| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 397,574 | C→T | 18.9% | L312L (CTC→CTT) | sbmA → | peptide antibiotic/peptide nucleic acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 397,574 | 0 | C | T | 18.9% | 37.2 / 4.1 | 23 | L312L (CTC→CTT) | sbmA | peptide antibiotic/peptide nucleic acid transporter |
| Reads supporting (aligned to +/- strand): ref base C (12/6); new base T (2/2); total (14/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
GCCGGGGCTGGAGTTTAAAAACCAGCGTGTAGAGGCTGCCTACCGTAAAGAGCTGGTTTATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATAACGTTTTCGGTTTGTTCTTGCTGTTTCCGTCAATTGTTGCCGGTACGATTACGCTCGGCCTGATGACGCAGATTACCAACGTTTTTG > NC_000913/397433‑397716 | gCCGGGGCTGGAGTTTAAAAACCAGCGTGTAGAGGCTGCCTACCGTAAAGAGCTGGTTTATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAACTATTTCCGCctct < 1:516891/143‑1 (MQ=255)gCCGGGGCTGGAGTCTAAATGCCAGAGTGTAGCGGCTGCCCGCCGTAAAGGGTTGGTTTATGGTGAAGGCGATGCCCCGCGCGCGCCGCCGCCTACGGTACGCGCGCTGTTTAGCGCCGTACGGAAAAACTATTTCCGCctct < 1:474316/143‑1 (MQ=255) tttAAAAACCAGCGTGTAGAGGCTGCCTACCGTAAAGAGCTGGTTTATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAACACTCTTTCCGCCTCTATCTTCACTatat > 1:465961/1‑143 (MQ=255) tttAAAAACCAGCGTGTAGAGGCTGCCTACCGTAAAGAGCTGGTTTATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAACTATTTCCGCCTTTATTTTCACTatat > 2:494611/1‑143 (MQ=255) tttAAAAACCAGCGTGTAGAGGCTGCCTACCGTAAAGAGCTGGTTTATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAACTATTTCCGCCTTTATTTTCACTatat > 2:494604/1‑143 (MQ=255) aaCCAGCGTGTAGAGGCTGCCTACCGTAAAGAGCTGGTTTATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAACTATTTCCGCCTCTATTTTCACTATATGTAttt > 1:410189/1‑143 (MQ=255) cAGCGTGTAGACGCTGCCTCCCGTAAAGAGCTGGTTTATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCaa < 2:612530/143‑1 (MQ=255) gTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAACAACTATTTCCGCCTCTATTTTCAACATATGTATGGCAACATCGCCCGCCTTCCCTATTTGCAGGTCGATAACGTGTTc > 2:43670/1‑143 (MQ=255) gTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAACTATTTCCGCCTTTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATAACGTTTTc < 1:494604/143‑1 (MQ=255) gTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAACTATTTCCGCCTTTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATAACGTTTTc < 1:494611/143‑1 (MQ=255) ccgacgaCGCGCGCCACTCCCCCTACGGTATGCGTGCTGTTCACGGCCAGACGGAAGATCTATTTCGGATTGCTTTGTCTCTATGTGTATTTCAACATCGCCCGCATTTTCTATTTGCAGGTCGATAACGTTTTCGGTTTGtt < 2:588155/137‑1 (MQ=255) cgcgcgcgACGCCGCCTACGGTACGCGAGCTGTTTCGAGCCGTACGGGAACTCTATTTCGCCCTCTCTTTTCACCATATGTATTTCAACATCGCCCGCATTCTCTATTTGCCGGTCGATCAAGATTTCGCTTTGTTGTTGCTg > 2:124724/1‑143 (MQ=255) gcgcgACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATAACGTTTTCGGTTTGTTCTTGCTGttt < 2:598494/143‑1 (MQ=255) cgACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATAACGTTTTCGGTTTGTTCTTGCTGTTTCCg < 1:475950/143‑1 (MQ=255) gACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTCTTTGCAGGTCGATAACGTCTTCGGTTGGCTCTTGCTGTTTCCGt > 1:159964/1‑143 (MQ=255) ggTACGCGAGCTGTTTAGCGCCGTACGGAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATAACGTTTTCGGTTTGTTATTGCTGTTTCAGTCAATTGTTGCCg > 2:534179/1‑143 (MQ=255) ggTACGCGAGCTGTTTAGCGCCGTACGGAAAAACTATTTCCGCCTCTAGTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATAACGTTTTCGGTTTGTTCTTGCTGTTTCCGTCAATTGTTGCCg < 2:207268/143‑1 (MQ=255) tttAGCGCCGTACGGAAAAACTATTTCCGCCTCTATTTTCACCATATGTCTTTCAACATCGCCCGCATTCTCCATTTGCAGGTCGATAACGTTTTCGGTTTGTTCTTGCTGTTTCCGTCAATTGTCGCCGGtacgcttacgct > 1:449434/1‑143 (MQ=255) accGTACGGAAAAACTATTACCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATAACGTTTTCGGTTTGTTCTTGCTGTTTCCGTCAATTGTTGCCGGTACGATTACGCTCGGCCt < 1:249233/142‑1 (MQ=255) gTACGGAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATAACGTTTTCGGTTTGTTCTTGCTGTTTCCGTCAATTGTTGCCGGTACGATTACGCTCGGCCtgat > 1:316130/1‑143 (MQ=255) gAAAAACTATTACCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCCCTATTTGCTGGTCGATAACGTTTTCGGTTTGTTCTTGCTGTTTCCGTCAATTGTTGCCGGTACGATTACGCTCGGCCTGATGACGc > 1:342239/1‑143 (MQ=255) aaaaaCTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATAACGTTTTCGGTTTGTTCTTGCTGTTTCCGTCAATTGTTGCCGGTACGATTACGCTCCGCCTGATGTCGcg > 1:86170/1‑142 (MQ=255) aaccacttCCGCCTCTTTTTTCACTTTATGTATTTCTACCTCTCCCGCGTTCTCTATTTGCAGGTCGATAACGTTTTCGGTTTGTTCTTGCTGTTTCCGTCAATTGTTGCCGGTACGATTACGCTCGGCCTGATGACGCAGAt < 2:309743/137‑1 (MQ=255) aaCTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATAACGTTTTCGGTTTGTTCTTGCTGTTTCCGTCAATTGTTGCCGGTACGCTTCCGCTCGGCCTCATGCCGCAGAt > 2:595869/1‑143 (MQ=255) cTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATGACGTTTTCGGTTTGTTCTTGCTGTTTCCGTCAATTGTTGCCGGTACGATTACGCCCGGCCTGCTGACGCAGAttg > 2:124544/1‑142 (MQ=255) tctATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATAACGTTTTCGGTTTGTTCTTGCTGTTTCCGTCAATTGTTGCCGGTACGATTACGCTCGGCCTGATGACGCAGATTACCAACGttttt > 1:155723/1‑143 (MQ=255) ctATTTTCCCTATCGGTCTTTCAGCATCGCCCGCATTGTCTTTTTGCAGGTCGATAACGTTGTCGGTTTGTTCTTGCTGTTTCCGTCAATTGTTGCCGGTACGATTACGCTCGGCCTGATGACGCAGATTACCAACGTTTTTg < 2:311631/143‑1 (MQ=255) ctATTTTCACTATATGTAGTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATAACGTTTTCGGTTTGTTCTTGCTGTTTCCGTCAATTGTTGCCGGTACGATTACGCTCGGCCTGATGACGCAGATTACCAACGTTTTTg < 1:43670/143‑1 (MQ=255) | GCCGGGGCTGGAGTTTAAAAACCAGCGTGTAGAGGCTGCCTACCGTAAAGAGCTGGTTTATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATAACGTTTTCGGTTTGTTCTTGCTGTTTCCGTCAATTGTTGCCGGTACGATTACGCTCGGCCTGATGACGCAGATTACCAACGTTTTTG > NC_000913/397433‑397716 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |