Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,971,929 A→C 9.4% S255A (TCA→GCA)  tar ← methyl‑accepting chemotaxis protein Tar

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,971,9290AC 9.4% 102.4 / 3.4 49S255A (TCA→GCA) tarmethyl‑accepting chemotaxis protein Tar
Reads supporting (aligned to +/- strand):  ref base A (22/22);  new base C (2/3);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.82e-01

TTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTA  >  NC_000913/1971789‑1972064
                                                                                                                                            |                                                                                                                                       
ttcCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGCGCGTTGCATATGTGaaa                                                                                                                                       >  1:147864/1‑143 (MQ=255)
   cAGCGCGGATGCCTGCTGTTCAGTACGGGGGGAAAGATCGGTGGTGCGCGCCGCAATTTCAAGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGATACga                                                                                                                                    >  2:254724/1‑142 (MQ=255)
   cAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGACCCTTCGCGGACATGCGTGACGGTGGCAGGCAAAGACCGTTGCATATGGGACACGc                                                                                                                                    >  2:901502/1‑143 (MQ=255)
   cAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGc                                                                                                                                    >  2:901158/1‑143 (MQ=255)
   cAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGc                                                                                                                                    >  2:59948/1‑143 (MQ=255)
    cgcgcGGCTGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGct                                                                                                                                   <  2:135898/142‑1 (MQ=255)
    aGCGCGGATGCCGGCTGAGCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAAGTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGct                                                                                                                                   <  1:242305/143‑1 (MQ=255)
       gcgGTTGCCTGCTGTTCAGTTCGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGATCCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTTCACATATGCAACGctctt                                                                                                                                <  2:154085/143‑2 (MQ=255)
       gcgGCTGCCTGTTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCAGAGATGGCATCTGATCGTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTTCACATATGCAACGctctt                                                                                                                                <  2:154100/143‑2 (MQ=255)
       gcgGATGCCTGCTGTTCAGTAGGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTTCACATATGCAACGccctt                                                                                                                                <  2:295547/143‑2 (MQ=255)
                 gctgTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACAGGGGTAGATGGCATATGGAAAGACTAGTACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAGACGCTCTGCGCCAGGTCg                                                                                                                      >  1:914518/1‑143 (MQ=255)
                  ctgTTCAGTACGGGAGGAGAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTGCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGc                                                                                                                     <  2:665340/143‑1 (MQ=255)
                    gcgcAGCACGGGAGGACAGATCGGTGGTGCCCGCCGCAATGTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGccc                                                                                                                   <  2:336055/140‑1 (MQ=255)
                    gTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGccc                                                                                                                   >  2:875418/1‑143 (MQ=255)
                     ttCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCa                                                                                                                  >  1:407767/1‑143 (MQ=255)
                          tACGGGAGGAGAGAGCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCa                                                                                                             <  1:886847/143‑1 (MQ=255)
                          tACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCa                                                                                                             <  1:429301/143‑1 (MQ=255)
                                aggaAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTgcgc                                                                                                       <  2:765760/143‑1 (MQ=255)
                                      gATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCa                                                                                                 <  2:120740/143‑1 (MQ=255)
                                              ttGCCCGCCGCAATTTCACGGGTACCGGCATCGATGGCATCTGAACCTTCGCGGACCTGAGTGACGGTGTCAGTCGAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAg                                                                                         <  2:624058/143‑1 (MQ=255)
                                                gCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGCAACGCTCTGCGCCAGGTCGCCCATTTCACCGCGCCCGCCACTGGTCAcgg                                                                                       >  1:874563/1‑143 (MQ=255)
                                                  ccgccgCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAACCGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCACTGGTCAGGGTa                                                                                     >  1:898584/1‑143 (MQ=255)
                                                  ccgccgCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAACCGCTCTGCGCCAGGCCGCCCATGTCACTGCGCCCGTCAATGGTCAGGGTa                                                                                     >  2:545610/1‑143 (MQ=255)
                                                  ccgccgCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGACAAAGGGCAGGGTa                                                                                     >  2:224951/1‑143 (MQ=255)
                                                       gcAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGCGCGTTGCCTATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTCCCCCCGGCCATCGTCCGGGTATTCGc                                                                                >  1:218745/1‑143 (MQ=255)
                                                           tttCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGGCAAAGCGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAAGGGTCAGGGTATTCGCCAgg                                                                            >  1:300052/1‑143 (MQ=255)
                                                              cccGGGTATCGGCATAGAGGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTa                                                                         <  1:401655/141‑1 (MQ=255)
                                                                 gggTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGGCAAAGAGCGTTGCATATGTGCAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCGGTCAATGGTCCGGGTATTCGCCAGGTTaaca                                                                      >  1:13184/1‑143 (MQ=255)
                                                                  ggTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGACCGTCAATGGTCAGGGTATTCGCCAGGATaccac                                                                     >  1:329422/1‑143 (MQ=255)
                                                                        ggCATAGATGGCATCTGAACCTTCGCGGACATGAATGAAGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGACCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCAGcgacga                                                               >  1:573142/1‑143 (MQ=255)
                                                                           aTAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTCGCATATGTGAAACGCTGTGCGCCAGGTCGCCCATTTCACTGCGGCCGTCAATGGTCAGACTATTCGCCCGCGTACCAACGGCGAttc                                                            >  2:578162/1‑142 (MQ=255)
                                                                             tgacggCATCTGAACCTTCGCGGACGTGGGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTGCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTcg                                                          <  2:6856/139‑1 (MQ=255)
                                                                                      cTGATCCTTCGCGGACATGAGTGACGGGGGCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGa                                                 <  1:700181/143‑1 (MQ=255)
                                                                                      cTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCTATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGa                                                 <  1:221585/143‑1 (MQ=255)
                                                                                      cTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCAGCGGCGATGTCGCGCATGTGa                                                 >  2:686137/1‑143 (MQ=255)
                                                                                      cTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGa                                                 <  1:810978/143‑1 (MQ=255)
                                                                                       tGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTCACCACCGGCGATTTCGCGAAtgcgcg                                                >  2:273480/1‑139 (MQ=255)
                                                                                              tCGCGGCCCTGGGTGACGGTGCCAGTCAAAGAGCGTGGCATATGTGAACCGCGCTGCGCCAGGTCGCCCATTTCGCTGCGCCCGTGAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCaataat                                         <  2:433950/143‑1 (MQ=255)
                                                                                              tCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCaataat                                         <  2:669044/143‑1 (MQ=255)
                                                                                                 cgGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTTAAACGCGCTTCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAAtttt                                      >  1:629601/1‑143 (MQ=255)
                                                                                                          gTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGCATGTTAGCAATAATTTTTGCGAGCgg                             >  1:49958/1‑143 (MQ=255)
                                                                                                           tGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGa                            <  2:703605/143‑1 (MQ=255)
                                                                                                            gACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAg                           <  2:443899/143‑1 (MQ=255)
                                                                                                                 tgtCAGTCAAAGAGCGTTGCATATGCGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATTTGAGCAATAATTTTTGCCAGCGGAGTAAGc                      <  1:244505/143‑1 (MQ=255)
                                                                                                                  gtcagtcaAAGCGCGCTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATGTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCa                     <  2:885467/143‑1 (MQ=255)
                                                                                                                   tcggtgcaaGGGCGTTGGCTATTTGAAACGCTCTGCGCCAGGTCGCCCATTTCACCGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCaa                    <  2:704913/136‑1 (MQ=255)
                                                                                                                          aaGAGCGTGGCATATGTGAAACGCGCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACgg             <  1:47890/143‑1 (MQ=255)
                                                                                                                                gTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTACGCAACATACGGCGAATg       >  1:718572/1‑143 (MQ=255)
                                                                                                                                gTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATg       >  1:725018/1‑143 (MQ=255)
                                                                                                                                     atatGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATGCGCCAGGGTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCGACATACGGCGAAGGCCGTa  >  1:70134/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                       
TTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTA  >  NC_000913/1971789‑1972064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑