| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 759,250 | G→T | R182L (CGC→CTC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 759,250 | 0 | G | T | 96.5% | 72.5 / ‑4.8 | 29 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (14/14); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01 | |||||||||||
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCC > NC_000913/759110‑759369 | ccAGGGGACCTTCATGGGCGGGTAGTTTCGCAGCGGCAAAGAAACCATGAAACTTGGCGTGCTGCTGGAAGCCCTCAAGCAAACCTACTTCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct < 2:39977/143‑1 (MQ=255) ggAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGTAAAACTCTGGa < 1:474237/143‑1 (MQ=255) gggaCCTCCAACGTCGGTTCATGTGCCTGCGGTAACGAAACCATGAATCTCGGCGTGCTGCTGGAAGCCCTCAAGCACACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGAt < 2:439062/141‑1 (MQ=255) ccTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCaa < 1:595054/143‑1 (MQ=255) cggcGGTTCATGTGCCAGCGGCAAAGAAACCATGAAACGCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTa < 2:238133/140‑1 (MQ=255) tCGTTTCCTTTGCCAGCGGCAAAGAAACGATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATc < 1:383340/143‑1 (MQ=255) ttCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCCGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAgg > 1:228539/1‑142 (MQ=255) aGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGACCCGATTGGTGCCGAGCATATGCGCACTACCAGCACCGAAGAAAAACTCTGGATCCGACAGCGTATCAGGGCTGGTcgcgc > 1:20854/1‑143 (MQ=255) gCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACt > 1:575902/1‑143 (MQ=255) aaaGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTCTATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGGCttt > 1:196526/1‑143 (MQ=255) aaaCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTGGTCGCGCGCCTGTCCAt > 2:86347/1‑143 (MQ=255) aatcATGAAACTCGGCTAGCTGCTGGAAGCCTTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATa < 2:113526/140‑1 (MQ=255) aCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGCGTATATGCACATTACCAGCCCCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTGTCCATAg > 1:148539/1‑143 (MQ=255) aaaCTCGGCGCGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAaga < 1:41224/143‑1 (MQ=255) ctgctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGctt < 1:138831/143‑1 (MQ=255) tgctgGAAGCCCACCAGCAATCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGcttc < 1:586132/143‑1 (MQ=255) ccTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAACCTCTGGATCCAACAGCGTCTCGAGTCCGGCCGCGCGACTTCCACTAGCGAAGAGAACAAACGCTTCTTAAGCGCAc > 2:87729/1‑143 (MQ=255) cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAACAACGCTCCTTAAGCGAACt > 2:601427/1‑143 (MQ=255) caGCGAACCTACTGCGGCCCGAGTGGTGCAGAGTATATGCACAGTACCAGCGCCGAAGAAGACCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAc < 2:480068/142‑1 (MQ=255) aaGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAc > 1:521229/1‑143 (MQ=255) aaCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGCGTCTGGTCGCGCGACTTTCAATAGAGGAGAGAAAAAACGCTTATTAAGCGAACGGAccgcgg > 1:164408/1‑141 (MQ=255) aaCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGGCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGGccgccg > 1:164321/1‑143 (MQ=255) cTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTCCCAGCACCGACGAAACACTCTGGATCCACCAGCGTATCGCGTCTGGTCGCGCGACTTTCAATAGCGAAGCGAAAAAACGCTCCTTAAGCGAACTCACccccgccg > 1:205773/1‑141 (MQ=255) cTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTCAAGCGAACCGACggccgcgg > 2:484871/1‑141 (MQ=255) gCCCGATTGGTGCCCACTATATGCACATCACCAGCACCGAAGAAACACTCTGGATCCAACAGCGTATCTAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCtt < 1:11207/143‑1 (MQ=255) ccGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATACAACAGCATATCGAGTATGGTAGCGCGACTTTCCCTAGCGAAGAGAAAAAACGATTCTTAAGCGAACTGACCTCCGAAGAGGGTCTTGAACGTTAcggc > 2:619948/1‑140 (MQ=255) ttttttttGCACATTACCAGCACGAAAGAAAAACTCTGGATCCAACAGGGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCgg < 1:231149/136‑1 (MQ=255) gTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgcg > 2:309507/1‑143 (MQ=255) caTTCCCAGCACCGAAGAAAAACTCCGGATCCAACAGCGTATCAAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTcc < 2:508005/143‑1 (MQ=255) caTTACCAGCACCGAAGAAAAACTCTGGATCCAACCGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTcc < 1:617725/143‑1 (MQ=255) | CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCC > NC_000913/759110‑759369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |