| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 710,436 | G→A | 100% | H71Y (CAC→TAC) | fur ← | DNA‑binding transcriptional dual regulator Fur |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 710,436 | 0 | G | A | 100.0% | 90.9 / ‑6.7 | 33 | H71Y (CAC→TAC) | fur | DNA‑binding transcriptional dual regulator Fur |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (15/17); minor base C (1/0); total (16/17) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTTTGCGGCAATTTCACGCTGACGCGCTTCGATGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGA > NC_000913/710296‑710572 | ttttGCGGCAATTTCTCGCTGACGCGCTTCGATGGAATCATCACTAAATGCGATAACCTTGCCGCAGTAGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGc < 2:119325/143‑1 (MQ=255) ggCAATTTCACGCTGACGCGCTTCGATGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAACTGTCGCGGGTGa > 1:645873/1‑143 (MQ=255) gCAATTTCACGCTGACGCGCTTCGATGGAATCATCACTAAATCCGATACCCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGAc < 1:327089/143‑1 (MQ=255) tttCACGCTGACGCGCTTCGATGGAATCATCACTAAATTCGATAACCTTGCCGCCGTCGAGGCAGATCACGTGATCGTGGTGATGTTGCTGTGTCAGTTCACATACGGATTTCCCCCCTTCAAAATTGTAGCCGGTGACGATa > 1:249969/1‑143 (MQ=255) tCACGCTGACGCGCTTCGATGGGATCATCACTAAATGCGATGACCTTGCGGCAGTCGGGGCAGATCAGGTGATCGTGGTGATGTGGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGGGACGATAcc > 1:496929/1‑143 (MQ=255) aCGCTGACGCGCTTCGATGGAATCATCACTAAATTCGCTAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAg < 2:44506/143‑1 (MQ=255) aCGCTGACGCGCTTCGATGGAATCATCACTAAATTCGATAACCTTGCGGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTAAGTTCAACTAGGGATTTACCGTCGTCAAAAGTGTAGCGGGTGGCGATACCAg > 2:606527/1‑143 (MQ=255) gcgcTTCGATGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCa > 1:198172/1‑143 (MQ=255) ttCGCTGTAATCATCACTAAATTCGATACCCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACt < 1:145588/143‑1 (MQ=255) cGATGGAATCATCACTAAATTCGATAACCTTGGCGCAGTCGAGGCAGATCTGGCCCTTCGCGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑gg < 1:490210/143‑1 (MQ=255) cGATGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑gg < 1:574322/143‑1 (MQ=255) aTGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGtt > 1:459937/1‑143 (MQ=255) ggATTCATCATTAAATTCGATAACCTTGCCGTAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTCCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCa < 2:338101/143‑1 (MQ=255) ggAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCa < 1:256763/143‑1 (MQ=255) aTAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGAAGATACCAGCGTCGTCAAACT‑GGTTCACGACGCGATATACCGTAGc > 2:247102/1‑143 (MQ=255) agctttGCCGCAGTCGCGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCa < 2:604845/139‑1 (MQ=255) cTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGAc > 1:215614/1‑143 (MQ=255) cGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATAACAGCGTCGTAAAACT‑GGTTCAGTACGCGCTATACCGTAGCCAGACCaaaa > 2:88714/1‑141 (MQ=255) gATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAACATTGTAGCGGGTGACGATACCAGCGTCGTCAAACA‑GGTTCAGTACGCGATATACCGTAGCCCGACCGATTTCTTCACCCatat > 2:127051/1‑143 (MQ=255) gTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGCTCa > 1:574500/1‑143 (MQ=255) cggggtgATGTTGTTGTGTCAGTTCACATACGGGTTTACCGCCTTCAAACTTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGt < 2:361709/140‑1 (MQ=255) gATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGtat > 1:325881/1‑143 (MQ=255) gCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑AGTTCAGTACGCGATATACCGGAGGCAGACCAATTTCTTCACCGATAGCGCGCAGACGCTAGCATaaagat > 2:63951/1‑140 (MQ=255) gtgtAAGTTCAAATAGGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATAGATCAGACGTTTGTATAAATCTTcc < 1:156100/143‑1 (MQ=255) aaaGACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGt < 1:106277/143‑1 (MQ=255) aTACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGa < 2:198172/143‑1 (MQ=255) cGGATTTAGCGCCTTCAATATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATgg < 2:392868/143‑1 (MQ=255) ggATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGt > 2:496196/1‑143 (MQ=255) taTTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGtt < 2:215614/142‑1 (MQ=255) cGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGc < 2:459937/143‑1 (MQ=255) gCCTTCCAAATGGTAGCGGGTGACGATACCCGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCt < 1:198815/143‑1 (MQ=255) aaaaTTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTgac > 1:469905/1‑142 (MQ=255) aaTTGTAGCGGGTGACGATACCCGCCT‑ATCAAAATACGTACAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGAGGGTTGTCCGGCTCCTgagga > 2:205894/1‑143 (MQ=255) | TTTTGCGGCAATTTCACGCTGACGCGCTTCGATGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGA > NC_000913/710296‑710572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |