| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 696,470 | C→T | 100% | noncoding (35/75 nt) | glnX ← | tRNA‑Gln |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 696,470 | 0 | C | T | 98.9% | 90.2 / ‑6.5 | 39 | noncoding (35/75 nt) | glnX | tRNA‑Gln |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (13/24); minor base G (0/1); total (14/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCT > NC_000913/696330‑696603 | cGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCACGCAATTGCGGAATTAg > 2:397738/1‑143 (MQ=255) ggCATCCGACATTGAAGGGTAAGGCGTGTCAACATCGCATTCGACATTGAATGAGCGCAGAAAAGCAAGAAGCTCGTCGTAGCGAGCTTGTGTAATGTGGCTGGGGTACGGGGATTCGAACCTCGGAATGCCGGAATTAGAAt < 2:452693/142‑1 (MQ=37) gCATCCGACATTGAAGGATAAGACGTGTCAACATCGTCTCCAACATTTAATGAACGCCGATAAGCGATGAGCTCGCCGAAGCGAGCTTTTTTAATGTTGCTGTGGTACGAGTATTCGAACCTCGGAATGCCGGAATTAGAATc < 1:601716/143‑1 (MQ=25) ctCCGACACTGAAGTATAAGACGTGTCAACATCCCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCg < 1:108608/142‑1 (MQ=255) aTCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGCTATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGACCCTCCGAATGCCGGAATCAGAAGCCg > 2:480386/1‑143 (MQ=39) gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGc < 1:171130/143‑1 (MQ=255) cATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCt > 2:252938/1‑143 (MQ=255) aaGGATTAGACTTGTCAACATCGCATTCGACATTGAATGTACGCAGTAAACCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGTTGTGGTACTAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCg < 2:620664/143‑1 (MQ=37) aaCATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaa < 2:499350/143‑1 (MQ=255) aaCATCGCATTCGACATTGAATGAACACAAAACAGCGAAAAGCTCGCCGACGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaa > 1:642505/1‑143 (MQ=21) cATCGCGTTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACt < 1:194/143‑1 (MQ=255) cATCGCATTCGACCTGGAGTGATCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCGTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCGCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACt < 1:19024/143‑1 (MQ=21) cATCGCATTCGACATTGAATGAACGAAGAACAGCAAAAAGCGCTCTGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACt > 1:345877/1‑143 (MQ=21) cATCGCATGCGACATCGAATGAAGCCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTTGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAAACAGAGCCTTACCGCTTGGCGATACCCCAACt < 2:590689/143‑1 (MQ=21) tCGCATCCGGCGTTGAATGAACGCATAAAAGCAAAAAGCTCGCCGTTGCGAGCTTTTTTCCTGGGTCTGGAGTACGAGGATTCGAACCTCGGAAGGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg < 2:320707/143‑1 (MQ=18) cGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGCATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGCGCACt > 2:103841/1‑143 (MQ=255) cGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt > 1:371506/1‑143 (MQ=255) tgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTaa < 2:556858/143‑1 (MQ=255) gaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGC‑TTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGc < 1:23166/143‑1 (MQ=255) aaCGCAGAAAAGCAAACTGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGc < 2:72967/143‑1 (MQ=255) aGAAAAGCAAAACGCGCGCCGAAGCCAGCTTTGTTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt < 2:472690/143‑1 (MQ=21) aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATGCGGTGCCTTACGGCTTGGCGATACCCGAACTGGGGGCACGTACAAGGTAAGCgccgtgt > 2:447091/1‑137 (MQ=21) aGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCCCGTACAAGGCATGCGTCTTGactc > 1:499351/1‑140 (MQ=21) aacaGCTCGCCTAAGCGAGCTGTTTTAATGTTGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAAGTAGATTCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAtt < 2:336661/140‑1 (MQ=25) gaggCTCGCCGAAGCGAGCTTGTTTAAGGTGGCGGGGGGACGAGGATTCGAACCGCGGAATGCCGGAATTAGAAGCCGGTGCCTGACCGCGTGGCGATAGCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTg < 2:197832/140‑1 (MQ=255) aaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTCAGCGTCTTGAATAAATTg > 1:501964/1‑143 (MQ=255) aGCCCGCCGCGGCGAGCTTTTTTAGTGCGGCTGGGGTACGAGGATTCGAACCTCGGAGTGCCGGAATTAGAAGCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGc < 2:391643/143‑1 (MQ=21) cgcgGAAGCGAGCTTTTTTAATGTGGCTGGGGGACGGGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTggg < 2:149662/143‑1 (MQ=255) cGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCAGAATTAGAATCCGGTGACTTACCGCTTGGCGATACCCCAACTGGGGGCACTTACAAGGGAAGCGTATTGAATAAATTGGCTCGGGTa > 1:63368/1‑143 (MQ=21) ttttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCg > 1:465329/1‑143 (MQ=255) ttttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTAAAGGGGAAGCGCTTTGAATAAATTGGCGGGGGTACggggctacg > 1:468585/1‑138 (MQ=18) tttttAGTGTCGCGGGGGTACGAGGATGCTGCCCTCGGAATGCCTGAAGTAGAATCCGGTGGCTGATCGCTTGTCTATACCCCACGTGGGTGCACTGACAACGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAtttaa < 2:277504/143‑4 (MQ=255) tgggaaggtgGCGGGGGTACGAGGATTCGAACCTCGGAATGGCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGaa < 1:480386/136‑1 (MQ=25) tGGGGTACGAGGATGCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGcc < 1:252938/143‑1 (MQ=255) gTACCAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGaa < 1:447091/143‑1 (MQ=255) cGAGGGTTCGAGCCGCGGAATGCGGGAATTAGAATCCCGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGGCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCa < 2:193237/143‑1 (MQ=37) aCCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc < 1:192317/143‑1 (MQ=255) cgggaggCCGGGATGAGAAAGCGGCGCCTTACAGCTTGGCGATACCCCGACGGGGTGCACGTACGAGGTAGGCGGCTTGATTAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTa < 2:30854/137‑1 (MQ=18) tGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAATTAAAAGGCCAGCGAGTAGAATACGTAGGCTGAGGTACGAGGCTACGAACCTGGGCGCGCCGGAAACCGAATCCGGAGCCTAACCgag > 2:524865/1‑141 (MQ=12) | CGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCT > NC_000913/696330‑696603 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |