| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,637,646 | +T | coding (5/1500 nt) | pitA → | metal phosphate:H(+) symporter PitA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,637,645 | 1 | . | T | 96.4% | 168.7 / ‑0.3 | 57 | coding (4/1500 nt) | pitA | metal phosphate:H(+) symporter PitA |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base T (22/32); total (23/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
AATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GC‑TACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGC > NC_000913/3637504‑3637781 | aaTTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCAGGGCCTCAAAGTGGCGTACAGTGCTAT‑GC‑t > 1:1094078/1‑143 (MQ=255) agaaGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACAtt < 1:254400/141‑1 (MQ=255) aGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACAtttgt < 1:776934/143‑1 (MQ=255) aTAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTCCGTCGCCTCAGAGTGGCGTACCGTCCTAT‑GCTTACAtttgttt > 1:860778/1‑143 (MQ=255) tAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACAtttgtttg < 1:1132848/143‑1 (MQ=255) attTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGc > 1:719056/1‑143 (MQ=255) ttttttGAGTGAAATCCATACAGTCGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGcc < 2:823909/143‑1 (MQ=255) tttttGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTTCCCGTTCCGTGTAATTCACTGTTAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCt < 1:399755/143‑1 (MQ=255) ttttGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTg > 2:254714/1‑143 (MQ=255) ttttGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTg > 1:965817/1‑143 (MQ=255) ttGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCGATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGa > 1:802316/1‑143 (MQ=255) tGAAATCCATTCAGTGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGc < 2:245206/143‑1 (MQ=255) tGAAATCCATCCAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGCTCATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTACTAT‑GCTTACACATGTTTGCTGGCCTGCATATGc > 2:864761/1‑143 (MQ=255) tGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGc < 2:395478/143‑1 (MQ=255) tGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTAGCCTGGCTTTGc > 2:981366/1‑143 (MQ=255) gAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCTGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCa < 1:800101/143‑1 (MQ=255) atccatccAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCACGCGCCGCAAAAGAAACTGCTAATGCGCCGCGTTCATGTAATCAAAATGGCGTAAAGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGCTTTGCATAc > 1:840925/1‑143 (MQ=255) ggggCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCATTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGtta < 1:298626/143‑1 (MQ=255) gCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCATCAAAATGGCGTAACGTCCTAT‑GCTTACATTGGTTTGCTGGCCTGGCTTTGCATACCGGGCTGttcttc > 1:1163360/1‑142 (MQ=255) aaTCAAAAAAAGTCTATATTTCACTTCGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttg < 2:208695/143‑1 (MQ=255) aTCAAAAAAAGTCTATATTTCACTTGGGCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCGCAAAATGGCGTAACGTCCTAT‑GC‑TACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgct < 1:828736/143‑1 (MQ=255) tCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCGTGGATTTGCATACCGGGCTGTTATTAttgct > 1:130313/1‑143 (MQ=255) cAAAAAAAGTCTAGAGTTCACTTTGCCCGCGCCGCGAAAGTCGCTGATAGGGCGCCGCGTTCATGTCCTCAAAATGGCGTATCGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGTTTTGCATACCGGGCTGTTATTAttgctt < 2:370605/143‑1 (MQ=255) aaaaaaGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgcttgc > 2:1125792/1‑143 (MQ=255) aGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTgg < 1:532718/143‑1 (MQ=255) gTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGc > 1:1164224/1‑143 (MQ=255) tCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGCTATTATTGCTTGCACTGGCt > 2:346133/1‑143 (MQ=255) cTATATTTCACTTTGCCTGCCCCGCGAAATTCACTGATACTGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCTGGCTGTTATTATTGCTTGCACTGGCtt < 1:204745/143‑1 (MQ=255) cTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCCAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCtt < 1:165797/143‑1 (MQ=255) atatTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCtttt < 1:836289/143‑1 (MQ=255) atTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtgt < 1:881683/143‑1 (MQ=255) tttCACTTTGCCCGCGCCGCGAACGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtgtg < 2:421572/143‑1 (MQ=255) gttgaCTTTGCTTGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtgtg < 2:414279/139‑1 (MQ=255) cttCACTTTGCGCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCCTGTCCTCAAAATGGCGTAACGTCCTAT‑GCGTCCATTCGTCTGCTGGCCTGGATTTGCCTACCGGGCGGTTATTCGTGCTTGAACCGGCcttgttg > 2:832260/2‑139 (MQ=255) cACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTg < 1:718943/143‑1 (MQ=255) cccGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACg > 1:108956/1‑143 (MQ=255) cccGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATGATTGCTTCCACTGGCTTTTGTGCTGGTcggtg > 2:304806/1‑139 (MQ=255) ccgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGa > 1:1070547/1‑143 (MQ=255) ccgcgAACGTCCCTGATATTGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCa < 2:460854/143‑1 (MQ=255) aaaGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAAt < 1:623582/143‑1 (MQ=255) acggATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTc < 1:506813/140‑1 (MQ=255) cTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTcc < 1:453319/143‑1 (MQ=255) gATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCAt < 1:96598/143‑1 (MQ=255) tgaTGCTCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGa < 1:522044/141‑1 (MQ=255) tAATGCGCCGCGTTCATGTCCTCCAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGTTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGa < 1:101730/143‑1 (MQ=255) gccgcGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGc > 1:319152/1‑143 (MQ=255) gccgcGGTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTTTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGc < 1:350998/143‑1 (MQ=255) cgcgTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCa < 2:108956/143‑1 (MQ=255) gcgTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCaa < 1:254714/143‑1 (MQ=255) aTGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGt > 1:372336/1‑143 (MQ=255) tGTCCTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGTCGTCAATTGTTGCCATGACACAGCCAGCGCCGTg > 2:976295/1‑143 (MQ=255) cTCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCaa > 1:754355/1‑143 (MQ=255) tCAAAATGGCGTAACGTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAAc > 1:319228/1‑143 (MQ=255) cgcgCGTACCGTTCTATGGT‑TTCATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACCAAGCCATCAATGTTTTCCATGACACAGCCAACGCCGTGGCAACCGTTa < 2:821848/140‑1 (MQ=255) tGGCGTAACGCCCTAT‑GCTTACATTTGTTTGCTGGCCTGGCTTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCAGGACACAGCCAACGCCGTGGCAACCGTTAt < 2:102818/143‑1 (MQ=255) gTCCTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCcg > 2:732886/1‑143 (MQ=255) tcttAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTGGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCcgc < 2:693363/140‑1 (MQ=255) cTAT‑GCTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCACCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCcgcgc < 1:180073/143‑1 (MQ=255) | AATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GC‑TACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGC > NC_000913/3637504‑3637781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |