BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,041,632	T→A	100%	V107E (GTG→GAG)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,632	0	T	A	100.0%	92.9 / NA	36	V107E (GTG→GAG)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (10/26); total (10/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCT  >  NC_000913/3041502‑3041767

gACGCCAACGCTAACACGTGCAGCAATGTACGTCGGTCCCGCGCCGGCGATGGCTTCGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCGCACTGCACCGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGcc                                                                                                                             <  2:406576/143‑1 (MQ=255)

     cAAAGGTAAAGTGTGGAGCAATTTACGTCTGTGCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAgacg                                                                                                                        <  2:563898/143‑1 (MQ=255)

      aaaGGTAAAATGGGGAGCAATGTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAgacga                                                                                                                       <  2:130640/143‑1 (MQ=255)

         ggTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGAcgacga                                                                                                                    >  1:96053/1‑143 (MQ=255)

          gTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGacgacgcg                                                                                                                   >  2:68151/1‑141 (MQ=255)

               aTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCgtgt                                                                                                              >  2:668798/1‑143 (MQ=255)

                tgtgGAGCAATCTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGtgtg                                                                                                             <  1:77736/143‑1 (MQ=255)

                     aGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATGCTCTAAAGAGACCATCGCGCCCGACGACGAGCGTGCgcttct                                                                                                        >  2:38903/1‑143 (MQ=255)

                       cgatgtACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGGCTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                                                                                      <  2:221017/138‑1 (MQ=255)

                       aaaTTTACGTCCGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGTGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                                                                                      <  2:596647/142‑1 (MQ=255)

                        actgtACGTCTGTTCCGCGACGGGGATGGCTTTGAATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTgg                                                                                                     <  1:206444/139‑1 (MQ=255)

                          tttGCGGCTGTTCCGCGGCGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGAGGTATTCTCTAAAGAGACAATCGTGCCAGACGACGAGCGTGTGGTGGgtggtg                                                                                                   >  2:330764/1‑143 (MQ=255)

                              cGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACGGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGc                                                                                               <  2:68797/143‑1 (MQ=255)

                               gTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGcc                                                                                              <  1:62526/143‑1 (MQ=255)

                                tctatcccGCGCCGGCGATGGCTTTGCATCGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg                                                                                             <  2:541739/137‑1 (MQ=255)

                                     tCCGCGACGGCGATGGCTTTGTATGGATTGAACGGCGCAGCGTGCGTGAACCTCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGtttt                                                                                        <  1:81349/143‑1 (MQ=255)

                                        gcgACGGCGATGGCTTTGCATGGATTGAACGGCGTAGCGGGGGTGAACCGCAGCGGATGGAACTGAAAAAGTATGCGGGATTCTCTAAAGAGGCGATCGCGCGAGACGGCGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAg                                                                                     <  2:466912/143‑1 (MQ=255)

                                           aCGGCGATGGTTTTGCATGGATTGAACGGCGCAGCGTGCGTGAGCCGCAGCTGACTGAACTGACAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcg                                                                                  <  2:295255/143‑1 (MQ=255)

                                                     cTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgcgc                                                                        <  1:416102/143‑1 (MQ=255)

                                                          cATGCATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCa                                                                   <  1:316410/143‑1 (MQ=255)

                                                               aTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct                                                              >  1:294800/1‑143 (MQ=255)

                                                                   aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTACAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAGTCTCttt                                                          >  1:671507/1‑143 (MQ=255)

                                                                   aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCttt                                                          >  2:127687/1‑143 (MQ=255)

                                                                                     aaCCGAAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa                                        <  2:527234/143‑1 (MQ=255)

                                                                                      aCCGCAGCTGACTGAGCTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaag                                       <  1:101971/143‑1 (MQ=255)

                                                                                             cggactgaGCGGAAAAAATATGGTGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACa                                <  2:90332/141‑1 (MQ=255)

                                                                                              ggactgaACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAg                               <  1:196425/142‑1 (MQ=255)

                                                                                                  tgaactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAg                           >  1:643139/1‑143 (MQ=255)

                                                                                                  cggactgaaAAAATATGCGGGAGTCCGTAAAGAGGCCATCGCGCCGGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAg                           <  1:584307/140‑1 (MQ=255)

                                                                                                    aactgaaAAAATATGCGGTATGCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTc                         <  1:192180/143‑1 (MQ=255)

                                                                                                           aaaaCAGGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCAGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaag                  <  2:254975/143‑1 (MQ=255)

                                                                                                             aaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgc                <  2:45582/143‑1 (MQ=255)

                                                                                                                         tggctAAAGAGACCAGCGCGCCAGACGCGGGGCGGGTGCTGCGGGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTg    <  2:649271/140‑1 (MQ=255)

                                                                                                                         cctgtAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTCTGGCCGGTTTTCAGGCGCGCGCCCCACCAGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTg    <  2:588292/139‑1 (MQ=255)

                                                                                                                           tctAAAGAGACCATCGCGCCATATGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCt  <  1:330764/143‑1 (MQ=255)

                                                                                                                           tctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGAGCGCGCAGCTCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGAGAGGACAAGAAGGAGCGACCACATAGCt  >  2:244466/1‑143 (MQ=255)

GACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCT  >  NC_000913/3041502‑3041767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`