| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,661,969 | C→G | 100% | V55L (GTT→CTT) | iscR ← | DNA‑binding transcriptional dual regulator IscR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,661,969 | 0 | C | G | 98.7% | 167.8 / ‑5.9 | 60 | V55L (GTT→CTT) | iscR | DNA‑binding transcriptional dual regulator IscR |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (31/28); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCA > NC_000913/2661831‑2662105 | cgccCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGa < 2:591884/143‑1 (MQ=255) ccTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACATATGCGTCAACGGCGCTAATTACTTCGCCAACCGCGATGCTTCTGG‑CAACGTTGCATAACAGATAACCACGGCCTGCTCCACGTACGCTGCAAAGCAGACCa > 2:189793/1‑143 (MQ=255) tGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCCGGTCCACGTACGCTGGCACGCAGCCCAtg > 2:57225/1‑142 (MQ=255) ggCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTGCCCGTACGCTGGAAAGCCGACCAtta > 2:195666/1‑142 (MQ=255) gccgccTTTGCCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGGTGG‑CATCCTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTAc < 2:587476/143‑1 (MQ=255) ccgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCGTGTAAGCTGGAAAGCAGACCATTTTTACg > 1:86499/1‑143 (MQ=255) ccgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAACGCAGACCATTCTTAcc > 2:617639/1‑142 (MQ=255) gccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACATACGCTGGAAAGCAGACCATTTCTACGCa > 2:664324/1‑143 (MQ=255) tACCCTCACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACgg < 1:661627/143‑1 (MQ=255) cccTGACAACGGGTGGCATGTACAGATTCGTCAACGGCGCTAAGTAGTTCGCCAACGGCGATGCTGCATG‑CGTGTTTGCCTAGCAGATAACCAGAGCCTGGTCGCAGTACGGCGCAGAGGAGACCATTCTTACGCAGCAGGGa > 2:651548/1‑143 (MQ=255) cTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATGTTTACGCAGACGGGaaa > 1:246391/1‑143 (MQ=255) cAACGGGAGGCGTCTACAGATTCGTCAAGGGCGCTAATTGCTTCGCCAGCGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAg < 1:113008/143‑1 (MQ=255) cTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTGCAGCTaa > 1:197088/1‑143 (MQ=255) cTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATaa < 2:426925/143‑1 (MQ=255) cTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATaa < 1:548570/143‑1 (MQ=255) cTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATaa < 1:622756/143‑1 (MQ=255) aGATTCGTCAACGGCGCTAATTACTTAGCCAACGGCGATGCTGCTGG‑CATCTTTGCATAACAGATAACCACCGCATGGGCCACGAACGCTGGAAAGCAGACCATTTTTACGAAGGCGGGACAACAGGTGAGCCAGaacagaca > 2:372360/1‑136 (MQ=255) aGATTCGTCAACGGCGCTAAGTACTTCGCGAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGaaa < 2:496611/143‑1 (MQ=255) tctaaagggCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGCTAAGAAAGGGaa > 2:190961/8‑143 (MQ=255) cGTCAACGGCGCTAATTACTTCGCCAATGGTG‑TGGGGAGGGACGACGATGCCTAACAGATAACCACAGCCAGGGGCACGTACGCTGGAAAGCAGACCATATCACCGCAGACGGCAAAACAGACGTTCCACAACAGAAAAGGaa > 2:405784/1‑143 (MQ=255) gTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGaaa > 1:387202/1‑143 (MQ=255) gTCAACGGCGCTAATTACTTCGCCAACGGCGATACTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCGGGCAAAAGTCCAAAAGTAACCAGACCATTTTTACGCAGACTGGAAAGCAGTTGTTCCCGATACGAAAGGGcaa > 1:670615/1‑143 (MQ=255) tCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAAt > 2:427162/1‑143 (MQ=255) aaCGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGGTAAGAAAGGGGAATTc > 1:252693/1‑143 (MQ=255) aCGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAAATcc > 1:330594/1‑143 (MQ=255) cGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAACCAGTCGTTCCAGATAAGAAAGGGAAATTccc > 1:22263/1‑143 (MQ=255) ggCTCTAATTACTTCGCCACCGGTGATGCTGTTGG‑CATCTTTGCCGAACAGATAACCACCGCCTGGTCCACGTACGCTGGACCGCAGACCACTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCt < 1:135115/143‑1 (MQ=255) ggCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTGGTTCCAGATAAGAAAGGAACAGTCCCt > 2:129741/1‑143 (MQ=255) ttCGCCAACGGCGATGCTGCTGG‑CATCTTGGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa < 2:112990/143‑1 (MQ=255) tCGCCAACGGCGACGCTGCTGG‑CAGCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAat < 1:617639/143‑1 (MQ=255) cGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAata < 2:339093/143‑1 (MQ=255) cAACGGCGATGCTGCTGG‑CATCTTTGCCTCACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCa < 2:472130/143‑1 (MQ=255) cAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCa < 1:500659/143‑1 (MQ=255) ggCGATGCTGGTGG‑CATCTTTGCCTAACAGATAACCACGGCCTGGTCCAGGTACACTGGAAAGCGGACCATTTTGACGCAGACGGGAAAACAGTTGATCCAGATAAGAATAGGAAAGTGCCTGACGATTGGAAATATGAGACa > 2:344518/1‑143 (MQ=255) gATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTGCGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAACCCGTTGTTCCCGCTACGCACGGGCCATTCCCTTACGTTCGGAAATATCAGCCAACg > 1:161646/1‑143 (MQ=255) gATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACg < 2:463347/143‑1 (MQ=255) ctgG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTCCGCTGGAAAGCAGACCATCTTTACGCCGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGACATTCCCTGACGTCGGCAACTCTCAGCCAACGACACCgc > 2:308956/1‑142 (MQ=255) g‑CATCTTTGCCTATCATATATCTACCGTCTGTTCCACGTATGATGTAAAGCAGACCATATTTACGCAGACGGGAAAACAGTTGTTCCAGGGAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGcc < 1:282007/143‑1 (MQ=255) gcagcgttGCATAACAGATAACCACCGCCTGGGCCAAGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGcc < 1:193078/137‑1 (MQ=255) aTCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGCACGCAGACCATTTTTACGCAGACGGGCAAACAGTTGTTCCCGACAAGAAAGGGAAATTCCCAGACGCTCGGAACCAGCGGCCAACGGTACCGGGCCCg > 2:39540/1‑143 (MQ=255) tCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGTAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGc < 1:225246/143‑1 (MQ=255) tAACAGATAACCCCCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGt < 1:350968/143‑1 (MQ=255) aaCAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGtt < 1:551487/143‑1 (MQ=255) aaCAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGtt > 2:360127/1‑143 (MQ=255) cAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTGAGAGGTgg > 1:279407/1‑142 (MQ=255) aGATAACCACCGCCTGGGCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAg < 1:269990/143‑1 (MQ=255) gagaaCCACCGCCGGGTCCTCGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTGCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgc < 2:252693/140‑1 (MQ=255) ctcggcgtGGTCCAGGGACGGTGGGAAGCGGACCGTTTTTCCGCAGACGGGAGAGCAGTTGTTCCAGGTAAGAAAGGGAAATTGCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACg < 2:670615/136‑1 (MQ=255) caccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGACATATCAGCCAACGGTACCGAGCCCGCTTCAGAGCTGAGCGCAACg > 2:630828/1‑143 (MQ=255) caccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACg > 2:460204/1‑143 (MQ=255) caccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACg > 1:275599/1‑143 (MQ=255) accGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGt > 1:207982/1‑143 (MQ=255) cGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCa < 1:427162/143‑1 (MQ=255) gCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTCCGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCaa < 1:664324/143‑1 (MQ=255) gTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGCCGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCCTCAGAGTTGAGCGCAACGTCAAGCAtt > 2:660100/1‑143 (MQ=255) tCCACGGACGCTGGAACGCTACACATTTTTCAGCAGCCTGGAAAACAGTTGTTCCAGAGAAGGACGGGCAATTCCCTGACGCTCGGAAATATCAGCCAACGTTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTg < 2:579567/143‑1 (MQ=255) cACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCg < 1:76835/143‑1 (MQ=255) tACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCa > 2:380124/1‑143 (MQ=255) tGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGATCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCCTGGCGGTCACGGcg > 2:332681/1‑142 (MQ=255) aggAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCa < 1:512120/142‑1 (MQ=255) | CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGG‑CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCA > NC_000913/2661831‑2662105 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |