Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,168,653 G→A 8.5% intergenic (‑109/+96) mqsR ← / ← ygiV mRNA interferase/toxin of the MqsR‑MqsA toxin‑antitoxin system/DNA‑binding transcriptional repressor YgiV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,168,6530GA 8.5% 118.3 / 3.1 47intergenic (‑109/+96)mqsR/ygiVmRNA interferase/toxin of the MqsR‑MqsA toxin‑antitoxin system/DNA‑binding transcriptional repressor YgiV
Reads supporting (aligned to +/- strand):  ref base G (21/22);  new base A (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

AAACGTGTATGTGGTGTGCGTTTTTCCATAACCCCCGCCTCCCTGTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGGAAGCTGGAGTCAGGCATCACGCCAACGGCACATAAACATCCGTTTCCAGTCGCTGCTCTGT  >  NC_000913/3168516‑3168793
                                                                                                                                         |                                                                                                                                            
aaaCGTGTATGTGGTGTGCGTTTTTCCATAACCCCCGCCTCCCTGTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTc                                                                                                                                         >  2:202395/1‑143 (MQ=255)
     tgtATTTTGTGTGCGTTTTTCCATAACCCCCGCCTCCCTGTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTaa                                                                                                                                    <  1:438915/143‑1 (MQ=255)
        aTGTGGTGTGCGTTTTTCCATAACCCCCGCCTCCCTGTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGATTAGGTAGATGTACGGAGATAGTGCCCACACAAAACGTATTCTTTAAggc                                                                                                                                 >  2:7324/1‑142 (MQ=255)
         tgtgGTGTGCGTTTTACCATAACCCCCGCCTCCCTGTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGAAGATAGTGACCACACAAAACGTATTCTTTAAggca                                                                                                                                >  2:568059/1‑141 (MQ=255)
          gtggtgTGCGTTTTTCCATAACCCCCGCCTCCCTGTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGaaa                                                                                                                               <  1:357550/143‑1 (MQ=255)
                  cGTTTTTCCATAACCCCCGCCTCCCTGTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGa                                                                                                                       <  1:288197/143‑1 (MQ=255)
                   gTTTTTCCATAACCCCCGCCTCCCTGTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACATATTCTTTAAGGAAAGTGATTGAc                                                                                                                      >  1:478342/1‑143 (MQ=255)
                   gTTTTTCCATAACCCCCGCCTCCCTGTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACATATTCTTTAAGGAAAGTGATTGAc                                                                                                                      >  1:478275/1‑143 (MQ=255)
                             aaCCCCCGCCTCACTACTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGaa                                                                                                            <  2:50758/143‑1 (MQ=255)
                              aCCGCCGCCTCCCTGTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAATGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGaaa                                                                                                           <  1:215764/143‑1 (MQ=255)
                              aCCCCCGCCTCCCTGTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGaaa                                                                                                           <  1:1079/143‑1 (MQ=255)
                                  cccacTCCCTGTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATGCTTTAAGGAAAGTGATTGACCATATAAGAAAGTgg                                                                                                       <  2:273859/139‑1 (MQ=255)
                                      cttccTGTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCa                                                                                                   <  2:530868/140‑1 (MQ=255)
                                       tCCCTGTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTGAGTTAGATGTGCGGAGATAGTGACCACACAAAACGGATTCTTTAAGGAAAGTGCTGGACCATTGAAGAAAGTGGCGCaa                                                                                                  >  2:363185/1‑142 (MQ=255)
                                        cccTGTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCAtt                                                                                                 >  1:664419/1‑143 (MQ=255)
                                         ccTGTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATta                                                                                                <  1:222924/143‑1 (MQ=255)
                                            gTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAAACAGGAGGACAAATGTTGGCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATtagta                                                                                             >  1:669769/1‑143 (MQ=255)
                                              ttagaaaGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAgc                                                                                           <  2:394814/137‑1 (MQ=255)
                                                cTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAgcgc                                                                                         >  1:531352/1‑143 (MQ=255)
                                                cTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAgcgc                                                                                         >  1:60999/1‑143 (MQ=255)
                                                      ttATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGGGCCAGtct                                                                                   >  2:53114/1‑141 (MQ=255)
                                                       tataACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGGTTg                                                                                  >  2:157952/1‑143 (MQ=255)
                                                         taACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACATATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGaa                                                                                <  2:478275/143‑1 (MQ=255)
                                                         taACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACATATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGaa                                                                                <  2:478342/143‑1 (MQ=255)
                                                               aaaaGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGa                                                                          <  1:524756/143‑1 (MQ=255)
                                                                 aaGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCAGTAGTAGCGCAGGTTTGAAGAAGGCAt                                                                        >  1:262499/1‑143 (MQ=255)
                                                                  aGGTTAATTACAGCAATGAAAAAGCACCTAACAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATGGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTCACGCAGGAAtt                                                                       >  2:428582/1‑143 (MQ=255)
                                                                  aGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAAtt                                                                       >  1:39177/1‑143 (MQ=255)
                                                                   ggTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAAgtt                                                                      >  2:341186/1‑143 (MQ=255)
                                                                                aaTGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGGAAGcag                                                         >  2:490883/1‑141 (MQ=255)
                                                                                    aaaaaGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGGAAGCTGGAGt                                                     >  1:199517/1‑143 (MQ=255)
                                                                                      aaaGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGGAAGCTGGAGTCa                                                   <  1:172550/143‑1 (MQ=255)
                                                                                             cTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGGAAGCTGGAGTCAGGCATCa                                            >  1:646269/1‑143 (MQ=255)
                                                                                                aaaGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGGAAGCTGGAGTCAGGCATCACGc                                         <  2:78945/143‑1 (MQ=255)
                                                                                                  aGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTGTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGGAAGCTGGAGTCAGGCATCACGCCa                                       <  1:412389/143‑1 (MQ=255)
                                                                                                         ttagATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGGAAGCTGGAGTCAGGCATCACGCCAACGGcac                                >  1:295654/1‑143 (MQ=255)
                                                                                                         ttagATGTACGGAGATAGTGACCACACAAAACGTATACTTTAAGGAAAGTAATTGACCATATAAGAAAGTGGCGAATTAGTAGCGCCAGTACGGAACAGGAAGTAATAAGGGAAGCTGGAGACAGGCATCACGCGAACGGcac                                >  2:578388/1‑143 (MQ=255)
                                                                                                            gATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGGAAGCTGGAGTCAGGCATCACGCCAACGGCACATa                             <  1:545031/143‑1 (MQ=255)
                                                                                                                tACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGGAAGCTGGAGTCAGGCATCACGCCAACGGCACATAAACa                         >  2:508781/1‑143 (MQ=255)
                                                                                                                   ggAGATAGTGACCAGTACAAATGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGGAAGCTGGAGTCAGGCATCACGCCAACGGCACATAAACATcc                      <  2:316219/143‑1 (MQ=255)
                                                                                                                        tAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGGAAGCTGGAGTCAGGCATCACGCCAACGGCACATAAACATCCGTTTc                 <  1:236903/143‑1 (MQ=255)
                                                                                                                             aCCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGGGAGCTGGAGTCAGGCATCACGCCAACGGCACATAAACATCCGTTTCCAGTc            <  1:51490/143‑1 (MQ=255)
                                                                                                                               cacacaAACCGTATTCTTTAAGGAAAGTGATTGACCATATAACAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGGAAGCTCGAGTCAGGCATCACGCCAACGGCACATAAACATCCGTTTCCAGTCgc          <  1:467708/143‑1 (MQ=255)
                                                                                                                                    aaaaCGTACTCTTTAAGGACAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTCTGAAGCAGGAATTTATAAGGGACGCTGGAGTCAGGCATCACGCCAACGGCACATAAACATCCGTTTCCAGTCGCTGctc     <  1:326389/143‑1 (MQ=255)
                                                                                                                                     aaaCGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGGAAGCTGGAGTCAGGCATCACGCCAACGGCACATAAACATCCGTTTCCAGTCGCTGctct    <  1:429185/143‑1 (MQ=255)
                                                                                                                                       ccGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGTAAGCTGGAGTCAGGCATCACGCCAACGGCACATAAACATCCGTTTCCAGTCGCTGCTCTGt  <  1:159208/142‑1 (MQ=255)
                                                                                                                                       aCGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGGAAGCTGGAGTCAGGCATCACGCCAACGGCACATAAACATCCGTTTCCACTCGCTGCCCTGt  >  1:55546/1‑143 (MQ=255)
                                                                                                                                         |                                                                                                                                            
AAACGTGTATGTGGTGTGCGTTTTTCCATAACCCCCGCCTCCCTGTTACTTTAGTTATAACCTAAAAGGTTAATTACAGCAATGAAAAAGCACCTAAAAGGTTAGTTAGATGTACGGAGATAGTGACCACACAAAACGTATTCTTTAAGGAAAGTGATTGACCATATAAGAAAGTGGCGCATTAGTAGCGCCAGTTTGAAGCAGGAATTTATAAGGGAAGCTGGAGTCAGGCATCACGCCAACGGCACATAAACATCCGTTTCCAGTCGCTGCTCTGT  >  NC_000913/3168516‑3168793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑