| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,196,196 | T→C | 32.6% | intergenic (‑97/+136) | thiC ← / ← rsd | phosphomethylpyrimidine synthase/regulator of sigma D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,196,196 | 0 | T | C | 32.6% | 35.8 / 22.2 | 37 | intergenic (‑97/+136) | thiC/rsd | phosphomethylpyrimidine synthase/regulator of sigma D |
| Reads supporting (aligned to +/- strand): ref base T (16/8); new base C (4/8); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.15e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
GCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCA > NC_000913/4196056‑4196327 | gCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTc > 1:431000/1‑143 (MQ=255)gCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTc < 2:126406/143‑1 (MQ=255) tgttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATAGCCGCAGGGGCGATGCGATAGCAGATTAACGCATGCTCCCTTCGAAggaa > 2:602907/1‑141 (MQ=255) gcggcgGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGc > 2:592121/1‑143 (MQ=255) cgctggTCAGTTTTCTTGCAGCCATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGCGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCt < 1:76873/139‑1 (MQ=255) gttgtgcAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTATGGATCTTGTTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCTGTTGCGTGGTGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTg < 2:108602/137‑1 (MQ=255) aCATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCCGGTATTAGCCTGATCAGGTTCCGCGGATCCCg > 2:96096/1‑143 (MQ=255) tAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAAt < 1:324242/143‑1 (MQ=255) tCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTCACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCGGAATaacc > 2:533710/1‑141 (MQ=255) cATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCACAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTAGCAGGTATTAGCGTGATCAGGTTCTGCGGATCCCGAAGAAACg > 2:131715/1‑143 (MQ=255) ttgcAAAAAGTTAAGGGCGTGGCTGGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGt < 2:634586/140‑1 (MQ=255) tgaaaGGTAAGGGCGTGGCGTGTCCGACGAGGGATGAAGCGAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATGAACTCTTGTTCCCTTCGCATGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAATCGGttcca < 2:448568/141‑5 (MQ=255) aaaaaGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTGTCa > 1:511918/1‑143 (MQ=255) aGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTATCAGCCa < 1:241168/143‑1 (MQ=255) tAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTATCAGCCAGtt > 1:40747/1‑143 (MQ=255) aGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTaa < 1:496498/143‑1 (MQ=255) ggtgtcggCTTGTCGGGCGACGGATGAATCAGGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACGCTGGCTCCCTTCGCAGGTATTAGCCTGATGAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAg < 2:669702/137‑1 (MQ=255) ggACCTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTATCAGCCAGTTAAg < 1:592314/143‑1 (MQ=255) gACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGAACCCCCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTATCAGCCAGTTAAgg > 1:25310/1‑143 (MQ=255) aCGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGAGGCGATGCGATAGCAGATTCACTCTTGTTCCCGTCGCAGGTATTAGACTGATCAGGTTCGGAGGGTCACGAAATAACGGAATCAGCCAGTTAAGGc > 2:53276/1‑143 (MQ=255) ggCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCGCTc > 1:86714/1‑143 (MQ=255) ttGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGa > 1:651728/1‑143 (MQ=255) gacgacGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGCCAAGaa > 2:606269/1‑143 (MQ=255) acgGAAGAATCAACTGACGAGTGCCGCAGGGGCGATGCGATAGCAGATGATCTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTATCGGCCAGTTAAGGCACTCCGACAAGAAATTa < 2:590757/143‑1 (MQ=255) gATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCGTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGcc < 1:202917/143‑1 (MQ=255) tGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCCGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGcccc < 2:40859/143‑1 (MQ=255) gAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATCAGCCTGAGCAGCTTCCGCGGGTCCCGACTAAACGGTCTCAGCCAGTTAAGGCACGCCGACAAGAAAAGAGCcgcg > 2:429577/1‑143 (MQ=255) cAAGCGACCATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCaaa < 1:375549/143‑1 (MQ=255) aaGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCCCAGCCAGTTAAGGCACTCCGACAAGAAAATAGCCCCGCAAAt > 2:513829/1‑143 (MQ=255) gagcCGATCGGCGCCGGCGCGATGCGATAGCAGATGAGCTCTTGCGCTCTTCGCAGGTATTAGCCTGATCACGTTCCGCGCATCCTGTATAAACGGTCGCAGCCAGTTAAGGCACTCAGACAAGAAATTAGCCCCGCAAATgg < 2:101414/143‑1 (MQ=255) agaCGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATggg > 1:133153/1‑143 (MQ=255) agaCGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATggg > 1:157944/1‑143 (MQ=255) aCGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGCTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGc > 1:288773/1‑143 (MQ=255) cccctgcggCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAAt > 1:206756/8‑143 (MQ=255) gcAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTATCAGCCAGTTAAGGCACTCCGCCAAGAAATTAGCGCCGCAAACGGGGCGTTGAATGt > 2:213593/1‑143 (MQ=255) caGCATATTAACTCTTGGTCCCTTCGCAGGTATTAGCCTGATCAGGGTCCGCGGATCGCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAAc < 2:321761/142‑1 (MQ=255) aTTAACTCCTGTTACCTTCGTAGGTATGAGCCTGATCAGGTTCCGCGGACCCCGAATAAACGGTCTCAGCCAGTTAAGGCACGCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCa < 2:599829/143‑1 (MQ=255) | GCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCA > NC_000913/4196056‑4196327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |