| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,387 | C→T | 100% | Q791* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,387 | 0 | C | T | 93.7% | 105.4 / ‑4.5 | 46 | Q791* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (1/1); major base T (20/19); minor base G (2/2); total (24/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
GATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAAC > NC_000913/125248‑125527 | gaTCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAgg < 1:895592/143‑1 (MQ=255) tGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAAATACGCGCGTTCCGGATATCCCTTAGGggatg > 2:187049/1‑143 (MQ=255) ggCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTAAGGATTGTGAACGCTGGAACATGCTGCACCCGCCGGAAACTCCGCGCGTTCCGGATATCACGCAGGtgaggg > 2:107766/1‑140 (MQ=255) gCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTGAGGCGATGaa > 2:637917/1‑143 (MQ=255) cGAAAGATTACGGCGGAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAac < 1:633190/143‑1 (MQ=255) cGGCGTAGGTTCTGACGGTTCTAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCgg < 2:248594/143‑1 (MQ=255) tAGGTTCTGACGTTTATAGCGTGACCTCCTTCCCCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTg < 1:783539/143‑1 (MQ=255) gTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGCGGCa > 2:137116/1‑143 (MQ=255) gTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGa < 1:731902/143‑1 (MQ=255) aGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTatat < 1:114878/143‑1 (MQ=255) cGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGa > 1:880061/1‑143 (MQ=255) gcacttgctTCACCGGGCGGGCGCGTGATGGCCAGGATTGTGAACGCTGGAACATGCTGCCCCCGCTGTAAACTCCGCGCGTTCCGTCTATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGaaa < 2:771284/136‑1 (MQ=255) aCCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACACGCTGGAAACTCCGCGCGTTCCGTAGATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCGTCTACCGACTCTATGAAACt > 1:703457/1‑143 (MQ=255) tcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGACCGCCGCTCCGTCATGGGCATCTACCGACTATATGAAACTGtt > 1:620690/1‑143 (MQ=255) ctTCCCCGAGCTGGAGCGTGACGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCg < 2:220808/143‑1 (MQ=255) ctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAGCTGTTCg > 1:368685/1‑143 (MQ=255) ctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTAGATGAAACTGTTCg > 2:301336/1‑143 (MQ=255) cGAGGTGGCGCGGGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGc < 1:127908/143‑1 (MQ=255) cttgggcgTGCAGGCCAGGAGTGTGGACGCTGGAACATGCTGCACCCGCTGGAACCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGt < 1:861633/138‑1 (MQ=255) ggCGCGTGATGCTCAGGATTGCGAACGCTGGAACATGCTGCACCCGCTGGAAACGCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTcc < 1:610289/143‑1 (MQ=255) cgcgTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGt < 1:770748/143‑1 (MQ=255) gATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTCGGCGATTAACGTCGCTCCGGCAGACGCAACTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGGACttg > 1:468730/1‑142 (MQ=255) tCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTCCGTAc > 1:390632/1‑143 (MQ=255) tgtgAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCTGTATATCGCTTAGGTGATGAACGACGTTCCGGCAGTGGTAGCTACCGACTATATGAAACGGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTga < 1:742489/143‑1 (MQ=255) gtgAACGCTGGAACATGCTGCACGCGCTGGAAACTCCGCGCGTTCCGTATATCGCATAGGTGATGACCGACGCTCCGGCAGTGGCATCCACCGCCTATATGAAACTGTTCGCTGCGCAGGTCCGTACTTACGTACCTGCAgac > 2:438521/1‑143 (MQ=255) tgAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGAGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAAATGTTCGCTGAGCAGGTCCGTACTTACGGACCGGGTgacg > 1:99866/1‑143 (MQ=255) gAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAAGGACGGTCAGGCAGTGGCATCTACCGACTATTTGAAACGGTGCGGTGAGCAGGTCCGTAGTTACGTACCGGCTgcgga > 1:170846/1‑139 (MQ=255) cGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTa > 1:387822/1‑143 (MQ=255) gCTGGAACAATCTCCACCCGCTTGAAACTCTCCGCGTCCCGTATATCGCTTAGGTGACGAACGACGCTCAGGCAGTGGCATCCACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTAc < 2:53547/143‑1 (MQ=255) gAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACcgcg > 2:470011/1‑143 (MQ=255) gAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTAAGGTGCTGAACGACGCCGCGGCAGGGGCATGTACCGGCTATGTGGAACTGTTCGCTGGGCGGGGCCGCACTTACGTACCGGCCGACGACCACcgcg > 2:628264/1‑143 (MQ=255) gAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCCGAGGTGATGAACGACGCTCCGGCCGTGGCATCTACCGACTATCTGAACCTGTTCGCCAAGCCGGTCCGTACTAACCTACCGGACCACGACTGCcgcg > 2:789558/1‑143 (MQ=255) ctctgaccccGCTCGAAGCGCCGTGCGGTCCGTACATCGCTGATGTGATGAACGACGCTCCGGCAGTGGTATCTACCGACTATATGAAGCTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTg < 2:224501/136‑1 (MQ=255) gctccACCCGGTGGAAGCTCCGTGCGTCCCTTACATCGCTTAGGTGTTGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAATCTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTgg < 2:423078/143‑1 (MQ=255) cACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTAc > 2:315503/1‑143 (MQ=255) cACCCGCGGGAACCTCCGCGCGTTCCGTATATCGCGTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTAc < 1:187755/143‑1 (MQ=255) tCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTGCTGGGTACTGATGGCTTCGGTc > 1:461573/1‑143 (MQ=255) cgcgcgTTCCGTATATCGCTTAGGTGATGACCGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGt < 1:137116/143‑1 (MQ=255) cgTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCg < 1:637917/143‑1 (MQ=255) cGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGCAACTGTTCGCTGAGCGATTCCGTATTTAGGTACGCGCTGACGGGAACCGCGTACTGGGTACTGATGGCTTCGGTCGTTGCGACAgg > 1:716136/1‑142 (MQ=255) gTAGATCGCGGAGGTCATGAACGACGATGCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGcc < 1:597403/143‑1 (MQ=255) tatCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTg < 2:628073/143‑1 (MQ=255) tatCGCTTAGGTGATGAACGACGCTCCGGCAGCGGCATCCACCGACTGTATGAAACTGTTCGCCGAGCGGGTGCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACGGACGGCTTCGGGCGTCCCGACAGCGGTg > 2:243393/1‑143 (MQ=255) cGCTTAGGTGATGAACGACGCTGCGGCAGTGGCATCGACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACGGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGCGAGGGGTgaga > 1:505513/1‑143 (MQ=255) cGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTAGTGATTATGTGAGACTGTGCGCGGAGAGGGTCAGTTTGCACCCACCGGCTGACGACTACCGCGTACTGGCCACTGACGGCTTCCGTCGTTCCGACAGCCGCgaga > 1:673431/1‑143 (MQ=255) cTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAAc < 1:301336/143‑1 (MQ=255) | GATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAAC > NC_000913/125248‑125527 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |