| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,911,819 | T→G | 9.1% | intergenic (‑294/+20) | pstS ← / ← glmS | phosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,911,819 | 0 | T | G | 9.1% | 114.2 / 2.3 | 43 | intergenic (‑294/+20) | pstS/glmS | phosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (14/24); new base G (2/3); total (16/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
TCAATTACAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCAC > NC_000913/3911677‑3911958 | tCAATTACAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCt > 2:73494/1‑143 (MQ=255) cAAACATTAATAACGAAGAGATGACGAACAAATTTACATTCTGTGGTAGAGACATAGTAGGTGAACTCGGTGGATTGTCACATGGAGTTGGCAGGATGTTTGACAGAAATTGTGTCAGGAAGAAAAATGCCCCGCTTACGCAg < 2:449426/143‑1 (MQ=255) aaaCATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAgg > 2:652973/1‑143 (MQ=255) aaaCATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAgg < 1:265960/143‑1 (MQ=255) taaCGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCTGCGTAAGCGGGGCAtttttc < 2:855013/143‑6 (MQ=255) aaCGAAGAGACGACAGAAAAATTTTCATGCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCAttt < 2:501789/143‑1 (MQ=255) ggAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATttat < 1:677518/142‑1 (MQ=255) aaGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATttatta < 2:464602/143‑1 (MQ=255) agggaggACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTGTGATTAACAACATAACAGGAAGAAAAATGCCCCGCTTAGGCAGGGCATCCATTTATTAc < 2:479310/137‑1 (MQ=255) gagaTGACAGAAAAATTTTCAGTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTTACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACt < 2:561961/143‑1 (MQ=255) aagACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATAACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCaa < 1:500899/141‑1 (MQ=255) aaaaaTTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAGACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTCTTACTCAACCGTAAc > 1:669628/1‑143 (MQ=255) aaaTTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCg > 1:36627/1‑143 (MQ=255) gtgtCATTCTCTGACAGGTGAAAAGTAGCCGAAGATGACGGTTTGTCACAGGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGAtt < 2:910467/140‑1 (MQ=255) tCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAg < 1:226644/143‑1 (MQ=255) tgtgACAGAGAAGAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGt < 2:299937/143‑1 (MQ=255) cAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTAcgc < 1:440080/143‑1 (MQ=255) agagAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACAGATTTTACCAGATTAcaag > 1:806976/1‑140 (MQ=255) gaAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCt < 2:769851/143‑1 (MQ=255) gaAAAAGAAGCCGAAGATTACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCt < 2:528150/143‑1 (MQ=255) aaaaaGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGCTCTTGCCAGGTTACGCGGCTg > 2:421075/1‑143 (MQ=255) gTAGCCGACGATGGCGGTCTGTCACATGGAGTTGGCAGCGTGTTTGATTAAAAACATAACAGGAACAAATAGGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCaa < 2:465443/143‑1 (MQ=255) tAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCATGTCTTCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAAc < 1:823748/143‑1 (MQ=255) cggcggtGACGGTTTGTCGCATGGAGTGGGGGGGGTGTGTGGTGAAGGGCGTAACGGGAAGAAAAAGGCCCGGCTTGCGCAGGGCATCCATTTATTACTCGACCGTAGCCGGTTTTGCCAGGTTACGCGGCTGGTCAACGTCg < 2:244295/137‑1 (MQ=255) cGAAGGTGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCACCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCg < 2:628430/143‑1 (MQ=255) gAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATCTATTACTCAACCGTAACCGATTTTGCCCGGTTACGCGGCTGGTCAACGTCgg > 2:547801/1‑143 (MQ=255) gTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGAt > 1:656306/1‑143 (MQ=255) tGTCACAGGAAGTTGCCAGGAGGTTTGATAAAAAACATACCAGGAGGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAg < 1:891057/143‑1 (MQ=255) aTGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGAc > 1:5888/1‑143 (MQ=255) tGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACa > 2:754319/1‑143 (MQ=255) cAGAATGTTTGAGTAAAAACATAACAGGAAGCAAAATGCCCCGCGTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGcc < 1:228352/143‑1 (MQ=255) aGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCca > 2:535027/1‑143 (MQ=255) ttAAAAACATAACAGGAAGAGACATGCCCCGCTTACGCAGGGCATCCATTTATGACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTATGCCAGCAGCTGCAGc < 2:774904/143‑1 (MQ=255) aaaaCATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTCAGCCACCAGCTGCAGCgca > 1:788467/1‑141 (MQ=255) aGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGgaga > 1:228356/1‑143 (MQ=255) ggAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGGCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGCACGGTGTagaa > 1:128673/1‑143 (MQ=255) ggAAGAAAAAGGCCCCGCGTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTagaa < 1:272795/143‑1 (MQ=255) aacaaTGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCg < 2:416670/140‑1 (MQ=255) aaaaaTGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAGCGGTGTAGAAGATCg < 2:5888/143‑1 (MQ=255) aaaTGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGt < 1:73494/143‑1 (MQ=255) gaTGCCCTGCGTAAGCGGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAACAGCTGCAGCGGAACGGTGTAGAAGATAgggg > 2:66243/2‑141 (MQ=255) aTGCCCTGCGTAAGCGGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCACCTGCAGCGGAACGGTGGAGAAGCTCgtcgc > 2:768695/1‑139 (MQ=255) aTGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTGGGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGc < 2:656306/143‑1 (MQ=255) cGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGAGCAATCAc > 2:711805/1‑143 (MQ=255) | TCAATTACAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCAC > NC_000913/3911677‑3911958 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |