| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,341,824 | T→G | 33.8% | intergenic (‑196/‑87) | melR ← / → melA | DNA‑binding transcriptional dual regulator MelR/alpha‑galactosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,341,824 | 0 | T | G | 33.8% | 37.3 / 24.7 | 46 | intergenic (‑196/‑87) | melR/melA | DNA‑binding transcriptional dual regulator MelR/alpha‑galactosidase |
| Reads supporting (aligned to +/- strand): ref base T (13/16); new base G (8/7); total (22/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
TGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTC > NC_000913/4341684‑4341961 | tGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACCCAGGAACCGAGTGTa > 1:270293/1‑143 (MQ=255) gAGTGGGAGCACGGTTTTCACCCTCTTCCCAGGGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATgg < 2:100324/143‑1 (MQ=255) gAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGGAAACTCTGCTTTTCAGGTGATTTGTTCCCATAAATGCAGATTTACTGCTGCTTCACGCAGGATATGAGTTTATgg > 1:525527/1‑143 (MQ=255) gAGGGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATgg < 1:224960/143‑1 (MQ=255) tGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGaa < 2:199610/143‑1 (MQ=255) aaGCACGGTTTTCACCCTCTTCCCAGCGGGCAGAGGGAACTCACCGAGAATCATAAGGCCGAAAACTCTGCTTTTCAGGTAATTTATACCCAGAAACTCAGAGTTACTGCTGCTTCACACAGGCACGGAGTTTAAGAGAAtac > 2:171511/2‑141 (MQ=255) ggTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAAcc > 1:39155/1‑143 (MQ=255) ggtttCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTCATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCGGGATCTGAGTGTATGGGAATGCTCAAccc > 2:369922/3‑142 (MQ=255) tctTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAACACTCGGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGa > 1:528218/1‑143 (MQ=255) tctTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGa < 1:187528/143‑1 (MQ=255) cccAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACGCAAAATCAATTCTGCTTCACGCAGGATCTGAGTATATGGGAAGGCTCAACCTGGAAGCCGGCGGtt > 2:584439/1‑143 (MQ=255) gagGGGCGAGGGGACTCTTCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCt < 1:264258/143‑1 (MQ=255) ggCGAGGGGTCTTTCCGAGTATCATGAGGCCGAAGACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTAAGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAg < 1:29284/143‑1 (MQ=255) ggggCTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGc < 1:407758/143‑1 (MQ=255) ggACTCTGCGAGTATCATGAGGCCGAAAGCTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATCCTCAACCTGGAAGCCTGAGGTTTTCTGCAGATTCGcc < 2:111858/143‑1 (MQ=255) tctcCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGcc > 2:427951/1‑143 (MQ=255) tctcCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGGTTTCTGCAGATTCGACTgac > 2:348191/1‑141 (MQ=255) tctcCGAGTATCATGAGGCCGAAAACTCTGCTTTGCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGcc < 2:646056/143‑1 (MQ=255) tcCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGCGGTTTTCTGCAGATTCGCCTGCCat > 1:232606/1‑143 (MQ=255) gAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACACAGCTTTACTACTGCTTCATGCAGGATCTGAGCGTATGGGAATGCTCAACCTGGAAGCCGGAAGTTTTCTGCAGATTCGCCTGCCatgca > 2:266961/1‑141 (MQ=255) gAGTATCATGAGGCCCAAACCTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCACCCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCatgat < 1:481465/143‑1 (MQ=255) cATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGtt < 1:580657/143‑1 (MQ=255) gCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTcat > 2:670112/1‑142 (MQ=255) ccGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTcaag < 1:626879/143‑1 (MQ=255) tctGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAg < 2:672480/143‑1 (MQ=255) tctGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAg > 2:15313/1‑143 (MQ=255) gctGCTTTTCAGGTAAGTTGTGCCCTTGGTCTCAGGTTTACTGCTGCTTCACGCAGGATCTGAGGTTATGGGAATGCTCAACCTGGAGGCCGGAGGTTTTGTGGTGCTAACTGTTTCACGATGAAGTTAGTCAAGCAAGCCAg < 2:481745/142‑1 (MQ=255) tttCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATTCGCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGATATTCAAGCAAGCCAGGAGATc > 2:657954/1‑143 (MQ=255) ggTAATTTATGCCCATAAACTCAGATTTACTGCTGCTGCACGCAGGATCTTAGGTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCGTGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCat < 1:435292/143‑1 (MQ=255) gTAATTGATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCatg < 1:328786/143‑1 (MQ=255) tgtcttATTCCCCCGAACTCAGGTTTACTGCTGGTGCGGGGAGGGTCTGAGTTGATGGGAATGCTCAACCGGGACGTCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCatga < 1:522605/139‑1 (MQ=255) tAATTTATTCCCATAAACTCAGATTTACGGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCatga < 1:167857/143‑1 (MQ=255) aTTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGCACGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGCAGTTATTCAAGCAGCCCAGCAGAGCTCCatgatg > 2:665766/1‑143 (MQ=255) tctATCCCCATAAACTCAGATTTACTGCTCGTTCACGCAGGATCTCAGTCTATGGGAATGCTCAACCTGGAAGCCGTAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGt < 2:660110/141‑1 (MQ=255) tATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAACCAGGAGATCTGCATGATGTCt > 1:625258/1‑143 (MQ=255) cttCCTATATTCTCTGATTTACTGCTTCTTCATGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTGCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTg < 1:35312/142‑1 (MQ=255) tCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCGAGCCAGGAGATCTGGAAGATGTGTGCACCCACCATTa > 1:303235/1‑143 (MQ=255) tCAGATGTACTGCTGCTTCACGCAGGATCTTAGTTTATTGGAATGCTCAACCTGGAAGCCGGAGGTTTCCTGCATATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTa < 2:525527/143‑1 (MQ=255) tCAGATCTACTGCTGCGTCACGCATGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGACGCTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTa < 2:315401/143‑1 (MQ=255) gATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGACGTCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACAt > 1:125176/1‑143 (MQ=255) tgctgcTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCgg > 1:545350/1‑143 (MQ=255) tgctgcTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCCGAACCCAAAATTACATTTATCgg > 1:632878/1‑143 (MQ=255) tgctgcTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGCGGTTTTCTGCAGATTCGCCTGCCCTGATGAAGTTATTCAAGCAAGCCAGGCGATCTGCATGATGTCTGCACCCCAACTTACATTTATCgg > 1:114618/1‑143 (MQ=255) gCAGGATCTGAGTTTATGGGAATGCCCAACCTGGAAGCCGCAGGGTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTcga < 2:498929/143‑1 (MQ=255) gATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGAt > 2:217949/1‑143 (MQ=255) taggTTTAGGGGAATGCTCAACCTGGAAGCCGGAGGTGTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTc < 2:278153/140‑1 (MQ=255) | TGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTC > NC_000913/4341684‑4341961 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |