Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,041,632	T→A	100%	V107E (GTG→GAG)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,632	0	T	A	100.0%	147.8 / NA	50	V107E (GTG→GAG)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (27/23);  total (27/23)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGG  >  NC_000913/3041493‑3041771                                                                                                                                            |                                                                                                                                            gccCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGAc                                                                                                                                          <  1:142174/143‑1 (MQ=255)           aCGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACCGAAAAAATGAGCGGTATTCTCTAAAGAGACCATCGCGCCa                                                                                                                                >  2:448074/1‑143 (MQ=255)             gCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAGATATGCGGTATTCTCTAAAGAGACCATCGCGCCAga                                                                                                                              >  2:252448/1‑143 (MQ=255)                aaaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAGCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAgacga                                                                                                                           >  2:68779/1‑143 (MQ=255)                        gaTGTGGAGCAAGTTACGTCTGTTCCGCGACGGCGATGGCTGTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCGGACTGAACTGAAAAAATATGCGGTATCCTCTAAAGAGACCATCGCGCCAGACGACGAGCgtg                                                                                                                   <  1:39155/142‑1 (MQ=255)                        aaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCgtg                                                                                                                   <  1:259319/143‑1 (MQ=255)                        aaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCgtg                                                                                                                   <  2:36557/143‑1 (MQ=255)                          tgtgGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGtgtg                                                                                                                 <  1:137772/143‑1 (MQ=255)                          ggtgGGGCAGTTTACGTCTGTTCCGCGACGGCGAGGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGtgtg                                                                                                                 <  2:302161/142‑1 (MQ=255)                            tgGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGCCTGAACTGAAAAAATATGCGGTATTCTCTAACGAGACAACCGCGCCAGACAACGGGCCTGtgct                                                                                                               >  2:626586/1‑143 (MQ=255)                                 cAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                                                                                          >  1:861956/1‑143 (MQ=255)                                 cAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                                                                                          <  1:534735/143‑1 (MQ=255)                                   atgtACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAGCGGGGGGGCGCCCGACAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgt                                                                                                        <  1:422055/140‑1 (MQ=255)                                    tttACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGCGTGCTGCTTGgtg                                                                                                       >  1:403116/1‑143 (MQ=255)                                    tttACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACCGAAAAAATATGCGGTATTCTCTAAAGAGCCCATCGCGCCACACGACGAGCGTGTGCTGCTTGgtg                                                                                                       >  2:739215/1‑143 (MQ=255)                                     ttACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCTGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGCCCATCGCGCCAGACGACGAGCGTGTGCCGCTTGgtgt                                                                                                      >  2:417530/1‑143 (MQ=255)                                          tCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGGGTTGCCg                                                                                                 >  1:6399/1‑143 (MQ=255)                                          tCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTACTGCTTGGTGTCGCCg                                                                                                 >  2:737490/1‑143 (MQ=255)                                                   cgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAgg                                                                                        <  2:914349/143‑1 (MQ=255)                                                   cgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAgg                                                                                        >  1:602568/1‑143 (MQ=255)                                                    gACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgc                                                                                       <  1:842419/143‑1 (MQ=255)                                                     aCGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcg                                                                                      <  2:382895/143‑1 (MQ=255)                                                                      gggATTGAACGGCGCCGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaaa                                                                     <  2:350984/142‑1 (MQ=255)                                                                         aTTGACCGGCCCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct                                                                  <  1:304912/143‑1 (MQ=255)                                                                         aTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCCGGCGCGCGCCGCGCCGGCaacgct                                                                  >  2:714032/1‑139 (MQ=255)                                                                         aTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct                                                                  >  2:686121/1‑143 (MQ=255)                                                                         aTTGAACGGCGCAGCGTGCGAGAACCGCAGCTTACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGGAAAtct                                                                  >  2:477959/1‑143 (MQ=255)                                                                              aCGGCGCAGCGTGCGGGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCCCTTTa                                                             >  2:386365/1‑143 (MQ=255)                                                                               cGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCtttgg                                                            <  1:9689/143‑3 (MQ=255)                                                                                ggCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTCGc                                                           >  1:23943/1‑143 (MQ=255)                                                                                         cgcgttAACCGCATCTAACTTAACTCAACAACTATGCGGCATTCTCTAAAGAGACCATCGCGCCAGACGACGGGCGTGTGCTGCTTTGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCt                                                  <  2:413121/142‑1 (MQ=255)                                                                                             tgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCGAATCTCTTTAGCGAACTGCCTTCgc                                              >  1:116908/1‑142 (MQ=255)                                                                                                aCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTGGCGGACTGCCTTCgaaag                                           >  2:716834/1‑143 (MQ=255)                                                                                                 ccGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCGAATCTCTTTATCGCGCTGCCTTCggaagg                                          >  1:608698/1‑142 (MQ=255)                                                                                                    cAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGCGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAGAGaaaa                                       >  1:14709/1‑143 (MQ=255)                                                                                                     aGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGACCTGCCTTCGAAAGaaaaa                                      >  2:639804/1‑143 (MQ=255)                                                                                                      gCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAAc                                     >  1:146899/1‑143 (MQ=255)                                                                                                        ggactcaGCTGAAAAAATATGCGGTATTCTCTAAACAGACCATCGAGACAGACGACGAGCGTGTGCTGTCTGGTGTTGCCGGTTTTAAGGCGCGAGACGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAg                                   <  2:517023/142‑1 (MQ=255)                                                                                                         gcctgaactgaaGAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGGTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAgg                                  <  2:582430/141‑1 (MQ=255)                                                                                                              aactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGCACTGCCTTCGCAAGAAAAACAGGTAggc                             >  2:341192/1‑141 (MQ=255)                                                                                                              aactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTGTCAGGCGCGCGCCGCGCTGGCCAATCTCTTTCGCGAACTGCCTTCGAAGGAAAAACAggccgtc                             >  1:182798/1‑138 (MQ=255)                                                                                                                  gaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaag                         >  1:492248/1‑143 (MQ=255)                                                                                                                       aaTATGCGTTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgc                    <  2:157165/143‑1 (MQ=255)                                                                                                                           ggCGGTAGTCGCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGa                <  2:715179/142‑1 (MQ=255)                                                                                                                            gCGGTATTCTTTAAAGAGTACATGGCGGCAGACGGCGAGGGTGAGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGAc               <  1:9808/143‑1 (MQ=255)                                                                                                                                  ttCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACttt         >  1:585082/1‑143 (MQ=255)                                                                                                                                   tctcTAAAGAGACCATCGCCCCCGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTCGCAAATCTCTTTAGCGAACTGCCTTCGACAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTg        <  1:518746/143‑1 (MQ=255)                                                                                                                                   tctcTAAAGAGACCAGCGCGCCAGGCGACGTGCGGGTGGTGCTTGGTGTTGCGGGTTTTCGGTCTCGCGTCGAGATGGCAAATCTCTTTAGCGAACCGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTg        <  2:795722/143‑1 (MQ=255)                                                                                                                                     tctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCt      >  1:102431/1‑143 (MQ=255)                                                                                                                                         aaGAGACCATCGCGCCAGACGACGAGCGGGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATgg  <  1:272098/143‑1 (MQ=255)                                                                                                                                            |                                                                                                                                            GCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGG  >  NC_000913/3041493‑3041771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑