Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,168,472	G→A	intergenic (+25/‑169)	gltT → / → rrlB	tRNA‑Glu/23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,168,472	0	G	A	100.0%	19.4 / NA	13	intergenic (+25/‑169)	gltT/rrlB	tRNA‑Glu/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/9);  total (4/9)

TATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCG  >  NC_000913/4168341‑4168502                                                                                                                                    |                               tatCGCGCAACGCGTGATAAGCAATGTTCGTGGCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGGGAGTGAAAGTCACCTGCCTTAat                     <  2:237275/143‑1 (MQ=11) gatCACGCAACGCGTGATAAGCAATTTTCGTGTCCCATTCGTGTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAat                     <  1:405097/142‑1 (MQ=11)   tcgcggaaCGCGGGACAAGCAATTTTCGTGGCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGGAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAatat                   <  2:792366/137‑1 (MQ=11)   tcccGCAACGCGTGATAAGCAATTCTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAatat                   <  2:633804/140‑1 (MQ=11)     aCGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATAtct                 <  1:626845/143‑1 (MQ=255)     aCGCAACGCGTGATAAGCAATTTTCGTGCCCCCTGCGTCTAGAGGCCCAGGACACCGCCCTTTCCCGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATAtct                 <  2:589415/143‑1 (MQ=11)      cGCAACGCGTGATAAGCAATTGTCGTGTCCCCTTCGTCTAGCGGACCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATAtctc                <  1:347342/143‑1 (MQ=11)       gCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGCCACCGCCCTTTCACGGCGGTACCAGGGTTTCGAATCCCCTAGGGGACGCAAATTGCTGATTAGTGAGTGAAAGTAAACTGCCTTAATATCTCa               >  1:185252/1‑143 (MQ=11)       gCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCa               <  1:944846/143‑1 (MQ=255)         aaCGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCaaa             >  1:763574/1‑143 (MQ=12)                  tAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACAAAGCATTATCTCGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCtt    <  1:1200831/143‑1 (MQ=11)                    aGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCAGCTTCg  >  1:673035/1‑143 (MQ=255)                    aGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCGGCCTTAATATCTCAAAACTCATCTTCg  >  2:58842/1‑143 (MQ=255)                                                                                                                                    |                               TATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCG  >  NC_000913/4168341‑4168502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑