| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 125,387 | C→T | Q791* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,387 | 0 | C | T | 98.0% | 139.7 / NA | 51 | Q791* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (19/30); minor base G (1/0); total (20/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAA > NC_000913/125247‑125526 | tcaTCCTTGCGCTAGTTTTCGGCTTAGGTTCTTTCGTTTATATCTTTACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAg < 1:119432/141‑1 (MQ=255) cTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTGAGGtgag > 1:805668/1‑142 (MQ=255) ggCGAAAGATGATGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCAACGAGCTGCCGCTTGATGGTCAGGATTGTGAACGCTGGAACATTCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGtgatga < 2:1098886/143‑1 (MQ=255) aaaGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAacga < 1:670206/143‑1 (MQ=255) aaGATTACGTCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAacgac > 1:807070/1‑143 (MQ=255) aaGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAacgac > 1:848885/1‑143 (MQ=255) aGATTGCGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAacgacg < 2:498143/143‑1 (MQ=255) aGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAacgacg < 2:494573/143‑1 (MQ=255) gCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCa > 1:702687/1‑143 (MQ=255) gTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGt < 1:19914/143‑1 (MQ=255) gTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGGGAGGTGCGGTGGTCCCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGa > 1:1256443/1‑143 (MQ=255) tttATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGAc > 2:1093695/1‑143 (MQ=255) tttATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGAc < 1:426660/143‑1 (MQ=255) tttATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGCGGCATCTACCGAc > 1:244176/1‑143 (MQ=255) ttATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGCCAGTGGCACCTACCggcg > 1:297628/1‑140 (MQ=255) ataGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGGATATCGCTTCGGTGCTGAACGACGCTCCGGCAGTGGCATCTACCGACtct > 2:473228/1‑141 (MQ=255) taGCGTGACCTCCTTCAACGAGCTGGCGCGGGATGGGCAGGAGTGGGCGCGCTGGCCCATGCTGCACCCGGTGGAAACTCCGCGCGTGCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCAGCTACCGACtata < 1:243784/143‑1 (MQ=255) cGTGACGTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAGCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGa < 2:173261/143‑1 (MQ=255) tcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGtt < 2:250231/143‑1 (MQ=255) cTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGGATATCGCGTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGt > 1:1039153/1‑143 (MQ=255) gcgcGTGATGGTCAGGATTGTGATCGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCGTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCg < 2:1128198/143‑1 (MQ=255) gcgcGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACGGTATGAGACTGCTCGCTGAGCAGGGCCg > 2:933/1‑143 (MQ=255) gcgTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTACgac > 1:279928/1‑141 (MQ=255) gcgTGATGGCCAGGATCGTGACCGCCGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATAGCGCTTAGGTGATGACCGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTa < 1:1213088/143‑1 (MQ=255) gATGGTCAGGAGTGTGAACGCTGGAACAGGCTGCGCCCGCTGGAAACTCCGCGCGGTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTa < 2:319909/143‑1 (MQ=255) tGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACg < 1:1236689/143‑1 (MQ=255) tCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAGCGACGCGCCGGCAGTGGCATCTACCGACTGTGTGGAACGATCCGCTGAGCGGGTCCGTTCTCACGTAc > 2:311149/1‑143 (MQ=255) aGGATTGTGACGGCTGGAACATGCTGCACGCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCg < 2:124788/143‑1 (MQ=255) aGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCg < 1:915437/143‑1 (MQ=255) aTTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTGCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGCGGCATCTACCGACTATATGAAACTGTCCGCTGAGCAGCTCCCTACTTACGTACCgaat > 2:877429/1‑140 (MQ=255) gtgAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGCCGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTTCCGTCTtac > 1:259376/1‑143 (MQ=255) tgctgcCCCCGCTGGAAACTCCGCTCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAACCTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTg < 1:51835/143‑1 (MQ=255) gctgcACCCGCTGGAACCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGCCAGTGTCTTCTACTGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTgg < 1:990081/143‑1 (MQ=255) gctgcACCCGCTGGAACCCCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAACCTGTGCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTgg < 1:740746/143‑1 (MQ=255) ctgcACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGCCTACCGCGTACTggg > 1:765711/1‑143 (MQ=255) cGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGAt < 1:1144555/143‑1 (MQ=255) cGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGAt < 2:864417/143‑1 (MQ=255) gCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTCCTGGGTACTGATg < 2:702687/143‑1 (MQ=255) cgggACACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGGACCGGCTGACGACTACCGCGTACTGGGTACTGATgg < 1:819860/141‑1 (MQ=255) ggAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTGTATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCt > 1:475086/1‑143 (MQ=255) aaCTCCGCGCTTTCCTTATATCGCTTAGGTGATGCACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCg < 2:119645/143‑1 (MQ=255) aaCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCg < 1:1093695/143‑1 (MQ=255) ccGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCg > 2:1259609/1‑143 (MQ=255) gcgcgTTCGGTAGCTGCCTCAGGGGATGATCGACGCTCCGGCAGTCGCAGCTACCGACTATATGACACGGGCCGCTGAGCAGGTCCGTACTTACGTACCGGCTGCCGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGtt < 2:965135/143‑1 (MQ=255) gcgcgTTCCGTATATCTCTTAGCGGATGAACGACGCTCCCGCAGTGGCATCTACCGACTATATGAAACCGTTCGCTGAGCTGGTCCGCACCTACGTACCGGCTGACGACGTCCCCGTACCCGGTACACATAGCTGCGGCCgct > 2:761224/1‑141 (MQ=255) gTTCCGTATAGCTCTTAGGTTATGAACGGCGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGa < 2:48419/143‑1 (MQ=255) ttCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGAGGGCTTCGGTCGTTCCGAc > 1:304085/1‑143 (MQ=255) tCCGTATATCGCTTAGTTGTTGAATGACGTTCCGGCTGTGGCATCTAGCGACTATATGAAGCTGTTCGCTGAGGAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACa < 1:538464/143‑1 (MQ=255) ccGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAg < 1:1140015/143‑1 (MQ=255) atatCGCCTAGGTCATGTACGACGCTCCGGTGGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGGACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGt < 2:289927/143‑1 (MQ=255) gCTTAGTTGATGAACGACGCTCCGGCAGTGGCAGCATGCGACTATATAAAACTTTGCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGaa < 1:346916/143‑1 (MQ=255) | AGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGAA > NC_000913/125247‑125526 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |