Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5430CA100.0% 154.2 / ‑6.7 51P1100Q (CCG→CAG) rpoBRNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (28/22);  minor base G (0/1);  total (28/23)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGC  >  NC_000913/4184404‑4184683
                                                                                                                                           |                                                                                                                                            
cTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCt                                                                                                                                           <  1:284699/143‑1 (MQ=255)
 tGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGCTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTg                                                                                                                                          >  1:315698/1‑143 (MQ=255)
 tGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTg                                                                                                                                          >  1:34400/1‑143 (MQ=255)
  ggCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                         <  1:342694/143‑1 (MQ=255)
              ccGTAGCCAGCCGGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc                                                                                                                             <  1:296424/143‑1 (MQ=255)
                  aTCCAGCCTGGTGACGAGATTGCAGGTTGTGATGGTAACTAGTGTGTTATTTCTTAGATCATCCCGATCGATGATATGCCTTACGATGAAAACGGTACGCCGGTATACATCGTACTGAACCAGCTTGGCGTACCGTCTCGTAt                                                                                                                         <  2:440166/143‑1 (MQ=255)
                  aTCCAGCCTGGTGACAAAATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACAATGAAAACGGTACGCCGGTAGACAACGTACACAACCAGTTGGGCGAACCGCCCCGTac                                                                                                                         >  2:93874/1‑142 (MQ=255)
                     cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                      <  1:266299/143‑1 (MQ=255)
                     cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCAACGAGCCCGCATACAGCAAAAGAAAAACCGCAGTAGACATCGTACTGAACCAGCGGGGCGTACCGTCGCGTATGaa                                                                                                                      >  2:656628/1‑143 (MQ=255)
                          tGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAAACCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                                 >  1:168035/1‑143 (MQ=255)
                             tGAAAAGATGGTTGGTCGTTACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCGTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc                                                                                                              <  1:8145/143‑1 (MQ=255)
                              gACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCACGTATGAACATCGGTcc                                                                                                             >  1:557781/1‑142 (MQ=255)
                                   ggtGGCCGGTCGTCACGCGAACAAGGGTGTAATGTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCATCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATcc                                                                                                        <  2:47287/141‑1 (MQ=255)
                                    ctGGCGGGTCGTCACGGTAACACGGGTGTAATTTCTAAGATCGACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt                                                                                                       <  2:605524/142‑1 (MQ=255)
                                     tGGCAGGTCGTCACGGTAACAAGGGTCTAATTTCTAAGATCACCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                      <  2:427082/143‑1 (MQ=255)
                                     tGGCAGGTCGTCACGGGAACAAGGGTGGAATTTCGAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                      <  2:427533/143‑1 (MQ=255)
                                          ggTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAATATATGCCTTACGATGAAAACGGTACGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAc                                                                                                 <  1:514485/143‑1 (MQ=255)
                                           gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAcc                                                                                                >  1:186094/1‑143 (MQ=255)
                                              gtcACGGTAACAAGGGTGTAATTTCTAAGATCAACACGATCGAAGATATGCCTTACGATGAAAAAGGTACGCCGGTAGACATCGTACTGAACCAGATGGGCGTACCGTCTCGTATGAACAGCGGTAAGATCATCCAAACCCAc                                                                                             >  2:353093/1‑143 (MQ=255)
                                               tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGCAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGCCCCGTCTCAACACCGGTCCGACCCGCGAAAcccccc                                                                                            >  2:68399/1‑143 (MQ=255)
                                                                tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                           >  1:494612/1‑143 (MQ=255)
                                                                tAATTGCTAAGAGCAACCCGATCGAAGATATGCATGACGATGAAATCGTTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                           <  2:199789/143‑1 (MQ=255)
                                                                      cTAAGATCGTCCCGATCGAAGATATGCCTTACGATGAACACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATc                                                                     <  2:68955/143‑1 (MQ=255)
                                                                           aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                                <  1:262449/143‑1 (MQ=255)
                                                                           aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTCACCCAGCGGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCCGCGa                                                                >  2:442331/1‑143 (MQ=255)
                                                                              aaCCCGATCGAAGATATGTCTTACGATGAGACCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                             <  2:315698/143‑1 (MQ=255)
                                                                                cccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGGATGGCTGCGAACGGTATCGACGACAAGa                                                           >  1:88194/1‑143 (MQ=255)
                                                                                cccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGCTATGGCTGCGAAAGGTATCGGCGACAAGa                                                           >  2:479291/1‑143 (MQ=255)
                                                                                cccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTAGGCGTACCTTCTCGTATGCACATCGGTCAGATCCTCGACACCCACCCGGGTATAGCTGCGCACGGTAGCGGGGACAAGa                                                           >  2:166854/1‑143 (MQ=255)
                                                                                 ccGAGCGGAGATATGCCTTCCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCGCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                          <  2:88910/143‑1 (MQ=255)
                                                                                  cGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGGCAAGATc                                                         >  1:133904/1‑143 (MQ=255)
                                                                                     tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTACCGGCGACAAGATCAAc                                                      >  2:605305/1‑143 (MQ=255)
                                                                                     tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCGCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTCTGGCTGCGGAAGGTGTCGGCGACAAGGTCAAc                                                      >  1:79438/1‑143 (MQ=255)
                                                                                                ccTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaa                                           >  1:583485/1‑143 (MQ=255)
                                                                                                  ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACACGATCGACGCCATGCTGAAAc                                         >  1:239900/1‑143 (MQ=255)
                                                                                                        tGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc                                   >  1:362893/1‑143 (MQ=255)
                                                                                                         gAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGACACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGCCAAGATCAACGCCATGCTGAAACAgcagca                                  >  1:176835/1‑143 (MQ=255)
                                                                                                             aCGGTACGCCGGTAGCCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                              <  1:354461/143‑1 (MQ=255)
                                                                                                                 tACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                          <  1:582091/143‑1 (MQ=255)
                                                                                                                 gaCGCCGGTAGACATCGTACTGAACAAGCTGGGGGTACCGTCGCGTATGAACAGCGGTGAGAGCCTCGAAACCCAGCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                          <  1:228023/142‑1 (MQ=255)
                                                                                                                  aCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc                         >  1:66972/1‑143 (MQ=255)
                                                                                                                   cGCCGGTAGACATCGTACGGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgcg                        <  1:320620/143‑1 (MQ=255)
                                                                                                                    gCCGTTAGACATCGTACTGAACCAGCTGTGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGa                       <  2:51250/143‑1 (MQ=255)
                                                                                                                      cGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAGCCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACACGATCAACGCCATGCTGATACAGCAGCAAGAAGTCGCGaac                     >  2:368740/1‑142 (MQ=255)
                                                                                                                      cGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGCACGGCTGCGACAGGTATCGGCGACAAGATCAACGCCATGCTGAAGCAGCAGCAAGAAGTCGCGaac                     >  2:363736/1‑142 (MQ=255)
                                                                                                                      cGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGaaa                     >  2:423187/1‑143 (MQ=255)
                                                                                                                             cATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGcgcg              >  1:626015/1‑143 (MQ=255)
                                                                                                                               tCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGGATCTCGGCACCGGCCTGGGTATGGCTGCGCTAGGTATTGGCGACAAGATCAACGCCATGCGGCAACAGCCGGAAGCGGGCGAGACACTgcgagcc            >  2:267678/1‑139 (MQ=255)
                                                                                                                               gcgcaCTGAACCGGCAGGGCCTCCCGTGTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGaa            <  2:437396/139‑1 (MQ=255)
                                                                                                                                 actcTGAACCAGCTCGGCGTACCGCCTCTTAGGAACAGCGGTAAGAGCCGCGAAAACGCCGTAGGGAGCGCTTCTAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAtt          <  2:164376/140‑1 (MQ=255)
                                                                                                                                  tACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAAGGCTGCGCAAGGTATCGGCGACACGATGAAAGCCAGGCAGGAACAGCAGCCAGAACGCGGGAACCTGCGAGaagac         >  2:99460/1‑140 (MQ=255)
                                                                                                                                         cccGCTGGGCGTACCGTCTCGTACGAACATCGGTCAGATCCTCGACACCCACCTGGGTATGGCTGCGAAAGGTAGCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAGTCATCCAgc  <  2:478594/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGC  >  NC_000913/4184404‑4184683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑