341,824	T→G	intergenic (‑196/‑87)	melR ← / → melA	DNA‑binding transcriptional dual regulator MelR/alpha‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,341,824	0	T	G	100.0%	130.5 / NA	41	intergenic (‑196/‑87)	melR/melA	DNA‑binding transcriptional dual regulator MelR/alpha‑galactosidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/22);  total (19/22)

CGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTT  >  NC_000913/4341686‑4341960                                                                                                                                           |                                                                                                                                         cGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGACAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGGTCTGAGTGTATg                                                                                                                                      >  1:638157/1‑143 (MQ=255)  gggTGGGAGGACGGTTTGCACCCTCTTCCCCGAGGGGCGATGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATgg                                                                                                                                     <  2:638157/141‑1 (MQ=255)  gAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATgg                                                                                                                                     <  2:154642/143‑1 (MQ=255)       ggAGCACGCTTTCCACCGTCTTCCCAGAGGGGCGAGGGGACTCTCGGAGTATCATGAGGCCGAAAACCCTGTATTTCATGTAATGTATGCCCATAAGCTAAGGTTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATg                                                                                                                                <  2:434045/143‑1 (MQ=255)        gAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGAGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGc                                                                                                                               <  1:500118/143‑1 (MQ=255)            aCGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGTCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCCGAGTGTATGGGAATGCTCaa                                                                                                                           >  1:12826/1‑143 (MQ=255)                ttttCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTg                                                                                                                       >  2:297752/1‑143 (MQ=255)                  ttCGCCCTCTGCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATGTATTCGCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGa                                                                                                                     <  1:46011/143‑1 (MQ=255)                   tCACCCGCTGCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGaa                                                                                                                    <  2:634303/143‑1 (MQ=255)                        ntctTNCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCNCATAAACTCAGATTTANTGCTGCTTCNCGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCgg                                                                                                               <  1:562233/142‑1 (MQ=255)                        cttttgccAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAATACTCTGCTTTTCAGGTAATTGATGCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCgg                                                                                                               <  2:145628/137‑1 (MQ=255)                          catgccAGAGGGGCGAGGGGAAGCACGGAGTATCATGAGGCCGAAAACTCTGCGATTCAGGGAATTGATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAg                                                                                                             <  1:4162/139‑1 (MQ=255)                              ccAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGttt                                                                                                         >  1:182437/1‑143 (MQ=255)                               ccgagGGGCTAGGGTACTTTCCGATTATCATGTGGCCGAAAACTCTGCTTTTCAGGTAATTTACTCCCATAAACTCAGAGTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGtttt                                                                                                        <  2:216505/141‑1 (MQ=255)                                  agGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTg                                                                                                     <  1:460035/143‑1 (MQ=255)                                    gggCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCa                                                                                                   <  2:363064/143‑1 (MQ=255)                                    gggCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCa                                                                                                   <  2:51262/143‑1 (MQ=255)                                             gACTCTCCGAGTATCATGAGGCCGAAAACTATGCTTCTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGCATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGCGGTTTTCTGCAGATTCGTCt                                                                                          >  2:613774/1‑143 (MQ=255)                                               ctctCCGTGTATCATGAGGCCGTAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGc                                                                                        <  2:435883/143‑1 (MQ=255)                                                  tcCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCCACCGGGAAGCAGGAGGTTTTCTGCAGATTCGCCTGCCag                                                                                     >  1:176450/1‑142 (MQ=255)                                                               aGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTc                                                                        <  2:113969/143‑1 (MQ=255)                                                                   cGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTcaagc                                                                    <  2:171615/143‑1 (MQ=255)                                                                     aaaaCTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTcaagcaa                                                                  <  1:344663/143‑1 (MQ=255)                                                                           ctGCTTTTCAGGTAATTTATTCCCATACACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAgg                                                            <  1:233198/143‑1 (MQ=255)                                                                              cTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGCGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGgaga                                                         >  2:608518/1‑143 (MQ=255)                                                                               tgttCAGGTCATTAATTCCCAGAAACTAAGTTGTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGAt                                                        <  2:227311/141‑1 (MQ=255)                                                                                 ttCAGGGAATTTAGTCCCATACGCTCAGAGTTACTGCTGCGTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCt                                                      <  1:17074/143‑1 (MQ=255)                                                                                       tAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAGGCAAGCCAGGAGATCTGCatga                                                >  1:265312/1‑143 (MQ=255)                                                                                       tAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCatga                                                >  1:200877/1‑143 (MQ=255)                                                                                          tttATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTACCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCAAGATGt                                             >  1:576681/1‑143 (MQ=255)                                                                                              ttCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGAAGAAGTTATTCAAGCAAGCCAGCAGCTCTGCATGATGTCTGc                                         >  2:428959/1‑143 (MQ=255)                                                                                                   aTAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCa                                    >  2:41854/1‑143 (MQ=255)                                                                                                         tCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTa                              >  1:114982/1‑143 (MQ=255)                                                                                                           aGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACa                            >  1:591565/1‑143 (MQ=255)                                                                                                              tttACTGCTGCTTCACGCAGGACCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGCAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAACATTACAttt                         >  1:65816/1‑143 (MQ=255)                                                                                                                     ctgctTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGgcg                  >  2:147060/1‑143 (MQ=255)                                                                                                                     ctgctTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGgcg                  <  1:297752/143‑1 (MQ=255)                                                                                                                        ctTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTg               >  1:67279/1‑143 (MQ=255)                                                                                                                              gCAGGCTCGGGGTGTAGGGGAATGCTCAACCTGGAAGCCGGGGGTGTTCTGCAGACTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTTTGCACCCAAAATTACATTTATCGGCGCTGGTTcga         <  1:135904/143‑1 (MQ=255)                                                                                                                              gCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGCTCTGCATGCTGTCTGCGCCCCGTATTACATTTATCTGCTCTGGTTaga         >  1:50091/1‑143 (MQ=255)                                                                                                                                     cTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGCGATCTGCATGATGTCTGCACCCAAAACTACATTTATCGGCGCTGGTCCGACGAtttc  >  1:291452/1‑142 (MQ=255)                                                                                                                                           |                                                                                                                                         CGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTT  >  NC_000913/4341686‑4341960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑