| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,560,455:1 | +G | 100% | pseudogene (151/758 nt) | glpR ← | DNA‑binding transcriptional repressor GlpR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 94.9% | 112.1 / ‑0.2 | 39 | pseudogene (151/758 nt) | glpR | DNA‑binding transcriptional repressor GlpR |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (19/18); total (19/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTG > NC_000913/3560323‑3560587 | cgcggcgAGCCTTGGGGGGTTTGCGCCGACTCGCTGCGGGCGAGGCGCTCTCTGTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAg < 2:614577/142‑1 (MQ=255) tCGAGCCATTGGCGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGttt < 1:129401/143‑1 (MQ=255) ccATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTc > 1:548677/1‑143 (MQ=255) ggtttGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCCGGCTGGCCTTGCGATCGTGCCACCGCGTCTTAACCGAACTGGAACGCTGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCt < 1:53948/141‑1 (MQ=255) aTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTGGGGATCGTGCCTGGGCGAGTTGACCGCGCGGGACTGCAGCGCCGCACCGCCAT‑GAT‑GGCGCGGGATCGTGCTTTGCTCCGCCAGccc > 2:151659/1‑141 (MQ=255) ttGCTCCGCCACGTGGCGGGCGATGCGCTCGTTTTCTTCGGTCTGGGTGGGCTGGCGAGCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAt < 1:1355/143‑1 (MQ=255) cTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGa > 1:325481/1‑143 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGGGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTGATGGaa > 1:362501/1‑142 (MQ=255) cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGt > 1:206827/1‑143 (MQ=255) gCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATGAGGTTTTGCTCCGCCAGCTCATTGAGGTc > 1:166666/1‑143 (MQ=255) aCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCGGGTTTTGCTCCGCGCGCTCACGGAGGTcgcg > 1:503633/1‑143 (MQ=255) cTTTGCCGGCCATGCGCTCCTTTTCTTCCGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCACGTTTTGCCCCGCCAGCTCATTGAGGTCgcgg < 2:408277/143‑1 (MQ=255) ttGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggcg > 1:533556/1‑143 (MQ=255) tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGa < 1:463365/143‑1 (MQ=255) gggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa < 2:183428/143‑1 (MQ=255) ggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGAGCAGGTTGTGCTCCGCCAGCTCATTGAGGTCGCGGCGAGTAg > 2:523352/1‑143 (MQ=255) cgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAG‑GGCGCAGGATCAGGGTTTGCTCCGCCAGCGCATTGAGGGCGCGGCGAAGAGGCTGCgg > 1:178039/1‑143 (MQ=255) gcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGGTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTATGAggg > 1:131819/1‑143 (MQ=255) ctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTg > 1:471796/1‑143 (MQ=255) tgggctgcGGGCTGGGTGGCCGTGCGGTCGTGCCGCGGCGTGGTAGCGGAAGTGGAAGGCAGCGGCGCACGGCCATGGGG‑GGG‑CAGGAGCAGGTTTTGCTCCGCCCGCTCATTGGGGTCGCGGCGAATAGTCTGCGGGCTGAc < 1:596659/136‑1 (MQ=255) tttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg < 1:476268/143‑1 (MQ=255) gcttcGGTCTGGGTGGCCTTGCGCTCGTGCCTCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGga < 2:417180/142‑1 (MQ=255) tcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa < 1:325258/143‑1 (MQ=255) cGGTCTGGGTGGCCTTGCGCTCGTGGCACAACGCGTTAACCGAACTGGAACGCGGCGCCGCACCGCCGT‑GGT‑GGCGGAGGAGCAGGGTTTGCGCCGCCAGCCCAGTCGGATCGGGGGGAATCGTCTGCCGGCCGACAgacgag > 2:249844/1‑139 (MQ=255) ggTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAt < 1:219735/143‑1 (MQ=255) cTGGGTGGCTTTTCGATGGTGCCACGGCGTGTTAACTGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCATGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct < 2:169075/143‑1 (MQ=255) ggggCCTTGCGGCCGTGTCACGGCGTGTTAACCGCGCTGGAAGGCAGCGCCGCACCGTCCT‑GATGGGG‑CTGGATCAGGTTTTGCTCCGCGAGCTCTTTGCGGACGCCGCTGTTGCTCTGCGGGCTGACTGAGAAATGCTCTAc < 2:445080/141‑1 (MQ=255) cGATGGGGCCCCGGCGTGGTGACCGGACTGGGACGCAGCGCGGCACCGCCAT‑GAT‑GGCGCACAATCAGGTTTTACTCCGTCGGTGTGTTGAGTTCGCGCCCAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc < 2:267752/143‑1 (MQ=255) aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg < 2:206827/143‑1 (MQ=255) ccACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAGACGCTCTGCCAGCTCTTCGGTACTGa > 2:65638/1‑143 (MQ=255) cACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGAc < 1:398966/143‑1 (MQ=255) aCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACa < 1:194820/143‑1 (MQ=255) aCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACa > 2:128434/1‑143 (MQ=255) gAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCGGGATTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg > 1:345486/1‑143 (MQ=255) gAAGGCAGCGCCGCACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCTGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg < 2:325481/143‑1 (MQ=255) tgCAGCGCCGCGCCGCCAT‑GCT‑TGCGCCGTATCCGGTTTTGCGCCGCCAGCTCATTTAGGTCGCGGCGCACAGTCGGCTGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAGCCCTGCTGTTTAACCAGTTc < 2:662583/142‑1 (MQ=255) cgccgcACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGagaa > 2:186009/1‑143 (MQ=255) gcACCGCCAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAGTGACCTAACCCTGCTGTTTCACCCGTTCGATAATAcc > 2:425003/1‑143 (MQ=255) ccAT‑GAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgtg > 1:543380/1‑143 (MQ=255) | AGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GAT‑GGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTG > NC_000913/3560323‑3560587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |