Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,661,969	C→G	100%	V55L (GTT→CTT)	iscR ←	DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,661,969	0	C	G	100.0%	217.7 / NA	68	V55L (GTT→CTT)	iscR	DNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (40/28);  total (40/28)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATA  >  NC_000913/2661832‑2662107                                                                                                                                           |                                                                                                                                           gccCTGGCTGCCGCCTTTACGCTGGCAACGGGTGGCATCTACAGGTTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGAc                                                                                                                                       <  2:560711/143‑1 (MQ=255) gccCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGAc                                                                                                                                       <  1:455143/143‑1 (MQ=255)  cccTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGAcc                                                                                                                                      <  2:833815/143‑1 (MQ=255)         aGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTa                                                                                                                               <  2:717738/143‑1 (MQ=255)          gcggccTTAACCCCGACAAGGGGTGGCATCTCCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTAc                                                                                                                              <  2:5646/140‑1 (MQ=255)          cgcgccTTTACCTTTACTACCGATGGCAGCTACAGATTCGTCAACGGCGCTCTTTACTTCCCCAACGGCGATGCTGCTGG‑CATCTTTGTCTAACAAAAATTCTATGTTTGTTGTACGTACGCTGGAAAGCAGACCATTTTTAc                                                                                                                              <  2:750151/141‑1 (MQ=255)           ccgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGTCCTC‑TGCCCGCTCATATAACCACCGCCTGGTCCACGTCCGCTGGGAAGCAGACCATTTTTACg                                                                                                                             >  1:422724/1‑143 (MQ=255)           ccgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACg                                                                                                                             >  1:44498/1‑143 (MQ=255)           ccgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCCGG‑CATCTTTGCCTAACAGATAACCACCGCCTGCTCCACGTAAGCAGCAACGCAGACCATTTTTCCg                                                                                                                             >  2:358153/1‑143 (MQ=255)            cgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGc                                                                                                                            >  2:31053/1‑143 (MQ=255)              ccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAg                                                                                                                          >  1:38305/1‑143 (MQ=255)                 tgaCCCTGACAACGGGTGGCAGCTACAGATTCGTCAACGGCGCTGATTACTTCGCCAACGGCGATGCTGCGGG‑CCTCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACg                                                                                                                       <  1:654705/141‑1 (MQ=255)                     ccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACgggga                                                                                                                   >  2:505218/1‑141 (MQ=255)                     ccTGACAACGGGTGGCAGCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGaa                                                                                                                   <  2:161479/143‑1 (MQ=255)                      cTGACACCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGaaa                                                                                                                  <  1:162132/143‑1 (MQ=255)                       tGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCCGACCCTTTTTACGCAGACGGGaaaa                                                                                                                 >  1:338417/1‑143 (MQ=255)                         aCAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACa                                                                                                               <  2:277731/143‑1 (MQ=255)                          cAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAg                                                                                                              >  1:539180/1‑143 (MQ=255)                              gggTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAgttgt                                                                                                          <  2:659969/143‑1 (MQ=255)                                 tGGCATCGACAGATTCGTCAACGGCGCTAATTACTTCGTCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTcc                                                                                                       <  2:123450/143‑1 (MQ=255)                                    cATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAgc                                                                                                    >  1:750799/1‑142 (MQ=255)                                           ggcttCGTCAACGGCCCTAATTACTTCGCCAACGGCGATGCTGCGGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGaaa                                                                                             <  1:411309/140‑1 (MQ=255)                                           agcttCGTCCACGGCGCTAATTACGTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGaaa                                                                                             <  2:27613/140‑1 (MQ=255)                                           aGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGaaa                                                                                             >  1:405628/1‑143 (MQ=255)                                           aGATTCCTCACCGGCGCTAAGTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGaaa                                                                                             <  1:336449/143‑1 (MQ=255)                                             atgcgccAACGGCGCTAATTACCTCACCAACCACGCACCTGGTGG‑CATCTCTGCGGAACACAGAACCACCGACTGATCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAgg                                                                                           <  1:422449/137‑1 (MQ=255)                                             aTTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGGAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAgg                                                                                           >  1:18902/1‑143 (MQ=255)                                             aTTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAgg                                                                                           <  1:581722/143‑1 (MQ=255)                                                 gacAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGaaa                                                                                       >  1:321106/3‑143 (MQ=255)                                                  tccaCGGCGCGAATTGCTTCGCCCAGGGCGAGGGTGCTGG‑CGTCTTTGCCTAACCGATGACCACCGCCTGGGCCACGTACGCTGGAAAGCATACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAAt                                                                                      <  2:513448/140‑1 (MQ=255)                                                  tCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGACAAGAAAGGGAAAt                                                                                      >  2:134003/1‑143 (MQ=255)                                                  tCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTCTTACGCAGACGGGAAAACAGCTGTTCCAGATAAGACAGGGAAAt                                                                                      >  2:354227/1‑143 (MQ=255)                                                                   ttCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGTAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGaaa                                                                     >  2:619506/1‑143 (MQ=255)                                                                   ttCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa                                                                     >  1:600294/1‑143 (MQ=255)                                                                   ttCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa                                                                     >  1:253510/1‑143 (MQ=255)                                                                      gCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATGCCCTGACGTACGGAAatat                                                                  >  2:601696/1‑143 (MQ=255)                                                                       ccAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTCCGGAAATATc                                                                 >  1:138157/1‑143 (MQ=255)                                                                         aaCGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAg                                                               >  1:544188/1‑143 (MQ=255)                                                                            ggCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCa                                                            >  1:337725/1‑143 (MQ=255)                                                                            ggCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCa                                                            >  1:337765/1‑143 (MQ=255)                                                                               gATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACg                                                         >  2:730530/1‑143 (MQ=255)                                                                                    ggctgG‑CAGCTTCGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTAcc                                                    <  1:637151/142‑1 (MQ=255)                                                                                      ctgG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATATGGCAGCAAAAATCCCTGACGTTAGGAACTGTCAGCCAACGGTACCgg                                                  >  1:907444/1‑143 (MQ=255)                                                                                       tgG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCggg                                                 >  1:549932/1‑143 (MQ=255)                                                                                             tCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGc                                            <  2:44498/143‑1 (MQ=255)                                                                                               tttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGGTGTGCCAGATAAGAAAGGAAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTGCCGGGGCCGCtt                                          >  2:511612/1‑143 (MQ=255)                                                                                                 ggCCCAACGGACAACCACCGCCCGGGCGACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTGGCTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCa                                        <  1:50795/142‑1 (MQ=255)                                                                                                   ccTAGCAGATAACGACCGCCTGGTCCACGTACGCTGTAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCaga                                      <  1:901182/143‑1 (MQ=255)                                                                                                   ccTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCaga                                      <  1:789710/143‑1 (MQ=255)                                                                                                     tAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTGCGGGGCCCGCTTCAGAGt                                    >  1:813898/1‑143 (MQ=255)                                                                                                        cAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTACCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGa                                 <  1:917136/143‑1 (MQ=255)                                                                                                         aGATAACCACCGCCTGGTCCACGTACGCTGGAAAGGAGACAAATTTT‑CGTGGCCCGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgc                               <  1:208136/143‑1 (MQ=255)                                                                                                         aGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAACGGGAAATTCCCTGACGTTCGGAGATCTCAGCCAACGGTACCGGGCCCGCTTCCGAGTTCAg                                >  1:737699/1‑143 (MQ=255)                                                                                                          gctcaccaccGCCTCGTCCACGTACGCTGGAAAGCAGACCATGTGTGCGGAGGCGGGAAAACAGTTGGACCAGATACTGAAGGGAAATTCCCTGGCGTTCGGAAATATCGGCGAACGGTACCGGGCCCGCTTCAGAGTTGAgc                               <  2:409415/139‑1 (MQ=255)                                                                                                           aTAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGGTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCGTCAGAGGTGAgcg                              >  2:453722/1‑143 (MQ=255)                                                                                                              accaccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGGAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCaa                           <  1:505218/143‑1 (MQ=255)                                                                                                               ccaccGCCTGGTCCACGTACGCTGGAAAGCGGACCATTTTTACGCCGACGGGAAAACAGTTGTTCCAGATAGGAAAGGGAAATTCCCTGACGTTCGGCAATATCCGCAAACGGTACCGGGCCCGATTCAGAGCTGAGGGCGAc                          >  1:811537/1‑143 (MQ=255)                                                                                                                    gCCTGGTCCACCTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACATTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTAGAAACTATCATCCAATGGTACCGTGCCCGCTTCAGAGTTGAGCGCAACGTCaa                     <  1:254295/143‑1 (MQ=255)                                                                                                                     ccTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGGTGAGCGCAACGTCAAg                    >  2:585896/1‑143 (MQ=255)                                                                                                                         gTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCAtt                >  2:89426/1‑143 (MQ=255)                                                                                                                          tCCACGTACGCTGGAAAGCAGACCATTTTTATGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTg               >  1:24290/1‑143 (MQ=255)                                                                                                                          tCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAATGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCACCGTCAAGCAtcg               >  2:604014/1‑141 (MQ=255)                                                                                                                          tCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTg               >  1:659805/1‑143 (MQ=255)                                                                                                                          tCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTg               >  1:226607/1‑143 (MQ=255)                                                                                                                                tACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCa         >  2:212697/1‑143 (MQ=255)                                                                                                                                tACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGCTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCCACGGCACCGGGCCCGCTTCCGAGCTGAGCGCAACGTCCCGCCTTGCGGCCa         >  2:907886/1‑143 (MQ=255)                                                                                                                                   gCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACgg      >  2:827428/1‑143 (MQ=255)                                                                                                                                       gAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGTGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGACATa  >  2:794964/1‑143 (MQ=255)                                                                                                                                           |                                                                                                                                           GCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATA  >  NC_000913/2661832‑2662107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑