Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative colanic acid biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	161.4 / NA	53	I204S (ATC→AGC)	wcaA	putative colanic acid biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/34);  total (19/34)

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132650‑2132925                                                                                                                                          |                                                                                                                                           gcggggagagGGTGAACAGCCGAAATGTGTAGCGGGCGCGGACGAATGTGTCTGGGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                       <  1:219783/137‑1 (MQ=255)   tGGTAGAGGGTAAACAGCTGTGATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGt                                                                                                                                     <  1:971006/143‑1 (MQ=255)   tGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTGTGTGCTGGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGt                                                                                                                                     <  1:143998/143‑1 (MQ=255)     gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCTCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                   >  2:453239/1‑143 (MQ=255)      gatagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                  <  1:460764/140‑1 (MQ=255)      gagagGGTAAACGGCTGATATTTTTTGCCGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                  <  2:76561/142‑1 (MQ=255)         agGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctc                                                                                                                               <  1:454904/143‑1 (MQ=255)           ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt                                                                                                                             <  1:11220/143‑1 (MQ=255)           ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt                                                                                                                             >  1:885146/1‑143 (MQ=255)              aaaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTACGGTGAAAGTGGAAGTACCCGGAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATCGCTATGCAGGCTCGGCGTCGCCTCTGCTa                                                                                                                          >  1:159840/1‑143 (MQ=255)                      gATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCAc                                                                                                                  <  1:813343/143‑1 (MQ=255)                       atatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAGGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                  >  2:674407/1‑126 (MQ=255)                       atatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAGGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                  <  1:674407/126‑1 (MQ=255)                       atatTTTTTGCTGGCGCGGTCGAATTTGTCTGTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGGTGGATATGCAGGCTCTGCGTCGCCTCTTCTCCTTTCcgcg                                                                                                                 >  1:763230/1‑143 (MQ=255)                         atTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTGGGTGACGAGGTGATCTGCACTTCACCTTGATTGATATGCAGGCTCTGCGTCGCCTGCTGTGCTTTCCACGGt                                                                                                               >  2:380928/1‑143 (MQ=255)                                     cgcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGCAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTGTCCAAGGTTCGCCGTACTcc                                                                                                   >  2:1128365/1‑143 (MQ=255)                                         gTCGATTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacca                                                                                               <  2:985535/143‑1 (MQ=255)                                         gTCGACTTTGTCTTTGTCCTTGCGGTAAAGGTGGAAGTACGCGGAGAATTTTTTCGGGGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTGTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacca                                                                                               <  2:323315/143‑1 (MQ=255)                                         gTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacca                                                                                               <  1:210999/143‑1 (MQ=255)                                         gTCGAATTTGTCCTTGTGCTGGCGGTAAAAGTGGAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacca                                                                                               <  1:659161/143‑1 (MQ=255)                                             aaTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCAACGGTTCGCCGTACTCCACCACCAt                                                                                           >  1:703118/1‑143 (MQ=255)                                                  gTCGTTGTGCTCGCGGTACTAGTGGAAGTACCCGGTGAATTTTTTCGGTGACGAGGTGATCTGCATTCCACCGTGATTGATAGGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCa                                                                                      <  2:167792/143‑1 (MQ=255)                                                   tcgttGTGCATGCGGTAAAAGTGGAAGTACCCGGAGAATTGTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATTTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAg                                                                                     <  1:179368/140‑1 (MQ=255)                                                     tttGTGCTTGCGGTAAAATTGGAAGTACCCGAAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGCGATTGATATGCAGGCTCTGCATCGCCTCTTCTACTTTCCACCGTCCGCCGTACTCCACGACCCTcctctgca                                                                                   >  2:454116/1‑137 (MQ=255)                                                      ttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                                  <  1:988594/143‑1 (MQ=255)                                                        gtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGa                                                                                <  1:453239/143‑1 (MQ=255)                                                         tgcgtgcgGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCCACCTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGAt                                                                               <  1:353464/143‑1 (MQ=255)                                                             tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCg                                                                           <  1:827023/143‑1 (MQ=255)                                                                ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGCACTCCACCACCATCGGCAGAAAGAtgttgtga                                                                        >  1:1191597/1‑141 (MQ=255)                                                                 ggaaaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAt                                                                       <  2:401150/141‑1 (MQ=255)                                                                 gTAAAAGTGGAAGTACCAGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt                                                                                                                       <  1:850571/95‑1 (MQ=255)                                                                 gTAAAAGTGGAAGTACCAGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt                                                                                                                       >  2:850571/1‑95 (MQ=255)                                                                  tcaaaCTGAAACTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTCCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc                                                                      <  1:543716/141‑1 (MQ=255)                                                                      aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGCTCGCCGTCCTCCACCACCCCCCGCCGGCCGCTGTCGTACTCCtgt                                                                  >  1:611048/1‑143 (MQ=255)                                                                          gaattgcccGGACAATTTTTTCGGTGGCGAGGTGATCTGCATTCCTACGTGATTGAGATGCAGGCTCTGCGTCGCCTCTTCTACTTGTCTAGGTGCGCCGGACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCg                                                              <  2:829598/137‑1 (MQ=255)                                                                          gAAGCATCCGGAGAGTTTGTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCg                                                              <  2:946487/143‑1 (MQ=255)                                                                           acgTACCCGGAGAATCTTTTCCGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGc                                                             <  2:1068772/141‑1 (MQ=255)                                                                                cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                        <  2:885146/143‑1 (MQ=255)                                                                                 ccGGAGAATTTTTTCGGTGACGAGGTGAGCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAg                                                       <  1:1192636/143‑1 (MQ=255)                                                                                   tggggtttttttCGGTGACGAGGTGAGCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                     <  2:159840/137‑1 (MQ=255)                                                                                              ccggggccGAGGTTATCTGCTGTGCTCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGGACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaac                                          <  1:517686/136‑1 (MQ=255)                                                                                                ggTGACGAGGTGATCTGCATTTCACCGTGATTTATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaa                                        <  1:571248/143‑1 (MQ=255)                                                                                                  tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGACGATGTCGTAACCCAGCACCTTTTTCTGTTCGGTATCGaacaaac                                      >  1:1128393/1‑143 (MQ=255)                                                                                                   gACGGGGTGGTCTGCATTTCACCGTGATTGATCTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCGCCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                     <  2:795496/143‑1 (MQ=255)                                                                                                           gATCTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa                             <  2:726677/143‑1 (MQ=255)                                                                                                                gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTCATCCTGTGCCGCTTACACCTCGGTATCGAACAAACCCTCCTTGaaccca                        >  2:494345/1‑139 (MQ=255)                                                                                                                         cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg               <  1:971509/143‑1 (MQ=255)                                                                                                                             attgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGATGTCGTAATCCTGTTCCGCTTTCAGGTCGGTATCGAACAAACACTATTTGACACGCCATCCACAgctca           >  2:57372/1‑139 (MQ=255)                                                                                                                             attgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaa           >  2:676473/1‑143 (MQ=255)                                                                                                                              ttgagATGCAGCCTCTGCGTCGCCCCTTCTACTTTCCACGGTTCGCCGCACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa          <  2:1017804/143‑1 (MQ=255)                                                                                                                                   atGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCATTGAAACGCCATGCCCAGGTAAAGACCt     >  2:990229/1‑143 (MQ=255)                                                                                                                                     gCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCCCCACCATCCGCAGGAGGGTGTCGTAATCCTGTGCCGCTTTCAGTGCGGTATCGACCAACCACTCTTTGAAACGCCATGCCCAGGGGAAGACCTgg   >  1:1245117/1‑143 (MQ=255)                                                                                                                                      cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCACAGCTAAAGACCAGGt  >  2:752914/1‑143 (MQ=255)                                                                                                                                          |                                                                                                                                           TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132650‑2132925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑