BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,400,334:1	+T	5.6%	coding (47/309 nt)	hfq →	RNA‑binding protein Hfq

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,400,334	1	.	T	5.6%	800.2 / 41.3	196	coding (47/309 nt)	hfq	RNA‑binding protein Hfq
Reads supporting (aligned to +/- strand): ref base . (98/87); new base T (9/2); total (107/89)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

Only 100 of 200 total aligned reads displayed.

GTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTC  >  NC_000913/4400186‑4400483

gTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑t                                                                                                                                                      <  1:1080601/150‑1 (MQ=255)

 tGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑Tc                                                                                                                                                     >  1:800798/1‑150 (MQ=255)

 tGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑Tc                                                                                                                                                     >  1:1454702/1‑150 (MQ=255)

  gCTATCGCGGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCg                                                                                                                                                    >  2:853977/1‑150 (MQ=255)

   cTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCgg                                                                                                                                                   <  2:946470/150‑1 (MQ=255)

   cTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCgg                                                                                                                                                   <  1:1772177/150‑1 (MQ=255)

     aTCGCAGGCTGAATGTGTACATTTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGGTCAAAGTACAAAGAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGa                                                                                                                                                 >  2:1824518/1‑150 (MQ=255)

       cGCAGGCTGAATGTGAACAAATGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAAc                                                                                                                                               >  2:9215/1‑150 (MQ=255)

        gCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACg                                                                                                                                              >  2:195556/1‑150 (MQ=255)

        gCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACg                                                                                                                                              <  1:1697602/150‑1 (MQ=255)

        gCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACg                                                                                                                                              <  1:1597995/150‑1 (MQ=255)

        gCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACg                                                                                                                                              <  2:1564076/150‑1 (MQ=255)

        gCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCCG‑ACGCAGTGCGTTCAGGAACg                                                                                                                                              <  2:1437037/150‑1 (MQ=255)

           ggCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACgtgt                                                                                                                                           >  1:1737834/1‑150 (MQ=255)

           ggCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACgtgt                                                                                                                                           <  2:44605/150‑1 (MQ=255)

           ggCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCCG‑ACGCAGTGCGTTCAGGAACggat                                                                                                                                           <  2:1426208/150‑4 (MQ=255)

            gCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGtt                                                                                                                                          <  1:416242/150‑1 (MQ=255)

                 aTGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCATGAACGCAATGAG‑TCGGGAACGTGTTCCAGt                                                                                                                                     >  1:2214771/1‑150 (MQ=255)

                    tgtACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTc                                                                                                                                  <  2:2169925/150‑1 (MQ=255)

                    tgtACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTc                                                                                                                                  <  1:2262306/150‑1 (MQ=255)

                    tgtACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTc                                                                                                                                  <  1:2094438/150‑1 (MQ=255)

                     gtACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCt                                                                                                                                 >  2:2336213/1‑150 (MQ=255)

                     gtACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCt                                                                                                                                 >  2:2359527/1‑150 (MQ=255)

                     gtACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCt                                                                                                                                 >  1:412820/1‑150 (MQ=255)

                      tACTATTGAGACGTTTCTTGCGCAATTTTTTAAGAATGGATATGTTAAAAGTACAAATTAGAATATATGGTAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCAGTGCG‑TCGGTAACGGGTTCCAGTTTCta                                                                                                                                <  2:1120551/150‑1 (MQ=255)

                      tACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAACGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCta                                                                                                                                >  2:1918931/1‑150 (MQ=255)

                         aaTTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCtattt                                                                                                                             <  2:1268509/150‑1 (MQ=255)

                          aTTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCtattta                                                                                                                            >  1:1356276/1‑150 (MQ=255)

                           ttGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGCATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCtatttat                                                                                                                           >  2:795343/1‑150 (MQ=255)

                           ttGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCtatttat                                                                                                                           >  1:1986258/1‑150 (MQ=255)

                             gagaCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTatttattt                                                                                                                         <  1:571914/150‑1 (MQ=255)

                              agaCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTg                                                                                                                        <  2:1368239/150‑1 (MQ=255)

                               gaCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTgg                                                                                                                       >  2:2022300/1‑150 (MQ=255)

                               gaCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTgg                                                                                                                       >  2:2453748/1‑150 (MQ=255)

                                 cGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTg                                                                                                                     <  1:1148809/150‑1 (MQ=255)

                                 cGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTg                                                                                                                     <  1:727647/150‑1 (MQ=255)

                                    aTCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAACTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAAt                                                                                                                  <  1:673606/150‑1 (MQ=255)

                                     tCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATg                                                                                                                 <  2:463904/150‑1 (MQ=255)

                                       gTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATATTTACAAGATCCGTTCCTGAAAGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGt                                                                                                               >  1:345299/1‑150 (MQ=255)

                                         gcgcAATTTTATCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTAt                                                                                                             <  2:1768392/150‑1 (MQ=255)

                                          cgcAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTAtt                                                                                                            >  1:1290163/1‑150 (MQ=255)

                                           gcAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTg                                                                                                                        <  2:2230459/137‑1 (MQ=255)

                                            cAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTaa                                                                                                          >  2:2070902/1‑150 (MQ=255)

                                              aTTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGc                                                                                                        >  2:1293365/1‑150 (MQ=255)

                                                tttttCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCAATGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTg                                                                                                      >  1:2177725/1‑150 (MQ=255)

                                                 ttttCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGTGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTaa                                                                                                          <  1:1189401/145‑1 (MQ=255)

                                                 ttttCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTaa                                                                                                          >  2:1189401/1‑145 (MQ=255)

                                                     cAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAgg                                                                                                 <  2:1042734/150‑1 (MQ=255)

                                                         aTCGAAAGGTTAAAAGTACAAATAAGTATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATATTTACAAGATCCGTTCATGAACGCACTGAG‑TCGGGAACGTTTTCAAGATTCTAATTATTTGCTGAAAGGTATTTAGGTGCAAtggaga                                                                                             >  2:1079048/1‑147 (MQ=255)

                                                           cGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAAt                                                                                           >  2:274108/1‑150 (MQ=255)

                                                             aaaGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCg                                                                                         <  1:289850/150‑1 (MQ=255)

                                                               aGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAg                                                                                       <  1:2022300/150‑1 (MQ=255)

                                                                  ttCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCt                                                                                    >  1:441419/1‑150 (MQ=255)

                                                                   tCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGCAACGAGTTCCAGTTACTATTTATTTGGTCAATGGTATTAAGCTCCAAGGGCAAATCGAGTCta                                                                                   >  1:941848/1‑149 (MQ=255)

                                                                     aaaGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCtttt                                                                                 <  1:603371/150‑1 (MQ=255)

                                                                      aaGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑ACGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGAAAATCGAGTAttttt                                                                                >  1:2239375/1‑149 (MQ=255)

                                                                        gTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGAt                                                                              >  2:324673/1‑150 (MQ=255)

                                                                           cAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAg                                                                           >  2:1280853/1‑150 (MQ=255)

                                                                           cAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATACGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGATGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAg                                                                           >  2:1515528/1‑150 (MQ=255)

                                                                               tAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCg                                                                       >  1:454104/1‑150 (MQ=255)

                                                                               tAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCg                                                                       <  2:412820/150‑1 (MQ=255)

                                                                                     tataAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATcc                                                                 <  1:364883/150‑1 (MQ=255)

                                                                                         aGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATcc                                                                 <  2:1686495/146‑1 (MQ=255)

                                                                                           gAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGa                                                           >  1:1945099/1‑150 (MQ=255)

                                                                                           gAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGa                                                           <  2:712988/150‑1 (MQ=255)

                                                                                              aaGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGaaaa                                                        <  1:1659931/150‑1 (MQ=255)

                                                                                                gagagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAac                                                      <  2:971941/150‑1 (MQ=255)

                                                                                                gagagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAac                                                      <  2:1397331/150‑1 (MQ=255)

                                                                                                  gagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAacac                                                    <  2:365201/150‑1 (MQ=255)

                                                                                                    gaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACgg                                                  <  2:916458/150‑1 (MQ=255)

                                                                                                        ggCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAg                                              >  2:554012/1‑150 (MQ=255)

                                                                                                           tAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCa                                           <  1:2453748/150‑1 (MQ=255)

                                                                                                            aaGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAg                                          <  1:1118077/150‑1 (MQ=255)

                                                                                                                ggCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATgg                                      >  1:1201907/1‑150 (MQ=255)

                                                                                                                   aaTCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGttt                                   <  1:980948/150‑1 (MQ=255)

                                                                                                                    aTCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTa                                  >  1:998368/1‑150 (MQ=255)

                                                                                                                    aTCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTa                                  <  1:2336213/150‑1 (MQ=255)

                                                                                                                      cTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACa                                <  1:2417918/150‑1 (MQ=255)

                                                                                                                      cTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACa                                <  1:9215/150‑1 (MQ=255)

                                                                                                                      cTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACa                                <  2:2138982/150‑1 (MQ=255)

                                                                                                                          aCAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCa                            >  2:2398907/1‑150 (MQ=255)

                                                                                                                           tggaacacGTTCCCG‑ACGCAGTGCGTTCAGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAc                           >  1:1131170/8‑150 (MQ=255)

                                                                                                                           cAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAc                           >  1:1508199/1‑150 (MQ=255)

                                                                                                                           cAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAAAGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTTATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTAAAAGCaa                           >  2:842182/1‑149 (MQ=255)

                                                                                                                             gaacacGTTCCCG‑ACGCAGTGCGTTCAGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcgc                         >  2:1217689/6‑150 (MQ=255)

                                                                                                                             gaacacGTTCCCG‑ACGCAGTGCGTTCAGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATACTGTTGAAAAACACGGTCAGCCAGAGGGTTTACAAGCCcgc                         >  2:2113289/6‑150 (MQ=255)

                                                                                                                              aacacGTTCCCG‑ACGCAGTGCGTTCAGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcgcg                        >  2:1659319/5‑150 (MQ=255)

                                                                                                                                tCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGAt                      >  2:262769/1‑150 (MQ=255)

                                                                                                                                  cGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGAttt                    >  1:1792961/1‑150 (MQ=255)

                                                                                                                                  cGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGAttt                    <  1:1677336/150‑1 (MQ=255)

                                                                                                                                      ccTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTAc                <  1:850724/150‑1 (MQ=255)

                                                                                                                                      ccTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTAc                >  2:657814/1‑150 (MQ=255)

                                                                                                                                       cTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACt               >  1:37067/1‑150 (MQ=255)

                                                                                                                                       cTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACt               <  2:2291091/150‑1 (MQ=255)

                                                                                                                                        tGAAGGCAGTGCG‑TCGGGAACGTGTTCCAGTTTCTTTTTATTTGGTGAATGGTATTAAGCTGGAAGGGCAAATGGAGTCTTTTGATGTGTTGGTGATCCTGTTGAAAAAGACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtg              <  2:941848/150‑1 (MQ=255)

                                                                                                                                         gAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtgt             <  1:1280853/150‑1 (MQ=255)

                                                                                                                                                  gCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGtc    >  1:1414094/1‑150 (MQ=255)

                                                                                                                                                    g‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCTTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGtc    <  2:1010810/148‑1 (MQ=255)

                                                                                                                                                    g‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCTTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGtc    >  1:1010810/1‑148 (MQ=255)

                                                                                                                                                    g‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTCCTACTGTTGTCCCGtctc  <  1:779619/150‑1 (MQ=255)

GTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCG‑TCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTC  >  NC_000913/4400186‑4400483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`