BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,309,640	A→G	12.7%	I210I (ATT→ATC)	kch ←	K(+) channel Kch

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,309,640	0	A	G	12.7%	69.4 / 3.3	33	I210I (ATT→ATC)	kch	K(+) channel Kch
Reads supporting (aligned to +/- strand): ref base A (12/15); new base G (2/2); total (15/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

TATTGATGGCGAGAATCGAATGTCCGCAAACAATAAAATGATCTTTACGATGCATTGTATGATTGTTTCCTTTTACAAGTTTGTTGAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCATAGCCGACGGTTGACATGGTTTCTATCGAAAAATAGAACGCGGTCATCAAACTTTCTATTCGCGGATTAAA  >  NC_000913/1309498‑1309777

tATTGATGGCGAGAATCGAATGTCCGCAAACAATAAAATGATCTTTACGATGCATTGTATGATTGTTTCCTTTGACAAGTTTGTTGAATCCCCCGCGGCTAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAGACGGTa                                                                                                                                           >  1:641784/1‑143 (MQ=255)

  ttGATGGCGAGAATCGAATGTCCGCAAACAATAAAATGATCTTTACGATGCATTGTATGATTGTTTCCTTTTACAAGTTTGTTGAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAAt                                                                                                                                         <  1:862571/143‑1 (MQ=255)

          gagaATCGAATGTCCGCAAACAATAAAATGATCTTTACGATGCATTGTATGATTGTTTCCTTTTACAAGTTGGTTGAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGaaa                                                                                                                                 <  2:427141/143‑1 (MQ=255)

           agaATCGAATGTCCCCAAACAATAAAATGATCTTTACGAGGCACTGAATGATTGTTTCGTTTTACAAGTTTGTTGAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAaat                                                                                                                                <  1:767353/143‑1 (MQ=255)

            gaATCGAATGTCCGCAAACAATAAAATGATCTTTACGATGCATTGTATGATTGTTTCCTTTTACAAGTTTGTTGAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTGATGCCGGAaata                                                                                                                               >  2:107382/1‑143 (MQ=255)

            gaATCGAATGTCCGCAAACAATAAAATGATCTTTACGATGCATTGTATGATTGTTTCCTTTTACAAGTTTGTTGAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTGATGCCGGAaata                                                                                                                               >  2:904167/1‑143 (MQ=255)

             aaTCGAATGTCCGCAAACAATAAAATGATCTTTACGATGCATTGTATGATTGTTTCCTTTTACAAGTTTGTTGAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCAGGAaataa                                                                                                                              >  2:823693/1‑143 (MQ=255)

                  aaTGTCCGCAAACAATAAAATGATCTTTACGATGCATTGTATGATTGTTTCCTTTTACAAGTTTGTTGAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGAcc                                                                                                                         >  1:247930/1‑143 (MQ=255)

                            aaCAATAAAATGATCTTTACGATGCATTGTATGATTGTTTCCTTTTACAAGTTTGTTGAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGa                                                                                                               <  1:442657/143‑1 (MQ=255)

                                 tAAAATGATCTTTACGATGCATTGTATGATTGTTTCCTTTTACAAGTTTGTTGAATCCCCCGAGGATAAGCGGATCAAAAATTGGGGTCATAGCTGTGGCAAAGACGGTCATGCAACAATTATTGACGGAACGAGAGAACaag                                                                                                          >  2:145169/1‑142 (MQ=255)

                                         tCTTTACGATGCATTGTATGATTGTTTCCTTTTACAAGTTTGTTGAATCCTCCGGGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAAGGATCGTGCTGa                                                                                                  >  1:916960/1‑143 (MQ=255)

                                                    cATTGTATGATTGTTTCCTTTTACAAGTTTGTTGAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAg                                                                                       >  1:319565/1‑143 (MQ=255)

                                                     aTTGTATGATTGTTTCCTTTTACAAGTTTGTTGAATCCCCCTCTGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAgg                                                                                      <  1:539626/143‑1 (MQ=255)

                                                                  ttCCTTTTACAAGTTTGTTGAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCa                                                                         >  2:836223/1‑143 (MQ=255)

                                                                   gtcTTTAACAAGTTTGATGAATACCCCGGGGATAAGCGGGCCAAAAATTGAGTTCATAGATGTGGCGAAAACGGTCATGCCTGAAATAATGACCGAAATAGGGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCAt                                                                        <  2:152795/141‑1 (MQ=255)

                                                                    ccTTTTACAAGTTTGTTGAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATAACGCCATa                                                                       >  2:138701/1‑143 (MQ=255)

                                                                      tgttACAAGTTTGTTGATTCCCCCGCGGATAAGCGTACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCATAGc                                                                     <  2:629464/141‑1 (MQ=255)

                                                                         tACGAGTTTGGTGAATCCCCCGCGGATAAGCGGCCCAGAAAGTGAGGATATAGATGTGGCAAAAACGGCAACACCGGAAGTAATGACCGAGATAGTGAACAATCGTGCTGATTCAGCAACAGGGACAATATCGCCATAGCCGa                                                                  <  2:408047/143‑1 (MQ=255)

                                                                          aCAAGTTTGTTGAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCATAGCCGAc                                                                 <  1:203159/143‑1 (MQ=255)

                                                                            aaGTTTGTTGAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGAGCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCATAGCCGACgg                                                               >  1:384513/1‑143 (MQ=255)

                                                                                 tgttgAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTGATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCATAGCCGACGGTTGAc                                                          <  1:904167/143‑1 (MQ=255)

                                                                                 tgttgAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTGATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCATAGCCGACGGTTGAc                                                          <  1:107382/143‑1 (MQ=255)

                                                                                   ttgAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCATAGCCGACGGTTGACAt                                                        >  2:160649/1‑143 (MQ=255)

                                                                                        tCCCCCGCGGATAAGCGGACCAATAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCATAGCCGACGGTTGACATGGttt                                                   <  1:123209/143‑1 (MQ=255)

                                                                                                         gACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCATAGCCGACGGTTGACATGGTTTCTATCGAAAAATAGAAc                                  >  1:716960/1‑143 (MQ=255)

                                                                                                            cAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCATAGCCGACGGTTGACATGGTTTCTATCGAAAAATAGAAcgcg                               <  2:716960/143‑1 (MQ=255)

                                                                                                              aaaaTTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCATAGCCGACGGTTGACATGGTTTCTATCGAAAAATAGAACGCGGt                             <  1:754731/143‑1 (MQ=255)

                                                                                                               aaaTTGAGGTCATAGATGTGGCAAAAACGATAATGCCGGAAATAATGACCGAAATAGTGAACAATCGGGCTGATTCAGAAACAGGGAAAATAACACCATAGACGACCGCTGACCTGCGTACTATCGAACAATAGAAcgcgacc                            >  2:670928/1‑140 (MQ=255)

                                                                                                               aaaTTGAGGTCAGAGATGTGGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCATAGCCGACGGTTGACATGGTTTCTATCGAAAAATAGAACGCGGtc                            <  1:815868/143‑1 (MQ=255)

                                                                                                                     aGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCATAGCCGACGGTTGACATGGTTTCTATCGAAAAATAGAACGCGGTCATCaaa                      <  2:255163/143‑1 (MQ=255)

                                                                                                                               tgtgGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCATAGCCGACGGTTGACATGGTTTCTATCGAAAAATAGAACGCGGTCCTCAAACTTTCTATTc            >  1:879447/1‑143 (MQ=255)

                                                                                                                                  ggCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCATAGCCGACGGTTGACATGGTTTCTATCGAAAAATAGAACGCGGTCATCAAACTTTCTATTcgcg         <  2:736288/143‑1 (MQ=255)

                                                                                                                                    cAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCATAGCCGACGGTTGACATGGTCTCTATCGAAGAATAGAACGCGGTCATCAAACTTTATATTACCGGa       >  2:712654/1‑143 (MQ=255)

                                                                                                                                         aCGGTAACGCCGGAAATAATGACAGCAACAGTGCACAATCGTGCTGCGTCAGAAACAGGAACAATATCGCCATAGCCGACGGTTGACATGGTTTCTATCGAATAATGGAACGCGGTCATCAAACTGTCTATTTGCGGATTaaa  <  2:451071/143‑1 (MQ=255)

TATTGATGGCGAGAATCGAATGTCCGCAAACAATAAAATGATCTTTACGATGCATTGTATGATTGTTTCCTTTTACAAGTTTGTTGAATCCCCCGCGGATAAGCGGACCAAAAATTGAGGTCATAGATGTGGCAAAAACGGTAATGCCGGAAATAATGACCGAAATAGTGAACAATCGTGCTGATTCAGAAACAGGGACAATATCGCCATAGCCGACGGTTGACATGGTTTCTATCGAAAAATAGAACGCGGTCATCAAACTTTCTATTCGCGGATTAAA  >  NC_000913/1309498‑1309777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`