| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,637,645:1 | +T | 100% | coding (4/1500 nt) | pitA → | metal phosphate:H(+) symporter PitA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,637,645 | 1 | . | T | 87.8% | 105.0 / 10.0 | 41 | coding (4/1500 nt) | pitA | metal phosphate:H(+) symporter PitA |
| Reads supporting (aligned to +/- strand): ref base . (4/1); new base T (15/21); total (19/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑TA‑CATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATC > NC_000913/3637509‑3637770 | tataAGATAATTATTTTTTGAGTGACATCCATCCAGGGGGCAAACCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑ttacat < 1:551161/143‑6 (MQ=255)tataAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAACTGGCGTAACGTCCTATG‑C‑ttacat > 2:285522/1‑138 (MQ=255) gATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CAtttgtt > 1:625765/1‑143 (MQ=255) aTAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAGGCCTATATTCCACTTTGCCCGCGCCGCGAAAGTCCCGGATAATGGGCCGCGTCCATGCCCCCAAAAGGGCGAACGGCCCAAGG‑C‑TACCAtttgttt > 1:311408/1‑143 (MQ=255) tAATTATTTTTTGGGTTGAATCCATTCCGGGGGGCAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CAtttgtttg < 2:311408/143‑1 (MQ=255) aaTTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGc < 1:138228/143‑1 (MQ=255) aaTTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGc < 1:153764/143‑1 (MQ=255) ccacttcttGAGCGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTg < 1:512477/136‑1 (MQ=255) tattTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCTCTGGTAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAACTGGCGTAACGTCCTACGCC‑TA‑CCTTTGGTTGCggg > 2:324509/1‑143 (MQ=255) ttttGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATAGTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCC‑TA‑CATTTGTTTGCGGGCCTg > 1:67482/1‑143 (MQ=255) tttGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTgg > 1:61677/1‑143 (MQ=255) aGTGAACTCCATACAGGACGCGAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGAttt < 2:380522/143‑1 (MQ=255) tGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGc < 2:284137/143‑1 (MQ=255) tGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATATCCTCAAAATGGCGTACCGTCCTATG‑CTTA‑CATTCGTTTGCTGGACTGGATTTGc > 1:45260/1‑143 (MQ=255) tCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATAcc < 1:508856/143‑1 (MQ=255) ccATACAGGGGGCAAATCAAAAAAAGTCTATGTTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGACGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATACCg < 1:336620/143‑1 (MQ=255) ccATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGCTTGCTGCCCTGGACTCGCTTACCg > 2:96088/1‑143 (MQ=255) cATACAGGGGGAAAATCAAAAAAATTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATACCgg < 1:133901/143‑1 (MQ=255) aCAGGCGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATACCGGGCt < 2:522788/143‑1 (MQ=255) gggCAAATCAAAAAAAGTCTATGTTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCGTGTCCTCAAAAGGGCGTAGCCTCCTATG‑CTTA‑CATTTGTTTCCTGGCCTGGATTTGCCAACCGGGCTGttct > 2:38570/1‑141 (MQ=255) ggCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGtgatt > 2:433890/1‑143 (MQ=255) cAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAGAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgctt < 2:263770/143‑1 (MQ=255) aaaGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATCATGCGCAGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATGACGGGCTGGTATTATTGCGTGCCCt > 1:645576/1‑143 (MQ=255) caGTCTATGTTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAGTGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCTTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTg < 1:20029/142‑1 (MQ=255) gTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGGTATTATTGCTTGCAATGGc > 2:425694/1‑143 (MQ=255) tCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCt > 2:478989/1‑143 (MQ=255) tttCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtgtg < 1:479292/143‑1 (MQ=255) tttCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtgtg > 1:589805/1‑143 (MQ=255) tCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGACACGTCCTATGCC‑TA‑CACTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCt > 2:623462/1‑143 (MQ=255) ccgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGa > 1:368293/1‑143 (MQ=255) gcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAg < 2:625765/143‑1 (MQ=255) ccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATCGCGGGCTGTGAGTCTTGCTTGCACTGGCTTTTGTGCTGTTCTCCGAAGCCa > 2:504567/1‑143 (MQ=255) ccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTCCGAAGccc > 1:321945/1‑142 (MQ=255) gAAAGTCACTGATAATGCGCCGCGTTAATGTCCTCAAAATGGCGTAACGTACTATG‑CTTA‑CATTTGTTCGCTGGCCTGGATTTGCATACCGGGCAGTTATTATTGCTTGCACTGTCCTTTGTGCTGTTTTACGAAGCCATCaa > 2:242949/1‑143 (MQ=255) tcccTGATAATGGGCCGCGTTCGTGTCCTCAACATGGCGTAACGTCCTATGCC‑TA‑CATTTGTTGGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGtt < 1:68876/140‑1 (MQ=255) aCTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTc < 2:635845/143‑1 (MQ=255) cTGATAATTCGCCGCGTTCATGTCCTCAGAATGGCGGAACGGCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCAGACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTcc < 2:106151/143‑1 (MQ=255) tAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTCCTGGCCTGGATTTGCCTACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACTAAGCCATCAATGGTTTCCATGa > 2:63533/1‑143 (MQ=255) ccgcGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGcc < 2:61677/143‑1 (MQ=255) aTGTCCTCAAAAGGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGt < 1:365681/143‑1 (MQ=255) aaaaTGGCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCg < 1:509353/143‑1 (MQ=255) ggCGTAACGTCCTATG‑CTTA‑CATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATc < 1:261965/143‑1 (MQ=255) ggCGCACCGTCCTACG‑CTTC‑CATTTCTTTGCTGGCCTGGATTTGCATACCGGGCTGGTATTATTGCTTGCACTGGCGTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATc < 2:526133/143‑1 (MQ=255) | TATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑TA‑CATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATC > NC_000913/3637509‑3637770 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |