Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,661,969	C→G	V55L (GTT→CTT)	iscR ←	DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,661,969	0	C	G	100.0%	210.8 / NA	67	V55L (GTT→CTT)	iscR	DNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (39/28);  total (39/28)

GCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATA  >  NC_000913/2661829‑2662107                                                                                                                                             |                                                                                                                                           gccgccCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGACCACGTACGCTGGAAAGCa                                                                                                                                          >  1:683146/1‑143 (MQ=255)  ccgccCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAg                                                                                                                                         >  1:1049976/1‑143 (MQ=255)  ccgccCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGCACGCTGGAcagcag                                                                                                                                         >  2:1168193/1‑143 (MQ=255)   cgccCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGa                                                                                                                                        <  2:1005205/143‑1 (MQ=255)    gccCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGAc                                                                                                                                       >  2:349839/1‑143 (MQ=255)     cccTGGCAGCCGCCTTTACCCTGACACCGGGTGGCATCTACAGATTCGTCAACGGCGCCAATTACTTCGCCAACGGCGATGCTGCTGGCACCTTTGCCTAACAGATAACCACCGCCTGCTCCACGTACGCCCGAACGCAGAcc                                                                                                                                      >  2:589305/1‑143 (MQ=255)       cTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTGACAGATAACCACCGCCTGGTCGACGTACGCGGGAAAGCAGAcaga                                                                                                                                    >  2:991633/1‑140 (MQ=255)        tGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAtt                                                                                                                                   <  2:916284/143‑1 (MQ=255)        tGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAtt                                                                                                                                   <  1:1141712/143‑1 (MQ=255)        tGGCAGCCGCCTTTACCCTGACAACAGGTGGCATCTACACCTTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACACATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCagt                                                                                                                                   >  2:22551/1‑141 (MQ=255)         ggCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAttt                                                                                                                                  >  1:952302/1‑143 (MQ=255)          gCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAtttt                                                                                                                                 >  1:11531/1‑143 (MQ=255)              ccgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACg                                                                                                                             <  1:381788/143‑1 (MQ=255)                      aCCCCGACAACGGGTGGCATCTGAGGATTCGGCAAGGGCGCTAATTACTTCGCCAACGGGGATGCTGCTGGCATCTTGGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACggg                                                                                                                     <  2:1029945/143‑1 (MQ=255)                        ccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGaa                                                                                                                   >  1:772523/1‑143 (MQ=255)                         cTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGATGCTCACGTCGCGTCCTAACGGATAATCACCGCCTTGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGaaa                                                                                                                  <  1:929592/143‑1 (MQ=255)                          tGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCGCCGTGCGCGTGAATGCAGACCATTTTTACGCAGACGGGaaaa                                                                                                                 >  1:859856/1‑143 (MQ=255)                                  ggTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAgttgtt                                                                                                         >  1:477650/1‑143 (MQ=255)                                     ggCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCa                                                                                                      <  2:400490/143‑1 (MQ=255)                                        aTCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGAt                                                                                                   <  1:1074612/143‑1 (MQ=255)                                           tACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAg                                                                                                >  2:855379/1‑143 (MQ=255)                                            aCAGATTCGTCAACGGCGCTAATGACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGa                                                                                               <  2:1072082/143‑1 (MQ=255)                                                    gTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGCAAACAGTTGTTCCAGCTAAGAAGGTGaaa                                                                                       >  2:1223758/1‑143 (MQ=255)                                                    gTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGaaa                                                                                       <  1:699850/143‑1 (MQ=255)                                                     tCAACGGCGCTAATTACTTCGCCCACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAAGCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCACGCGGGAAAACCGTTGTTCCAGAACAGAAAGGGAAAt                                                                                      >  2:1064860/1‑143 (MQ=255)                                                     tCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACAGGCTGGTCCACGTACGCTGGAAAGCAGACCATTCTAACGCAGACGCGAAACCAGATGTGCCCGATCACACAGGGaacg                                                                                      >  2:749793/1‑141 (MQ=255)                                                        ccGGGGCCAATTCCGTCGCCACGGTCGATGCTGCTGGCGTCTTTGCCTAACAGATAACTACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTcc                                                                                   <  2:685168/142‑1 (MQ=255)                                                         cGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTAc                                                                                                                              >  2:419120/1‑99 (MQ=255)                                                         cGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTAc                                                                                                                              <  1:419120/99‑1 (MQ=255)                                                          ggCGCTACTTACTTCGCCACCGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCt                                                                                 <  1:624877/143‑1 (MQ=255)                                                          gccgcTAATTACTGCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGAACATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCt                                                                                 <  2:408116/141‑1 (MQ=255)                                                            cgcTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATCACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAACACAGTCGTTCCAGACAAGAAAGAGAACTTCCCTGa                                                                               >  2:732265/1‑143 (MQ=255)                                                               tAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGCAGACAGTTGTTCCAGATACGCAAGGGAAATCTCCTGACgg                                                                            >  2:1007547/1‑142 (MQ=255)                                                                  taaCTGCGCCAACTGCGATGCTGCTGGCATCTTTGCCTAAAAGATAACCACCGCCTGGTCCACGTACGCGGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCg                                                                         <  1:577346/141‑1 (MQ=255)                                                                   tACTTCGCGAACGGCGATGCTGCTCGCATCTGTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCgg                                                                        <  2:116984/143‑1 (MQ=255)                                                                      ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCGGATAAGAAGGGGAAATTCCCGGGCGTTCGGaaa                                                                     >  1:379343/1‑143 (MQ=255)                                                                      ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa                                                                     >  1:730327/1‑143 (MQ=255)                                                                             aCGGCTATGCGGCTGGCATCTTTGGCTAACGTATCACCTCCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCTGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGc                                                              <  2:179600/143‑1 (MQ=255)                                                                                  gATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTGCACGTACGCTGGAAAGACGACCATTTTTACGCAGACGGGAACACAGTTGTTCAAGCTAAGAAAGGGAAATTCCCTGACGTTCGGAGATGTCAGCCAACg                                                         >  2:590501/1‑143 (MQ=255)                                                                                  gATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAACTATCACCCAACg                                                         >  1:463671/1‑143 (MQ=255)                                                                                  gATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACg                                                         >  1:235278/1‑143 (MQ=255)                                                                                   aTGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACgg                                                        >  2:1253350/1‑143 (MQ=255)                                                                                          tgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAACACAGTTGCTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCgcg                                                 >  1:1268421/1‑141 (MQ=255)                                                                                           tgCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGc                                                <  1:450903/142‑1 (MQ=255)                                                                                             catcatTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGccc                                              <  1:629725/143‑1 (MQ=255)                                                                                               tCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGc                                            >  1:203799/1‑143 (MQ=255)                                                                                               tCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGc                                            >  1:383450/1‑143 (MQ=255)                                                                                               tCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGc                                            <  1:855379/143‑1 (MQ=255)                                                                                                 tttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCtt                                          <  1:48955/143‑1 (MQ=255)                                                                                                 tttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCtt                                          >  1:576941/1‑143 (MQ=255)                                                                                                 gttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCtt                                          <  2:1085676/142‑1 (MQ=255)                                                                                                   tGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCa                                        <  2:1094442/143‑1 (MQ=255)                                                                                                    gCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCag                                       >  2:585233/1‑143 (MQ=255)                                                                                                     ccTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGGTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTCCCGGGCCCGCTACaga                                      >  2:1017500/1‑143 (MQ=255)                                                                                                        aaCAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGtt                                   <  2:576941/143‑1 (MQ=255)                                                                                                         aCAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGtgg                                  >  1:1000830/1‑141 (MQ=255)                                                                                                           ataTAACCACCGCCTGGGCAACGTACACTGGAAAGCAGACCATTTTTGCGCAGACGGGAAAACAGTTGTTCCAGATACGACAGGGAAATTCCCTGACGTTCGGAAATAGCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAg                                <  2:284634/141‑1 (MQ=255)                                                                                                            gATAACCACCGCCCGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgc                               <  1:259074/143‑1 (MQ=255)                                                                                                                       ccTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTAACGGCCCCGCCTCAGAGTTGAGCACAACCTCGAg                    >  2:11254/1‑143 (MQ=255)                                                                                                                       ccTGGTCCACGTACGCTGGAAAGCAGACCATTATTACGCAGACGGGAAAACAGTTGTTGCAGATAAGAAAGGGAAATTCACTGACGTTCGGAGATATCAGCCAACGGTACGGGGCAGGCTTCAGGAACGAGCGCAACGCCacg                    >  2:705372/1‑141 (MQ=255)                                                                                                                            tcccggTACTCGGGAGAGCAGACCAGTTGTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAAGTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTg               <  2:518511/138‑1 (MQ=255)                                                                                                                             ccACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGc              >  1:218680/1‑143 (MQ=255)                                                                                                                              cACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCg             >  1:343241/1‑143 (MQ=255)                                                                                                                              cACGTACGCTGGAAAGCAGACCAGTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCg             <  2:1049976/143‑1 (MQ=255)                                                                                                                               aCGTACGCTGGAAAGCAGACCATGTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGGTGCGCGCAACGTCACGCAGTGCgg            >  1:597152/1‑143 (MQ=255)                                                                                                                                cGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGt           >  2:235942/1‑143 (MQ=255)                                                                                                                                       tGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATCCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCACTGCGGTCACGGCa    >  2:159234/1‑143 (MQ=255)                                                                                                                                         gAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATa  >  1:184827/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                           GCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATA  >  NC_000913/2661829‑2662107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑