Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,637,645:1 +T 100% coding (4/1500 nt) pitA → metal phosphate:H(+) symporter PitA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,637,6451.T100.0% 153.8 / ‑6.7 51L2L (CTA→TTA) pitAmetal phosphate:H(+) symporter PitA
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base T (21/29);  minor base G (1/0);  total (22/29)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGC‑TACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCG  >  NC_000913/3637511‑3637780
                                                                                                                                       |                                                                                                                                       
taAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACAttt                                                                                                                                  <  2:417297/143‑1 (MQ=255)
taAGATAATGATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACAttt                                                                                                                                  <  2:417084/143‑1 (MQ=255)
  aGATAATGATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACAtttgt                                                                                                                                <  2:706481/143‑1 (MQ=255)
   gATAATTATTTTTTGAAAGAAAACCAACCAGGGGGCCGCCCAAAAAACGTCTATATTTCACTTTGCCCGCGCGGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACAtttgtt                                                                                                                               <  2:102213/143‑1 (MQ=255)
     tAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACAtttgtttg                                                                                                                             <  1:177680/143‑1 (MQ=255)
       attattTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCt                                                                                                                           <  1:307194/143‑1 (MQ=255)
        ttattTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTg                                                                                                                          >  1:412398/1‑143 (MQ=255)
          attTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTCTGCTTACATTTGTTTGCTGGc                                                                                                                        >  2:483222/1‑143 (MQ=255)
               ttGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGa                                                                                                                   <  2:74194/143‑1 (MQ=255)
               ttGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGa                                                                                                                   >  1:775888/1‑143 (MQ=255)
                tGAGCGAATTCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCCAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGAt                                                                                                                  <  1:459534/143‑1 (MQ=255)
                  aGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGGttt                                                                                                                >  2:474158/1‑143 (MQ=255)
                        aTCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATAc                                                                                                          >  1:644255/1‑143 (MQ=255)
                         tCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCAAGCGCCGCGAAAATCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCGTATGCTTACATTTGGTTGCTGGCCTGGGTTTGGATagc                                                                                                         >  1:773591/1‑141 (MQ=255)
                               cAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATGTGTTTGCTGGCCTGGGTTTGCATACCGGGCTg                                                                                                   >  1:590240/1‑143 (MQ=255)
                               cAGGGGGCAAACCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTg                                                                                                   <  1:650938/143‑1 (MQ=255)
                                 ggggggAAGTCAAAAAAAGTCTATATTTCCCTTTCCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGtt                                                                                                 <  2:530875/143‑1 (MQ=255)
                                   gggCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGttat                                                                                               <  1:178935/143‑1 (MQ=255)
                                     tcAAATAAAGAAAGGTCTATATTTCGCTTCGCTCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCATCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGttatta                                                                                             <  2:901066/142‑1 (MQ=255)
                                        aaTCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCCTACCGGCCTGTGcttattt                                                                                          >  2:726639/1‑142 (MQ=255)
                                        aaTCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttg                                                                                          >  1:771411/1‑143 (MQ=255)
                                        aaTCAAAAAAAGTCTATATTTCACTTTCCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttg                                                                                          >  1:300521/1‑143 (MQ=255)
                                         aTCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCGATGCTTGCATTTGGTTGCGGGCCTGGATTTGCATACCGGGCTGGTATTAttgc                                                                                         >  1:438577/1‑143 (MQ=255)
                                          tCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgct                                                                                        >  2:36711/1‑143 (MQ=255)
                                           cAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgctt                                                                                       <  1:36711/143‑1 (MQ=255)
                                            aaaaaaaGTCTATATGTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgcttg                                                                                      <  2:874159/143‑1 (MQ=255)
                                                    tCTATATTTCGCTTTGCCCGCGCCGTGCAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCt                                                                              >  2:505605/1‑143 (MQ=255)
                                                    tCTATATTTCACTTTGCCCGCGCCGCGAAAGGCACTGATAATGCGCCGCGTTCATGTCCTCGAAATGGCGTAACGTCCGGTGCTTAGATTTGTCTGCTGGGCTGGATTGGCGTACCGCGCTGTTGTTATTGCTTGCGCGGGcc                                                                              >  1:387144/1‑142 (MQ=255)
                                                      tataTTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTACCTCGACGGTGGTTGCCTACAGGCTTGCAGTTATTGCTTGCACTGGCttt                                                                            >  1:339908/1‑143 (MQ=255)
                                                       agatgtCACCTTGCCCGCGCCCCCAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCtttt                                                                           <  2:10524/138‑1 (MQ=255)
                                                          tttCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtgtg                                                                        >  2:563702/1‑143 (MQ=255)
                                                               cTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGtt                                                                   <  1:561133/143‑1 (MQ=255)
                                                                      cgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGaa                                                            <  1:350339/143‑1 (MQ=255)
                                                                      cgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGaa                                                            <  2:469910/143‑1 (MQ=255)
                                                                            gcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATc                                                      <  1:42786/143‑1 (MQ=255)
                                                                             cgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAAAGGCGTAACGTCCTATGCGTACATTTGTTTGCTGGCGTGGATCTGCATACCGGGCCGTTATTATTGCCTGCCCTGGTCTTTTTGCTGGTCTACGCAGCCCTcc                                                     >  2:595528/1‑142 (MQ=255)
                                                                              gAAAGTCACTGATAACGCGCCGCGTTCACGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTCGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGACATCaa                                                    <  1:561806/143‑1 (MQ=255)
                                                                               aaaGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAAt                                                   <  2:259354/143‑1 (MQ=255)
                                                                                     aCTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTc                                             >  1:705542/1‑143 (MQ=255)
                                                                                      cTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTcc                                            <  2:644255/143‑1 (MQ=255)
                                                                                              gcgcCACGGTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACAATAGCTTACTGGCCTGGATTTGCATACCGGCCTGTCATTATTGCTTGCACTGGCTGTTGTGCTGTACTACGAAGCCATCAATGGTTACAATGAcaca                                    >  2:701072/1‑143 (MQ=255)
                                                                                                gccgcGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACTCAGc                                  <  1:178223/143‑1 (MQ=255)
                                                                                                  cgcgTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGCTATTATTGCTTGCACTGGCCTTTGTGCTGTTCTACGAAGCCACCAATGGTTTCCATGCCCCAGCCa                                >  2:111847/1‑143 (MQ=255)
                                                                                                              cTCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCaa                    >  1:87007/1‑143 (MQ=255)
                                                                                                               tCAAAATGGCGTAACGTCCTATGCTTACATTTGTTTGCTGGCATGGATTTGCATACAGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCCacgacgcgactac                   >  1:76366/1‑136 (MQ=255)
                                                                                                                       gCGTAACGTCCTAGGCTTACCTTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCt           <  2:422844/143‑1 (MQ=255)
                                                                                                                       gCGTAACGTCCTAGGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCGGTTATTATTGCTTGCGCTGGCTTTTGTGCTGTTCTACGGAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCt           <  2:423210/143‑1 (MQ=255)
                                                                                                                          tAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCtata        <  2:771411/143‑1 (MQ=255)
                                                                                                                          tAACGTCCTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCtata        <  2:300521/143‑1 (MQ=255)
                                                                                                                               tCCTATGCTTACTTTTCTGTGCTGGCCTGTATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCcgc   <  2:265814/143‑1 (MQ=255)
                                                                                                                                ccTATGCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCACACGCCGTTGCAACCGTTATCTATACCcgcg  <  2:803714/143‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGC‑TACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCG  >  NC_000913/3637511‑3637780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑