| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,332,822 | G→A | 11.2% | intergenic (‑87/+40) | yhbE ← / ← rpmA | inner membrane protein YhbE/50S ribosomal subunit protein L27 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,332,822 | 0 | G | A | 11.2% | 101.2 / 6.5 | 45 | intergenic (‑87/+40) | yhbE/rpmA | inner membrane protein YhbE/50S ribosomal subunit protein L27 |
| Reads supporting (aligned to +/- strand): ref base G (23/17); new base A (2/3); total (25/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.01e-01 | |||||||||||
GCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACG > NC_000913/3332689‑3332957 | gCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACACGTTGCggg < 1:729027/143‑1 (MQ=255)gCTGGGGTGAGCGCCAAAAGAATTCTAAGGCCTGGCTGCGGCTTCATACCGTATTCCCTGCAAAGCGCTTTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACACGTTGCggg < 2:165616/143‑1 (MQ=255) cTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACACGTTGCgggg < 2:344247/143‑1 (MQ=255) tgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCa > 2:769037/1‑143 (MQ=255) gAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCAt < 2:845880/143‑1 (MQ=255) aaaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGGGTTGCGGGGCTTTCATCCGTTAc > 1:506706/1‑143 (MQ=255) atgccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCATAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGAc < 2:226456/143‑1 (MQ=255) tgccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCCTTTTTACCGGTTATCGAACGTAAAAAGCCCCGCAAAGGTTCGCCGGGCGTTCATCCGTTACCGGGAcg > 2:8723/1‑143 (MQ=255) caatgccTGCCTGCTGTTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGa > 2:423977/1‑143 (MQ=255) ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACt > 2:55863/1‑143 (MQ=255) ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCAGTTATCGCATGTAAAAACCCCCGCAACGTGTTGCGGGGCTTGCATCCGCTACCTGGAcgcgcagacct > 2:495100/1‑136 (MQ=255) tgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACtt > 2:467975/1‑143 (MQ=255) ctgctgCTTCATACCGTATTCCCTGCAAAGCGCATTCGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTAtt < 1:41515/143‑1 (MQ=255) tgctTCATACCGTGTTCCCTGCAAAGCGCAGTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGc > 1:897669/1‑143 (MQ=255) tgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGTTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATACTGTACCTGGACGCGAGAAAATTCTTCAGc > 2:533465/1‑143 (MQ=255) ctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTAACCGCGACGCGCACAACTTACACAGCtt > 2:319535/1‑143 (MQ=255) aCCGTATTCCCTGCAATGCGCATTTGCGGCTTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGc < 2:280297/143‑1 (MQ=255) gcctcTGCAAAGCGCATTTGCGGACTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATaa < 1:292940/139‑1 (MQ=255) cTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAAttt > 2:467616/1‑143 (MQ=255) cTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGCGCTGCGGGGCGTTCATCCGTTCCCGCGGCGCCACAAACTTACTCAGCTACGCTGCTGATgaaatc > 2:73144/1‑140 (MQ=255) ttaaaGCTCAATTGCGGAGTAACTTCGCACCATGCGCGTTTTTACTGGTTATCTAATGTAAAAAGCGCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTAc < 2:118372/141‑1 (MQ=255) gCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTAc < 2:208551/143‑1 (MQ=255) aTTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCg > 1:378149/1‑143 (MQ=255) tttGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCgg > 1:800371/1‑143 (MQ=255) ttAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTACATGTGGATAAATTTACGGTTTTTCGGGCCTTTaa < 1:474383/143‑1 (MQ=255) gtgaCGCCGCGCCATGCGTATTGGTACCGGTTAGCGAATGGACAAAGCGCCGCAACGTGTTGCGTGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTaa < 2:343961/140‑1 (MQ=255) gTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCg > 1:748370/1‑143 (MQ=255) cGCACCGTGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGaa < 1:274892/143‑1 (MQ=255) cGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGaa < 1:498586/143‑1 (MQ=255) gCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAAt > 1:312639/1‑143 (MQ=255) aCCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGCTCAATTTCCGGTTTTTCGTGCTTTTAACTTCGAAttt > 1:363395/1‑143 (MQ=255) aTGCGCATTTTTACCGGTTATCGAATGCAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCAc < 2:202996/143‑1 (MQ=255) aTGCGCATATCAACCGGATAACCAACGTACTAACCCCCGCAACGTTTGGCGGCGCTATCATCCGATACCGGGACGCGAAAAACGTATTCAGCTTCGATGCTGATAAATGTACGGGATTGCGGGCCTTCAACTTCGAATTacac > 2:199524/1‑143 (MQ=255) gcATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTGCGGTTCTTCGGGCCTTTATCTTTGACTTTCCCTTTa > 1:30117/1‑143 (MQ=255) aTTTTGACCGGTGATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTAcc < 1:898172/143‑1 (MQ=255) aCCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGc < 2:638627/143‑1 (MQ=255) gTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTa > 2:230923/1‑143 (MQ=255) cGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCaaa < 1:423977/143‑1 (MQ=255) cGAACGTAAAAAGCCCCGCCACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCaaa < 1:475548/143‑1 (MQ=255) ccccGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGCACAACTTATTCAGCTTCGATGCTGATAAATTTACGGTGTTTCGGGCCTTTAACTTCGAATTTCACTTTCCCGTCTGCTTTGGCAAACAGAGTGTGGTCa > 1:196859/1‑143 (MQ=255) ccccGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCa > 1:195121/1‑143 (MQ=255) ccccGCAACGTGTTGCGGGGCTCTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGTCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCa < 1:131988/143‑1 (MQ=255) cccGCAACACGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGGGTGGTCAc > 1:514737/1‑143 (MQ=255) cccGCAACACGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAATGTGGTCAc > 2:471665/1‑143 (MQ=255) ccGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGACAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCGTTTAACTTCGCATTGCACTTTACCGCCTGCTTTAGCAAACAGCGAGTGCTCACg > 2:112221/1‑143 (MQ=255) | GCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACG > NC_000913/3332689‑3332957 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |