| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,168,475 | 2 bp→TG | intergenic (+28/‑165) | gltT → / → rrlB | tRNA‑Glu/23S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,168,475 | 0 | G | T | 92.3% | 17.3 / NA | 13 | intergenic (+28/‑166) | gltT/rrlB | tRNA‑Glu/23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (3/9); total (4/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 4,168,476 | 0 | A | G | 100.0% | 18.8 / NA | 13 | intergenic (+29/‑165) | gltT/rrlB | tRNA‑Glu/23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (4/9); total (4/9) | |||||||||||
TATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCG > NC_000913/4168341‑4168502 || tatCGCGCAACGCGTGATAAGCAATGTTCGTGGCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGGGAGTGAAAGTCACCTGCCTTAat < 2:237275/143‑1 (MQ=11)gatCACGCAACGCGTGATAAGCAATTTTCGTGTCCCATTCGTGTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAat < 1:405097/142‑1 (MQ=11) tcgcggaaCGCGGGACAAGCAATTTTCGTGGCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGGAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAatat < 2:792366/137‑1 (MQ=11) tcccGCAACGCGTGATAAGCAATTCTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAatat < 2:633804/140‑1 (MQ=11) aCGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATAtct < 1:626845/143‑1 (MQ=255) aCGCAACGCGTGATAAGCAATTTTCGTGCCCCCTGCGTCTAGAGGCCCAGGACACCGCCCTTTCCCGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATAtct < 2:589415/143‑1 (MQ=11) cGCAACGCGTGATAAGCAATTGTCGTGTCCCCTTCGTCTAGCGGACCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATAtctc < 1:347342/143‑1 (MQ=11) gCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGCCACCGCCCTTTCACGGCGGTACCAGGGTTTCGAATCCCCTAGGGGACGCAAATTGCTGATTAGTGAGTGAAAGTAAACTGCCTTAATATCTCa > 1:185252/1‑143 (MQ=11) gCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCa < 1:944846/143‑1 (MQ=255) aaCGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCaaa > 1:763574/1‑143 (MQ=12) tAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACAAAGCATTATCTCGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCtt < 1:1200831/143‑1 (MQ=11) aGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCAGCTTCg > 1:673035/1‑143 (MQ=255) aGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCGGCCTTAATATCTCAAAACTCATCTTCg > 2:58842/1‑143 (MQ=255) || TATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCG > NC_000913/4168341‑4168502 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |