Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,048,429 C→T 8.2% W88* (TGG→TAG) ‡ gfcC ← capsule biosynthesis GfcC family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,048,4290CT 8.2% 125.6 / 2.6 51W88* (TGG→TAG) ‡gfcCcapsule biosynthesis GfcC family protein
Reads supporting (aligned to +/- strand):  ref base C (16/30);  new base T (2/2);  total (18/33)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01

AACGGGGGATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTT  >  NC_000913/1048288‑1048564
                                                                                                                                             |                                                                                                                                       
accGGGGGATTGCTGTTCTCTTCCACTCGTACGAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCa                                                                                                                                        <  2:308104/141‑1 (MQ=255)
 aCGGGGGATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAg                                                                                                                                       <  1:552745/143‑1 (MQ=255)
 aCGGGGGATTGCTGCTCTCACCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAg                                                                                                                                       <  1:158199/143‑1 (MQ=255)
        aTTGCTGTGCCCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGaagcaa                                                                                                                                <  2:21460/143‑1 (MQ=255)
           gCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCTGGGaagcagaag                                                                                                                             <  1:723760/143‑5 (MQ=255)
           gCTGTGCGCCTCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCTGGGaagcagaag                                                                                                                             <  1:723625/143‑5 (MQ=255)
              gTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGCGATTGAGCAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCCAGCtgtg                                                                                                                          >  1:876311/1‑143 (MQ=255)
                tctcATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAATTGTTGTCGCATGGCTTTAAGCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGcc                                                                                                                        <  2:194967/143‑1 (MQ=255)
                 ctcATCCACTCGTACAAAGTCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCa                                                                                                                       <  2:343750/143‑1 (MQ=255)
                  tcagtcACTCGTAGTAAATCAGGGTCCAGTGTGACCGGTGAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCAt                                                                                                                      <  2:273683/138‑1 (MQ=255)
                  tcATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCAt                                                                                                                      >  1:490701/1‑143 (MQ=255)
                  tcATCCACTCGTACAAAATAAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGCGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCAt                                                                                                                      >  1:63767/1‑143 (MQ=255)
                        aCTCGTACAAAATCAGGGTCCAGTTTGACCAGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGtgccatgccaag                                                                                                                >  2:865223/1‑141 (MQ=255)
                           cGTACAAAATCCGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTg                                                                                                             <  1:567962/143‑1 (MQ=255)
                                  actCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCt                                                                                                      <  1:153447/141‑1 (MQ=255)
                                  aagcAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCt                                                                                                      <  1:146480/140‑1 (MQ=255)
                                   aTCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCtt                                                                                                     <  1:510742/143‑1 (MQ=255)
                                        gggcccgTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGGACGGATTTAATCTTGGCCGCCACGTCGTCGTCAGTTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAgc                                                                                                <  2:849312/137‑1 (MQ=255)
                                         gTCCAGTCTGACCCGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAgcg                                                                                               <  1:311376/143‑1 (MQ=255)
                                          tCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCTTGGGAGCAAGCCCTGCCATGACATGTTGGTAGTCTTTAAgcgc                                                                                              >  2:189808/1‑143 (MQ=255)
                                           ccAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCt                                                                                             <  1:262612/143‑1 (MQ=255)
                                            cAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCtt                                                                                            <  1:103516/143‑1 (MQ=255)
                                               tttGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTCAGCGCTTGAg                                                                                         >  2:349697/1‑143 (MQ=255)
                                                       gTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCACGAAGCAAGCTGTGCCATGCCATGTTGGCAGTCTTTAAGCGCTGTAGCTGTcgcc                                                                                 >  1:733818/1‑143 (MQ=255)
                                                            cGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCaga                                                                            <  1:585421/143‑1 (MQ=255)
                                                                  ggtatatTGGGATTGAGGAACTGCTGGCGCCCGGGTTTAATGGTGGCCGCCACATCTTCGTCAGCTTCTGCTACCCGGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTGAAGCGCTTTAGCTTTCGCCGCAGAGTCGGt                                                                      <  2:223117/140‑1 (MQ=255)
                                                                   tGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTc                                                                     <  2:769047/143‑1 (MQ=255)
                                                                     atatTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCGACATCGTCGTCCGCTACTGCGTTCCGTGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTGCCGGCAGCGTCGGCCAg                                                                   >  2:692197/1‑143 (MQ=255)
                                                                      tatGGAGATTGAGTAACGGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCGGCAGAGTCGGTCAGc                                                                  <  2:773470/143‑1 (MQ=255)
                                                                         tgagattgagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGGCTTTAAGCGCTTTAGCTTTCGCCGCAGGGGCGGTCAGCagg                                                               >  1:148631/1‑141 (MQ=255)
                                                                              ttgagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGAAATGTTGGTAGGCTTTAAGCGCTTTCGCTGTCGCCGCGGGGGCGGTGAGCAAGGCAcg                                                          >  1:370713/1‑142 (MQ=255)
                                                                               tgagTAACTGCTGGCGCACGGATGTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTGTGCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCa                                                         <  2:896906/143‑1 (MQ=255)
                                                                                gagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAg                                                        >  1:885439/1‑143 (MQ=255)
                                                                                gagTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCACCGCAGAGACGGTCAACAAGGCACCAg                                                        >  2:112287/1‑143 (MQ=255)
                                                                                      ctgctgGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGccacc                                                  <  1:913469/143‑1 (MQ=255)
                                                                                                   gcttgaaTCGTCGCCGCCACAGCGTCGTCAGTTTCTGCTTCCCAGGGAGCAAGCTGTGCCATGACATGGTGGTAGTGTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATcgcg                                     <  1:515383/138‑1 (MQ=255)
                                                                                                      ttAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTCCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAg                                  >  2:103093/1‑143 (MQ=255)
                                                                                                           cGTTGCGGCCACACCGTCGTCAGCTTCGGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCg                             <  2:57208/143‑1 (MQ=255)
                                                                                                            gTTGCCGCCAGATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTGTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCgg                            <  2:181533/143‑1 (MQ=255)
                                                                                                            gTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTGAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCACCAAGGCACCAGGCCACCAGAGGCGATCACGCAgcagcag                            >  1:498676/1‑137 (MQ=255)
                                                                                                              tGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGtt                          <  2:523127/143‑1 (MQ=255)
                                                                                                                    cacaTCGTCGGCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCa                    <  1:446492/143‑1 (MQ=255)
                                                                                                                           tcgtcgGCCTCTGCTGCCCACGAAGCAAGCTGTGCCATGACGTGTTGGTAGCCTTTAAGCGCTTTAGCTTGCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGGGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTg             <  2:474896/143‑1 (MQ=255)
                                                                                                                             gtcAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCCTGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGa           <  1:865223/143‑1 (MQ=255)
                                                                                                                                aGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTGACCAACTGGacaa        >  2:118809/1‑143 (MQ=255)
                                                                                                                                 gCTTCTGCTTCCCAGGAAGCAAGCTGTGTCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCCGAGGCGGTCAGCAAGGCAACAGGCCTCCAGCGGCGATCGCGCGGTTGCGGGTGCGTCACCAACCGGacaac       >  1:716232/1‑143 (MQ=255)
                                                                                                                                 gCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTGGTGTCCCCACCTGGacaac       >  1:195150/1‑143 (MQ=255)
                                                                                                                                  cTTCTGTTTCCCAGGTAGCAAGCTGTGTCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGacaaca      <  2:510780/143‑1 (MQ=255)
                                                                                                                                  cTTCTGCTTCCCCTCATGCAAGCTGTGCCACGACATGTGGTTAGTCTTTACGCGCTTTAGCGTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCCCCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGacaaca      <  2:636345/143‑1 (MQ=255)
                                                                                                                                   gcttGCTTCCTGGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACAt     >  2:73120/4‑143 (MQ=255)
                                                                                                                                      tccgtCCGAGGAAGCAAGCCGTGCCATGGCAGGCTGGTAGGCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCGGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACAtttt  <  2:615686/139‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                       
AACGGGGGATTGCTGTTCTCATCCACTCGTACAAAATCAGGGTCCAGTTTGACCGGTAAGCGACCAGTGATATTGAGATTGAGTAACTGCTGGCGCACGGATTTAATCGTTGCCGCCACATCGTCGTCAGCTTCTGCTTCCCAGGAAGCAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTT  >  NC_000913/1048288‑1048564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑