BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,950,965:1	+C	8.0%	coding (134/567 nt)	yecD →	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,950,965	1	.	C	8.0%	486.8 / 39.9	125	coding (134/567 nt)	yecD	putative hydrolase
Reads supporting (aligned to +/- strand): ref base . (55/60); new base C (7/3); total (62/63)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

TTTTTCAGGAGTCATTATGCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGAC  >  NC_000913/1950816‑1951113

tttttCAGGAGTCATTATGCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTcg                                                                                                                                                       <  1:449736/150‑1 (MQ=255)

    tCAGGAGTCATTATGCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGC‑GCGAAATTTcg‑ccgcc                                                                                                                                                 <  2:796048/150‑5 (MQ=255)

    tCAGGAGTCATTATGCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGC‑GCGAAATTTcg‑ccgcc                                                                                                                                                 <  2:804430/150‑5 (MQ=255)

      aGGAGTCATTATGCTTGAACTTAATGCTAAAACCACTGCGCTGGGGGTGATCGATTTACAACACGGCATCTTACCTTTTGCCGGGGGGCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGC‑GCGAAATTTCGCCGCCAg                                                                                                                                                <  1:1109932/150‑1 (MQ=255)

       tgAGTCATTATGCTTTAACTTAATGCTAAAACCACTGATCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTTCCTGTTGTCAACTTACTGCCGATGAGGTAGTTAATCTCGCCGGTAAGCTGGC‑GAGAAATTTCGCCGCCAGc                                                                                                                                               <  2:1649927/149‑1 (MQ=255)

       ggAGTCATTATGCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGC‑GCGAAATTTCGCCGCCAGc                                                                                                                                               <  2:112086/150‑1 (MQ=255)

         aGTCATTATGCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCgg                                                                                                                                             >  1:1470223/1‑150 (MQ=255)

            cATTATGCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCa                                                                                                                                          >  1:1881189/1‑150 (MQ=255)

            cATTATGCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTAACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCa                                                                                                                                          >  1:1267512/1‑150 (MQ=255)

                 tccTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCg                                                                                                                                     >  1:440912/3‑150 (MQ=255)

                  gCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCgt                                                                                                                                    <  1:134213/150‑1 (MQ=255)

                  gCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCgt                                                                                                                                    >  1:1587992/1‑150 (MQ=255)

                  gCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCgt                                                                                                                                    >  1:996952/1‑150 (MQ=255)

                   cTTGCACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGACGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCgtg                                                                                                                                   <  1:2306768/150‑1 (MQ=255)

                     tGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCACGTGtt                                                                                                                                 >  1:1586056/1‑150 (MQ=255)

                       aaCTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTc                                                                                                                               <  2:1054252/150‑1 (MQ=255)

                         cTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAAATTCG‑CGCCAGCGGTCAGCCCGTGTTTCTg                                                                                                                             >  2:2162468/1‑150 (MQ=255)

                          ttAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTgg                                                                                                                            <  2:74689/150‑1 (MQ=255)

                           tAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGt                                                                                                                           <  1:1663926/150‑1 (MQ=255)

                           tAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGt                                                                                                                           >  2:1613996/1‑150 (MQ=255)

                              tGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTgcg                                                                                                                        <  1:543928/150‑1 (MQ=255)

                              tGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTgcg                                                                                                                        <  2:1754406/150‑1 (MQ=255)

                                cTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGcgcg                                                                                                                      >  1:1235310/1‑150 (MQ=255)

                                  aaaaCCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGtt                                                                                                                    <  1:531723/150‑1 (MQ=255)

                                     aCCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTATTTAATCGCGCCGGGAAGCTGGCTTCTAAATTTCG‑CGCCATCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGc                                                                                                                 <  2:1124465/150‑1 (MQ=255)

                                     aCCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGc                                                                                                                 <  1:214285/150‑1 (MQ=255)

                                      ccACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCt                                                                                                                >  1:83539/1‑150 (MQ=255)

                                       cACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACGTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGATGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTg                                                                                                               <  2:1655547/150‑1 (MQ=255)

                                         cTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGt                                                                                                             <  2:363706/150‑1 (MQ=255)

                                          tGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTc                                                                                                            <  2:63119/150‑1 (MQ=255)

                                             gcTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGc                                                                                                         >  1:292560/1‑150 (MQ=255)

                                               tggtggTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCg                                                                                                       >  1:139831/1‑150 (MQ=255)

                                                   ggtgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTa                                                                                                   >  2:1532433/1‑150 (MQ=255)

                                                   ggtgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTa                                                                                                   >  2:943392/1‑150 (MQ=255)

                                                     tgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACg                                                                                                 >  2:469492/1‑150 (MQ=255)

                                                     tgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACg                                                                                                 >  2:1572297/1‑150 (MQ=255)

                                                      gATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGc                                                                                                <  2:1243248/150‑1 (MQ=255)

                                                           aTTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAg                                                                                           <  1:611056/150‑1 (MQ=255)

                                                           aTTTACAAGAAGCCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTAGGCTGGTCTGCCGATTACGCTGAAg                                                                                           >  2:1959499/1‑150 (MQ=255)

                                                            tttACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGc                                                                                          >  1:827619/1‑150 (MQ=255)

                                                             ttACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCa                                                                                         <  2:1881189/150‑1 (MQ=255)

                                                              tACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACAGCCGATGAGGTAGTTAATAGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGAGTTGGCTGGTCTGCCGATTACGCTGAAGCAt                                                                                        >  1:1804116/1‑150 (MQ=255)

                                                              tACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACAGACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCAt                                                                                        >  1:106700/1‑150 (MQ=255)

                                                               aCAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCAtt                                                                                       <  2:2445909/150‑1 (MQ=255)

                                                                cAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTa                                                                                      <  1:2169115/150‑1 (MQ=255)

                                                                  agaagGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTaaa                                                                                    <  2:2081298/150‑1 (MQ=255)

                                                                    aagGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAAc                                                                                  >  1:58811/1‑150 (MQ=255)

                                                                     agGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACa                                                                                 <  1:1039915/150‑1 (MQ=255)

                                                                          tCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCgg                                                                            <  2:199590/150‑1 (MQ=255)

                                                                           cTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGt                                                                           <  2:1908977/150‑1 (MQ=255)

                                                                            ttACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTc                                                                          >  2:956015/1‑150 (MQ=255)

                                                                             tACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCg                                                                         <  1:1895009/150‑1 (MQ=255)

                                                                             tACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCg                                                                         <  1:1885869/150‑1 (MQ=255)

                                                                                  tttGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCGTGTGTTTTTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGTTCGATgcg                                                                    <  2:1267512/150‑1 (MQ=255)

                                                                                    tGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGcc                                                                  <  2:586677/150‑1 (MQ=255)

                                                                                     gCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGccc                                                                 <  1:469492/150‑1 (MQ=255)

                                                                                       cGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTc                                                               <  1:1572297/150‑1 (MQ=255)

                                                                                           ggtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCg                                                           <  2:292560/150‑1 (MQ=255)

                                                                                           ggtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCg                                                           >  2:1322293/1‑150 (MQ=255)

                                                                                            gtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCgg                                                          <  1:2267341/150‑1 (MQ=255)

                                                                                             tCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGc                                                         >  1:2152248/1‑150 (MQ=255)

                                                                                             tCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGc                                                         <  1:1789461/150‑1 (MQ=255)

                                                                                             tCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGc                                                         <  1:584178/150‑1 (MQ=255)

                                                                                             tCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGc                                                         >  2:1794755/1‑150 (MQ=255)

                                                                                                acaTACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTaa                                                      <  2:1235310/150‑1 (MQ=255)

                                                                                                  aTACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAg                                                    <  1:2314775/150‑1 (MQ=255)

                                                                                                   tACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAgt                                                   >  2:437741/1‑150 (MQ=255)

                                                                                                    aCTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAgtg                                                  >  1:2480719/1‑150 (MQ=255)

                                                                                                      tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGtt                                                <  2:2081499/150‑1 (MQ=255)

                                                                                                      tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGtt                                                >  1:2363339/1‑150 (MQ=255)

                                                                                                       gCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTg                                               >  2:335365/1‑150 (MQ=255)

                                                                                                        ccGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGc                                              <  1:169109/150‑1 (MQ=255)

                                                                                                        ccGATGAGGTAGTTAATCGCGCCCGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGc                                              >  2:1875513/1‑150 (MQ=255)

                                                                                                         cGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGcc                                             <  2:1491888/150‑1 (MQ=255)

                                                                                                             gAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGaa                                         <  1:937855/150‑1 (MQ=255)

                                                                                                              aGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGaaa                                        >  1:2415292/1‑150 (MQ=255)

                                                                                                              aGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGaaa                                        >  1:750405/1‑150 (MQ=255)

                                                                                                                   gTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATtgg                                   <  1:2342458/150‑1 (MQ=255)

                                                                                                                    ttAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATtggt                                  >  2:1063269/1‑150 (MQ=255)

                                                                                                                     tAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATtggtg                                 >  2:107732/1‑150 (MQ=255)

                                                                                                                     tAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATtggtg                                 <  2:457755/150‑1 (MQ=255)

                                                                                                                      aaTCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATtggtgg                                <  1:856215/150‑1 (MQ=255)

                                                                                                                       aTCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGc                               >  1:334212/1‑150 (MQ=255)

                                                                                                                         cgcgCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCaa                             <  2:139831/150‑1 (MQ=255)

                                                                                                                            gcCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACAt                          <  1:1052438/150‑1 (MQ=255)

                                                                                                                             ccGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATc                         >  1:2460376/1‑150 (MQ=255)

                                                                                                                              ctgaccgCTGGC‑GCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATcc                        >  2:1488775/7‑150 (MQ=255)

                                                                                                                                gaccgCTGGC‑GCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCctg                      >  2:1388507/5‑150 (MQ=255)

                                                                                                                                 accgCTGGC‑GCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCctgc                     >  1:903392/4‑150 (MQ=255)

                                                                                                                                  ccgCTGGC‑GCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCctgct                    >  2:644230/3‑150 (MQ=255)

                                                                                                                                    gCTGGC‑GCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCtgctgc                  >  1:2371624/1‑150 (MQ=255)

                                                                                                                                    cgaaggcgcgAAATTTCGCCGCCAGCCGTCAGCCAGTGTTTCTGGTGCGCGTTGGCTGGTCTGCAGATTACGCTGAAGCATTAAAACAGCCGGTCGAAGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCctgctg                   >  2:1666420/8‑150 (MQ=255)

                                                                                                                                      tGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGAGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCAt                >  2:1450607/1‑150 (MQ=255)

                                                                                                                                          ggcgAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAgg            >  2:2232624/1‑150 (MQ=255)

                                                                                                                                            cgAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTg          >  2:1507391/1‑150 (MQ=255)

                                                                                                                                            cgAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTg          <  1:1871839/150‑1 (MQ=255)

                                                                                                                                            cgAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTg          <  1:1287354/150‑1 (MQ=255)

                                                                                                                                                aTTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAAc      >  1:1702471/1‑150 (MQ=255)

                                                                                                                                                aTTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAAc      >  2:182949/1‑150 (MQ=255)

                                                                                                                                                    cg‑cgCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGAc  >  2:924708/1‑150 (MQ=255)

TTTTTCAGGAGTCATTATGCTTGAACTTAATGCTAAAACCACTGCGCTGGTGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCG‑CGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGAC  >  NC_000913/1950816‑1951113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`