| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 759,250 | G→T | R182L (CGC→CTC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 759,250 | 0 | G | T | 98.0% | 138.3 / NA | 50 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (27/21); total (29/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG > NC_000913/759110‑759389 | ccagcggaCTTTCTACGTCGTTTCACTGGGTGGCGGCAAAGAAACCATGAATCTCGGCGAGCTGCTGGAAGCCCGCAAGCAATCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAACActct < 2:357758/137‑1 (MQ=255)ccAGGAGCCCTTCGACGTCGGTTCAGTTGCCAGCGGCAAAGAAACCATGAATCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct < 2:1055592/143‑1 (MQ=255) cgacagcccTTCGACTTCGTTTCATTGTCCATCGGCAAAGAAACCATGACACTCGGCGAGCTCCTGGAAGCCATCAATCAAACCTACCGCTGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAATACTCTg < 2:173072/136‑1 (MQ=255) ggggaCCTTCAACGTCTGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGa < 1:933721/140‑1 (MQ=255) ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACa > 1:245007/1‑143 (MQ=255) aCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGATCTGCTGGGAGCCCTCAAGCAAACATGCTGGGGCCCGATTGGGGCCGAGTATAGGAGCATTAGCAGCACCGGAGAAAATCTCTGGATCCTGCAGCga > 2:6590/1‑142 (MQ=255) tCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTc < 1:9814/143‑1 (MQ=255) aGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑Tc‑gcgc < 1:17692/143‑1 (MQ=255) aGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAACCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGACGCAAAACTCTGGATCCAACAGCGTCCCGCCTCTAG‑Tc‑gcgc > 2:232010/1‑143 (MQ=255) cGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGa < 1:1026363/143‑1 (MQ=255) ggCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTGAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGAc < 2:1000091/143‑1 (MQ=255) aaGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTCGATCCAACAGCGTCTCGAGTCTGG‑TC‑GCGCGCCttac > 2:861392/1‑141 (MQ=255) gAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAACCTCTGGAACCAACAGCGTCTCGAGCCTGG‑TC‑GCGCGACTCTCaa > 2:484633/1‑143 (MQ=255) ccATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGc < 1:625319/143‑1 (MQ=255) tCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTGG‑AC‑GCGCGACTTGCAATAGCGAAGAGaaa > 2:1161968/1‑143 (MQ=255) gctaGCTGCTGCAAGCCGTCAAGCAACGCTCCTGCGGCCGGATGGGTGCCGAATATAGGCACATTACGCGCACCGACGAAAAACTCTGAATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGaaaaaa < 2:1185083/140‑1 (MQ=255) cgggctgctGGAAGCCCCCGCGCCAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAAc < 2:822802/140‑1 (MQ=255) ctgctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGAGTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGctt < 1:196508/143‑1 (MQ=255) ctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGcttctt > 1:664118/1‑143 (MQ=255) cgggAAGCACTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGCGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGcttctt < 1:575066/141‑1 (MQ=255) ggAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTaa < 1:1032202/143‑1 (MQ=255) gAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCCCCTTACTAGCGCCGAAGCCACACGCCGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTGCAATAGCGAAGAGACAAAACGCTTCGTAAg > 2:1235252/1‑143 (MQ=255) gAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAGACTCTGGATCCAACAGCGTATCGAGTCTGG‑TG‑GCGCGACTCTCAATAGCGAAGAGAAAAAACGCTTCGTAAg > 1:224174/1‑143 (MQ=255) ccTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGCGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TA‑GCGCGACTTTCAATAGAGACGAGAAAAAACGCTTCTGAAGCAAAc > 2:823274/1‑143 (MQ=255) ccTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAAc > 1:907221/1‑143 (MQ=255) cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACt > 1:29386/1‑143 (MQ=255) gCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTGAAGCGAACTGAccg > 2:660208/1‑143 (MQ=255) cTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg < 1:563443/143‑1 (MQ=255) tGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGCGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAgg > 2:186915/1‑143 (MQ=255) gCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTACTTACGCGAACTGACAGCCGCTGAAGGt > 2:536836/1‑143 (MQ=255) cGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑GC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTc > 1:129424/1‑143 (MQ=255) cctaTTTGTGCCGAGTCTATGCACGGTACCAGCACCGAAGAAAAACTCTGGATCTAACAGCGTATCGAGTCTGG‑CC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGTCCGCCGCTGAAGGTCTTGa < 2:62461/140‑1 (MQ=255) cGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TA‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTCCTTAAGCGAACTGACCGCCGCTGAAGGTCTTgca > 2:1135573/1‑141 (MQ=255) aTTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGATGACGGTCATGAACg > 1:463641/1‑143 (MQ=255) tGCCGAGTATATGCACATTACCAGCATCGAAGAAATTCTTTTGATCTAACATTGTATCGAGTCTGG‑TC‑GCGCGAGTTTCAAGAGCGAAGGGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTAcc < 1:781892/143‑1 (MQ=255) tGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑GC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGCACTGACCGCCCCCGCAGGTCTTGCACGTTAcc > 2:462645/1‑143 (MQ=255) ggggTATAGGCAGATTACGAGCACCGAAGAAAAGGTGTGGAGCCAACAGGGTGTAGAGTCGTG‑TC‑GCGCGACGTTAAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGACGCTGAAGGTCTTGAACGTTACCTCg < 2:300630/140‑1 (MQ=255) cGAGTCTATCCACCTTACCTCCACCGAAGAAAACCTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATCGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAATGTCTTGAACGTTACCTCg < 2:1244986/143‑1 (MQ=255) gAGTATATGCACATTACCAGCACCGAAGAAAAACTAAGGATCCAACAGCGTACCAAGTCTGG‑TC‑GAACGACGAGCAATAGCGTAGAGGCAAAACGCTACTACAGCGAAGTGACCACGGCTGGAGGTCTTAAACGTTATCTCgg > 2:1028374/1‑143 (MQ=255) aGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGCCTTTCAATAGCGAAGAGAAAAAACGCTTCTTCAGCGAACTGACCGCCCCTGAAGGTCTTGCACGTTCACTCGgc > 2:737686/1‑143 (MQ=255) aGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgc < 1:1245275/143‑1 (MQ=255) catatGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGAACAGAGCGAC‑‑CGGAGAGGGGAGAGAGCAAACCCCTCTTAACCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgcgc < 1:294770/142‑1 (MQ=255) gCACATTACCAGCACCGAAGAAAAACCATGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGCCTTGAACGATACCTCGGAGCAACAt > 2:1110388/1‑143 (MQ=255) gCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGACAAAACGCTTCTTAAGCGAACTGACCGCCGCCGAAGGTCGTGAACGTTACCTCGGCGAAAAACTCCCTGgcgcc > 2:1246390/1‑142 (MQ=255) cACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg < 2:4243/104‑1 (MQ=255) cACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg > 1:4243/1‑104 (MQ=255) ccGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCaaaa > 2:368547/1‑143 (MQ=255) cGAAGAAAAACTCTGGATCCAACAGCGTAACGAGTGTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAAc > 1:872516/1‑143 (MQ=255) gaagaaAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGAGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAACTCCCTGGCGCAAAACg > 2:944234/1‑143 (MQ=255) gaaAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCtt > 1:315867/1‑143 (MQ=255) aaaaCTCTGGATCCAACAGCGTATCGAGTCTGG‑TT‑GATCTACGTGCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTtct < 1:859955/143‑1 (MQ=255) aaCTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCCTGAACGTTACCTCGGCGCCAAATTCCCTGGGGCACAACGCTTCCCg > 2:725871/1‑143 (MQ=255) | CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG > NC_000913/759110‑759389 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |