Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,632	T→A	V107E (GTG→GAG)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,632	0	T	A	98.5%	192.2 / NA	67	V107E (GTG→GAG)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (29/37);  minor base C (1/0);  total (30/37)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCT  >  NC_000913/3041493‑3041767                                                                                                                                            |                                                                                                                                        gccCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGAc                                                                                                                                      >  2:597713/1‑143 (MQ=255)     aTTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATc                                                                                                                                  >  1:713981/1‑143 (MQ=255)      ttGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGAGACCAGCGGCTGACGGAACTGAAAAAATATGCGGTATTCTATAAAGAGACCATcg                                                                                                                                 >  2:610780/1‑143 (MQ=255)        gCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATcgcg                                                                                                                               <  2:25703/143‑1 (MQ=255)        gCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTCTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATcgcg                                                                                                                               <  1:1006190/143‑1 (MQ=255)         cGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGCACTGAAACAATATGCGGTATTCTCTAAAGAGACCATCgcgc                                                                                                                              >  1:610928/1‑143 (MQ=255)          gACGGCAAAGGTAAAATGTGGCGCAATTTACGGCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGcc                                                                                                                             <  1:1062333/143‑1 (MQ=255)             gCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCACCAAAGAGACCATCGCGCCCga                                                                                                                          >  2:823331/1‑143 (MQ=255)               cAACGGGAAAAGGTGGAGCAATCTGCGTCTGTCCCGCGACGGCGATGGCTTTGCATGGGTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAgacg                                                                                                                        <  2:549391/143‑1 (MQ=255)                 aaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGCGACCCTCGCGGCAgccgac                                                                                                                      >  1:15648/1‑143 (MQ=255)                     tAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGc                                                                                                                  <  1:287231/143‑1 (MQ=255)                      aaaaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAACGAGACCATCGCGCCAGACGACGAGCg                                                                                                                 >  2:1079477/1‑143 (MQ=255)                           gtgGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGtgc                                                                                                            <  1:905022/143‑1 (MQ=255)                              gAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCCGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGtgctgc                                                                                                         >  2:1108159/1‑143 (MQ=255)                              gAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGtgcggc                                                                                                         >  1:394278/1‑143 (MQ=255)                              gAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGAGTGAACGGCGCAGCGTGCGTGAACCGCAGCTGGCTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGtgctgc                                                                                                         >  2:208702/1‑143 (MQ=255)                              gAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTGTGCATGGATTGAACGCCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGtcctgc                                                                                                         >  1:272389/1‑143 (MQ=255)                               agcgctttGCGGCTGTGCCGCGGCGGCGATGGCGTTGCATGGATTGAGGGGCGCAGCGTGCGTGAACCGTAGCGGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTgctgct                                                                                                        <  2:15366/138‑1 (MQ=255)                                 gattttACGTCTGTGCCGCGGCGGCGGTGGCGTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGCACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                                                                                      <  2:873160/140‑1 (MQ=255)                                   gtcgaCGTCTGTCGCGCGACGGCGATGGCTTGGCACGGATTGAACGGCGACGCGTGCGTGAAGCGCAGCTGACTGAGCTGAAAAAATATGCGGTATTCTCTGAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgt                                                                                                    <  2:442643/139‑1 (MQ=255)                                         gTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGcc                                                                                              >  2:727309/1‑143 (MQ=255)                                           cTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGGGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCgg                                                                                            <  1:513726/143‑1 (MQ=255)                                             gTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGtt                                                                                          <  1:824719/143‑1 (MQ=255)                                              ttCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAGATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTCCCGGatt                                                                                         >  2:884994/1‑143 (MQ=255)                                              ttCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGttt                                                                                         >  1:97623/1‑143 (MQ=255)                                                 cgcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCa                                                                                      <  1:891856/143‑1 (MQ=255)                                                 cgcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTGTTCa                                                                                      >  2:1101096/1‑143 (MQ=255)                                                    gACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATAGGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgc                                                                                   >  1:798016/1‑143 (MQ=255)                                                      cGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgc                                                                                 <  1:589118/143‑1 (MQ=255)                                                        gCGATGGCTTTTCATGGTTTGAACGCCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCGAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgc                                                                               <  2:528186/143‑1 (MQ=255)                                                          gATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGACAGAAGACGAGCGTGTGCTGCTTGGTGTTGCCGGTATTCAGgcgcgcgc                                                                             >  2:325589/1‑143 (MQ=255)                                                              gCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgcg                                                                         <  1:1267335/143‑1 (MQ=255)                                                               cTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgcgc                                                                        <  2:879562/143‑1 (MQ=255)                                                                gttGCATGGATGGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAGAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCt                                                                       <  2:234303/142‑1 (MQ=255)                                                                    gaTGGATTGAACCGCGCAGCGGGCTTGAAGCGTAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGATACCATCGCGCCATACTACTAGCGTGTGCTGCTTGGTTTTGCGGGTTTTCAGGCGCGCGCCGCGCTGGCa                                                                   <  1:356106/142‑1 (MQ=255)                                                                     aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaa                                                                  <  1:610780/143‑1 (MQ=255)                                                                       ggATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGGCGGGCTGGCAAAt                                                                >  1:32015/1‑143 (MQ=255)                                                                        gATTGAACGTCGCCGCGTGCGTGAACCGCAGCTCACTGACCTGAACAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtc                                                               <  1:600900/143‑1 (MQ=255)                                                                          ttGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtctc                                                             >  1:731846/1‑143 (MQ=255)                                                                           tGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCTGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct                                                            <  2:171032/143‑1 (MQ=255)                                                                           tGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCAGCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct                                                            <  1:597713/143‑1 (MQ=255)                                                                             aggggCGCGGGGGGCGGGGACCGCAGCTGACTGGACTGCCTGAATGGTTTGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCttt                                                          <  2:1228244/140‑1 (MQ=255)                                                                              aCGTCGCAGCGTGCGTGAAGCGCAGCTGACTGAACTTAAAAAATATGCGGTATTCTGTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa                                                         <  1:295877/143‑1 (MQ=255)                                                                                   gcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGaa                                                    <  1:535750/143‑1 (MQ=255)                                                                                       cgtgcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGc                                                >  1:80198/1‑143 (MQ=255)                                                                                          gcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCtt                                             >  2:853904/1‑143 (MQ=255)                                                                                            gtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTGTCTAAAGAGACCATCGCGCCAGACGACGCGCGTGTGCTGCTTGGTGTTGCCGGTATTCAGGCGCGCGCCGCGCTGGCAGATCTCTTTAGCGCACTGACTCCg                                           >  2:137112/1‑143 (MQ=255)                                                                                               aaCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa                                        <  2:1224730/143‑1 (MQ=255)                                                                                               aaCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa                                        <  1:719292/143‑1 (MQ=255)                                                                                               aaCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa                                        >  1:781537/1‑143 (MQ=255)                                                                                                  cGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCCGGCGCGCGCCGCGCTGGCAAGTCTCCTTAGCGCACTGCCTTCggaggag                                     >  1:211830/1‑137 (MQ=255)                                                                                                  cGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgc                                                                                   <  2:171078/97‑1 (MQ=255)                                                                                                  cGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgc                                                                                   >  1:171078/1‑97 (MQ=255)                                                                                                  cGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGAAGACGAGCGGGGGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGACCTGCCTTCgaaagaa                                     >  2:836162/1‑143 (MQ=255)                                                                                                  cGCAGCGCACTTAACTTAGAAAACATGTTGTATTCTCTAAAGAGGCCATCGCGCGAGACGACTAGCGTGTGCGGCTTTGTGTTGCCGTTTTGCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaagaa                                     <  2:110456/143‑1 (MQ=255)                                                                                                   gCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaagaaa                                    <  2:798016/143‑1 (MQ=255)                                                                                                           ctgaactgagAAAATATGCGGTATTCCCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTa                            <  1:1022576/143‑1 (MQ=255)                                                                                                           ctgaactgacAAACGATGCGGTAGTCTCTAAAGAGACCATCGCGCCAGACGTCGAGCGTGTGCTGCTTGGTGTTGCTGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTATAAAGAAAAACAGGTa                            <  2:676244/143‑1 (MQ=255)                                                                                                           ctgaactgaaAAAATCTGCGGTATTCGCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCCCTAAACAGAATTCTCTTCGAAAGAAAAACAGGTa                            <  2:1111101/143‑1 (MQ=255)                                                                                                           ctgaactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTa                            <  1:727309/143‑1 (MQ=255)                                                                                                           ctgaactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTCTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGCAAGAAAAACAGGTa                            >  2:593200/1‑143 (MQ=255)                                                                                                             gaactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGt                          <  2:48891/143‑1 (MQ=255)                                                                                                                  gaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCGTGGTGTGTGCGGTTGTCAGGCGCGCGCCGCGCGGGCAAATCTCTTTAGCGCACTGACTTCGAAGGAGAAACGGGTAGTTAaag                     >  2:799460/1‑143 (MQ=255)                                                                                                                    aacaaTATGCGGTATTCTCTAAACATCCCATCGCGCCAGACGCCGAGCGTGTGCTGCTTGGTGTTGCCGGTTTGCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaa                   <  1:340472/140‑1 (MQ=255)                                                                                                                           tgcgaaaTTCACGAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTAAGGCGCGCGCAGCGCTGGCAAATCTCTTTAGCAAACTACATAGGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGa            <  2:664039/137‑1 (MQ=255)                                                                                                                                tATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACt       >  1:1159472/1‑143 (MQ=255)                                                                                                                                     tctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCGAATCTCTCTAGCGAACTGCCTGCGAAAGCACAACAGGTCGTCAAAGAAGGCGCGAACACTCTGCt  >  2:855860/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                        GCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCT  >  NC_000913/3041493‑3041767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑