BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,393	G→T	W27C (TGG→TGT)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,393	0	G	T	98.9%	1023.5 / ‑1.2	276	W27C (TGG→TGT)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand): ref base G (1/2); new base T (125/148); total (126/150)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

CTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTC  >  NC_000913/3041299‑3041489

cTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCt                                                                                             >  1:5395571/1‑100 (MQ=255)

cTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCt                                                                                             >  1:6369007/1‑100 (MQ=255)

    ggACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGcc                                                                                         >  1:4016602/1‑100 (MQ=255)

    ggACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGcc                                                                                         <  1:2434775/100‑1 (MQ=255)

       ctctATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGccacc                                                                                      >  1:3921811/1‑100 (MQ=255)

       ctctATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGccacc                                                                                      >  1:2495394/1‑100 (MQ=255)

         ctATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTgcg                                                                                               <  2:3467258/89‑1 (MQ=255)

          tattatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTgcgc                                                                                              <  2:6007779/89‑1 (MQ=255)

          tattatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCAtt                                                                                   <  1:5706754/100‑1 (MQ=255)

            ttatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTAc                                                                                 <  1:3446542/100‑1 (MQ=255)

             tatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTg                                                                                           >  2:1685126/1‑89 (MQ=255)

              atGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGc                                                                                          >  2:2097314/1‑89 (MQ=255)

              atGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTg                                                                               <  1:3825369/100‑1 (MQ=255)

              atGGCTTTTACACCGTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTg                                                                               <  1:3776491/100‑1 (MQ=255)

               tGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTgg                                                                              <  1:1033128/100‑1 (MQ=255)

                 gCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGgcg                                                                            >  1:1111403/1‑100 (MQ=255)

                   ttttACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGcgcg                                                                          <  1:1987852/100‑1 (MQ=255)

                    tttACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCgg                                                                         <  1:4971823/100‑1 (MQ=255)

                        cacCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAg                                                                     >  1:670910/1‑100 (MQ=255)

                          ccTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCg                                                                   <  1:5058281/100‑1 (MQ=255)

                          ccTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGAAGCTTGATGACTGTGCGCTTGCCACCATTACTgg                                                                              <  2:4901358/89‑1 (MQ=255)

                           cTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGgc                                                                             >  2:2447838/1‑89 (MQ=255)

                           cTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGa                                                                  <  1:2063674/100‑1 (MQ=255)

                           cTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGa                                                                  >  1:1338539/1‑100 (MQ=255)

                              ttCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGcgcg                                                                          >  2:2735051/1‑89 (MQ=255)

                              ttCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGaaaa                                                               <  1:622272/100‑1 (MQ=255)

                                cctccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAat                                                             >  1:53225/1‑100 (MQ=255)

                                cctccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAat                                                             <  1:6018693/100‑1 (MQ=255)

                                cctccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAat                                                             <  1:4257310/100‑1 (MQ=255)

                                 ctccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGAc                                                                       >  2:3527353/1‑89 (MQ=255)

                                 ctccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAata                                                            >  1:819126/1‑100 (MQ=255)

                                 ctccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAata                                                            >  1:2888535/1‑100 (MQ=255)

                                  tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAatat                                                           <  1:1736677/100‑1 (MQ=255)

                                  tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAatat                                                           <  1:1910596/100‑1 (MQ=255)

                                   ccccGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAatata                                                          <  1:3250406/100‑1 (MQ=255)

                                      cGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGa                                                                  <  2:3372520/89‑1 (MQ=255)

                                      cGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGc                                                       <  1:1492070/100‑1 (MQ=255)

                                        tCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAg                                                     <  1:3111196/100‑1 (MQ=255)

                                        tCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAg                                                     <  1:1632512/100‑1 (MQ=255)

                                           gCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAat                                                             >  2:4928589/1‑89 (MQ=255)

                                            ccTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTc                                                 >  1:3000478/1‑100 (MQ=255)

                                             cTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCa                                                >  1:5604707/1‑100 (MQ=255)

                                              tACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCAGAAAAAGAAAAatata                                                          <  2:4033942/89‑1 (MQ=255)

                                               aCGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAgg                                              <  1:4769128/100‑1 (MQ=255)

                                                cGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATg                                                        >  2:5432783/1‑89 (MQ=255)

                                                cGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGt                                             <  1:2580719/100‑1 (MQ=255)

                                                cGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCAAAAAATATATg                                                        <  2:3203289/89‑1 (MQ=255)

                                                 ggCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTg                                            <  1:4503224/100‑1 (MQ=255)

                                                  gCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCa                                                      <  2:154352/89‑1 (MQ=255)

                                                    ttCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAca                                         <  1:4436241/100‑1 (MQ=255)

                                                      cTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGt                                                  >  2:3763475/1‑89 (MQ=255)

                                                       tGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTc                                                 >  2:2960605/1‑89 (MQ=255)

                                                       tGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagca                                      <  1:3519979/100‑1 (MQ=255)

                                                       tGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagca                                      <  1:2622986/100‑1 (MQ=255)

                                                       tGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagca                                      <  1:1458596/100‑1 (MQ=255)

                                                        gCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCa                                                >  2:1033561/1‑89 (MQ=255)

                                                        gCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagcag                                     >  1:466722/1‑100 (MQ=255)

                                                         cccGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAg                                               >  2:3302856/1‑89 (MQ=255)

                                                         cccGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAg                                               >  2:3570841/1‑89 (MQ=255)

                                                         cccGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGa                                    <  1:2311053/100‑1 (MQ=255)

                                                         cccGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGCCAg                                               >  2:5346114/1‑89 (MQ=255)

                                                          ccGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGAt                                   >  1:5997982/1‑100 (MQ=255)

                                                           cGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGt                                             <  2:5737658/89‑1 (MQ=255)

                                                            gTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGAAAAAAAAAAAAAATATATGTAGGGTCAGGTg                                            <  2:3941979/89‑1 (MQ=255)

                                                            gTTTACCGCTGACGCTGATGACGCTCGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTg                                            <  2:3274810/89‑1 (MQ=255)

                                                               tACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAg                              >  1:860573/1‑100 (MQ=255)

                                                               tACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAg                              >  1:6314886/1‑100 (MQ=255)

                                                               tACCGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGAGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAg                              <  1:6398336/100‑1 (MQ=255)

                                                                 ccGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagc                                       >  2:4320150/1‑89 (MQ=255)

                                                                 ccGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGcc                            >  1:4370877/1‑100 (MQ=255)

                                                                 ccGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGcc                            <  1:4839046/100‑1 (MQ=255)

                                                                 ccGCTGACGCTGATGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGcc                            <  1:4916928/100‑1 (MQ=255)

                                                                   gctgacgctgaTGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagcag                                     >  2:5264537/1‑89 (MQ=255)

                                                                   gctgacgctgaTGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAg                          >  1:5891550/1‑100 (MQ=255)

                                                                     tgacgctgaTGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGAt                                   >  2:1112313/1‑89 (MQ=255)

                                                                      gacgctgaTGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATg                                  <  2:5229383/89‑1 (MQ=255)

                                                                      gacgctgaTGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATg                                  >  2:4624481/1‑89 (MQ=255)

                                                                      gacgctgaTGACGCTTGAAGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATg                                  <  2:1841807/89‑1 (MQ=255)

                                                                        cgctgaTGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTc                                <  2:1000456/89‑1 (MQ=255)

                                                                        cgctgaTGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGc                     >  1:358863/1‑100 (MQ=255)

                                                                          ctgaTGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAg                              <  2:2634192/89‑1 (MQ=255)

                                                                          ctgaTGACGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCag                   <  1:4782492/100‑1 (MQ=255)

                                                                           tgatgaCGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGc                             >  2:1138824/1‑89 (MQ=255)

                                                                            gatgaCGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGcc                            <  2:2255513/89‑1 (MQ=255)

                                                                            gatgaCGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaa                 >  1:2158335/1‑100 (MQ=255)

                                                                            gatgaCGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaa                 >  1:3060854/1‑100 (MQ=255)

                                                                              tgaCGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAg                          >  2:4799232/1‑89 (MQ=255)

                                                                              tgaCGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaaga               <  1:4153916/100‑1 (MQ=255)

                                                                                 cGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATg                       <  2:2070502/89‑1 (MQ=255)

                                                                                 cGCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATg                       <  2:6336634/89‑1 (MQ=255)

                                                                                  gCTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATgg                      <  2:6110735/89‑1 (MQ=255)

                                                                                   cTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGc                     <  2:670910/89‑1 (MQ=255)

                                                                                   cTTGATGACTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGc          <  1:1671371/100‑1 (MQ=255)

                                                                                     tgatgaCTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCAc        >  1:5478778/1‑100 (MQ=255)

                                                                                       atgaCTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCt      >  1:2737215/1‑100 (MQ=255)

                                                                                        tgaCTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaag                <  2:4250885/89‑1 (MQ=255)

                                                                                        tgaCTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaag                <  2:2799861/89‑1 (MQ=255)

                                                                                        tgaCTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTg     >  1:1977955/1‑100 (MQ=255)

                                                                                           cTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATc             >  2:781720/1‑89 (MQ=255)

                                                                                           cTGTGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTc  >  1:5270293/1‑100 (MQ=255)

CTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTC  >  NC_000913/3041299‑3041489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`