Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	97.9%	140.9 / ‑4.5	48	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (20/27);  total (21/27)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01

CGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGC  >  NC_000913/696333‑696607                                                                                                                                          |                                                                                                                                          cGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAt                                                                                                                                      >  2:581713/1‑143 (MQ=255)            ttGAAGGATAAGGCGTGGCAACAGCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTa                                                                                                                           <  2:1151881/143‑1 (MQ=255)              gAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAACTCGCCGAAGCGAGCTTTTTTAATATGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                                                                                         >  2:631401/1‑143 (MQ=255)                  gATAAGCCGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCtt                                                                                                                     <  2:202311/143‑1 (MQ=255)                  gATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCtt                                                                                                                     >  1:113345/1‑143 (MQ=255)                    tAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATGCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                                   <  2:694828/143‑1 (MQ=255)                    caaGACGTGTCAACAGCGCATTCGACATAGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                                   <  2:749697/142‑1 (MQ=255)                       gACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGa                                                                                                                >  1:324378/1‑143 (MQ=255)                          gtgtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAc                                                                                                             <  2:1182263/143‑1 (MQ=255)                          gtgtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTCGCGATAc                                                                                                             >  2:294844/1‑143 (MQ=255)                           tgtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAcc                                                                                                            <  2:373916/143‑1 (MQ=255)                               aaCATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATACGGTGCCTTACCGCTTGGCGATACCCCaa                                                                                                        >  1:230241/1‑143 (MQ=255)                                 cATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACt                                                                                                      <  1:448194/143‑1 (MQ=255)                                  aTCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGGCTTAGACACCGGTGCCTTACCGCTTGGCGATACGCCAACTg                                                                                                     >  2:261957/1‑143 (MQ=37)                                   tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCGTGGCGGTACCCCACCTgg                                                                                                    >  1:935247/1‑143 (MQ=255)                                    cGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTAGCGATACCCCAACTggg                                                                                                   >  1:949877/1‑143 (MQ=255)                                     gcgtgcGACATTGTATGGACATAGAGGATGAAAGAGCTGGCCGGAGCGAGCTGTGTTAATGTGGCTGGGGTGATGGGCTTCGAGTCTCGGAATGGCGGAATTAGAATCCGGTGCCTTACCGTTTGGCGATACCCCAACTGGGt                                                                                                  <  2:191786/138‑1 (MQ=255)                                                ggcattaaCGCAGAAAAGCAAAAAGCTCGCCGAAGTGAGCTTGTTTAATGTGGCTGGGGTACGAGGATTCGACCCTCGGAATGCCGGAATTAGACTCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg                                                                                       <  1:1228211/137‑1 (MQ=21)                                                  aatgaaCGCAGAAAAGCAAAGAGGTCGCCGGAGCGAGCTTTTTTAATGTGGCTGGGGTGCGGGGGTTCGAGCCTCGGAATGCCGGAATTAGAATCCGGTGCCTTTGCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGt                                                                                     <  2:324378/143‑1 (MQ=25)                                                      agagCAAAAAAGAAAAAAGCGAGCGGAAGCGAGCTTTTTGAACGTGGCTGGGGTACGAGGATTCGAACATCGGAATGCCAGAATTAGAAGCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGc                                                                                 <  2:889103/140‑1 (MQ=18)                                                         gCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGAGTGGCAGTGCAAGGTAAGCGTc                                                                              >  2:1255814/1‑143 (MQ=21)                                                           aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                            <  1:1229784/143‑1 (MQ=255)                                                                 gCAAAAAGCGCGGCGAAGCGAGCGTTTTTAAGGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATaa                                                                      <  2:94040/143‑1 (MQ=21)                                                                  cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTCAGCGTCTTGGATaaa                                                                     >  1:370732/1‑143 (MQ=255)                                                                    aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAtt                                                                   <  1:277137/143‑1 (MQ=255)                                                                     aaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTg                                                                  <  1:882399/143‑1 (MQ=255)                                                                          tCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTgg                                                             >  1:278170/1‑143 (MQ=255)                                                                            gCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGCATTAGCATCCGGTGCCTTACCGCTTGGCGATCCCCCAACTGGGTGCACTTACAAGGTACGCGTCTTGAATACATTGGCTgggg                                                           >  1:900094/1‑143 (MQ=25)                                                                             ccGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACGTACAAGGTAGGCGTCGCGAATACATTGGCTGGGGt                                                          >  2:425737/1‑143 (MQ=21)                                                                                 aGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGa                                                      >  1:257059/1‑143 (MQ=255)                                                                                      gCTTTTTTAATGTGGCTGGGGGACGAGGCTCAGCACCGCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAtt                                                 <  2:556611/143‑1 (MQ=37)                                                                                       cTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTTGCTGGGGTACGAGGATTc                                                >  1:410310/1‑143 (MQ=255)                                                                                           tttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAAc                                            <  1:462642/143‑1 (MQ=255)                                                                                                  tgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGaa                                     >  1:675749/1‑143 (MQ=255)                                                                                                      tGGGGTACGTGGCTTCCAACCTCGGAACGCCGGAATTCGACCCCGTTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTTGAACCTCGGAATGcc                                 <  2:662996/143‑1 (MQ=37)                                                                                                      tGGGGTACGAGGATGCGAACCGCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGcc                                 <  1:1181280/143‑1 (MQ=255)                                                                                                       ggggTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCg                                <  1:261957/143‑1 (MQ=255)                                                                                                         ggTACGAGGATTCGAGCCTGGGAATGCCGGAATTAGACTCCGGTGCATTACCGCTTGGCGATACCCCGACTGGGTGCACGTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGa                              <  2:352932/143‑1 (MQ=38)                                                                                                           aaCGAGGATTCGACCCCCGAAATGCAGGCCTTCGAACCCGCTGTCTCACCGCTTGGCGCTACCCCAACTGGGAGCACGTACAAGGTAGGCGGCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAAt                            <  2:370732/142‑1 (MQ=21)                                                                                                             cGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGGTTCGAACCTCGGAATGCCGGAATCa                          <  1:425737/143‑1 (MQ=255)                                                                                                              gAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAg                         <  1:1127294/143‑1 (MQ=255)                                                                                                                   ttCGAACCTCGGAATGACGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                    <  1:902148/143‑1 (MQ=255)                                                                                                                    tCGAACCTCGGAACGCCGGAAGTAGAGCCCGGTGCCCTCCCGCGTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCg                   <  1:738353/143‑1 (MQ=37)                                                                                                                           tCGGAATGCCGGAATTAGAATTCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTTCAAGGTAAGCGTGTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt            <  1:1079276/143‑1 (MQ=37)                                                                                                                                    cGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGCATCCGGTGCCTTACCGCTTgg   >  1:707557/1‑143 (MQ=255)                                                                                                                                    cGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGACTCCGGTGCCTTACCGCTTgg   >  1:148313/1‑143 (MQ=255)                                                                                                                                    cGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg   >  1:1026426/1‑143 (MQ=255)                                                                                                                                     ggAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGTAATCAAAATCCGGTGCATTACCGCTTCGc  >  2:53883/1‑143 (MQ=37)                                                                                                                                          |                                                                                                                                          CGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGC  >  NC_000913/696333‑696607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑