BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,609,561:1	+C	6.2%	coding (1120/1452 nt)	uxaB ←	tagaturonate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,609,561	1	.	C	6.2%	658.6 / 39.6	161	coding (1120/1452 nt)	uxaB	tagaturonate reductase
Reads supporting (aligned to +/- strand): ref base . (72/79); new base C (8/2); total (80/81)
Fisher's exact test for biased strand distribution p-value = 5.65e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

TTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAGTACCGGAATAATTT  >  NC_000913/1609412‑1609710

ttGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtg                                                                                                                                                        <  2:2145254/150‑1 (MQ=255)

 tGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtg‑t                                                                                                                                                      >  2:483903/1‑150 (MQ=255)

  gCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtg‑tg                                                                                                                                                     >  1:213694/1‑150 (MQ=255)

  gCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCCGCCCTGCCAGCAGCtg‑tg                                                                                                                                                     <  1:616931/150‑1 (MQ=255)

    tGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGc                                                                                                                                                   >  1:457763/1‑150 (MQ=255)

    tGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGc                                                                                                                                                   >  1:2162994/1‑150 (MQ=255)

      aTAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAg                                                                                                                                                 <  2:771844/150‑1 (MQ=255)

         aCGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGAt                                                                                                                                              <  2:1106838/150‑1 (MQ=255)

         aCGTTCAAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTAAGCTCACCACGATAGAACGCAATCAATGCCGCTAATCCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGAt                                                                                                                                              >  1:163519/1‑150 (MQ=255)

          cGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATg                                                                                                                                             >  1:965748/1‑150 (MQ=255)

           gTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGc                                                                                                                                            >  1:2021411/1‑150 (MQ=255)

            ttCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCg                                                                                                                                           <  1:1735428/150‑1 (MQ=255)

              ccagccagTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCATGATGCggg                                                                                                                                         <  2:2017994/150‑1 (MQ=255)

              ccagccagTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCggg                                                                                                                                         <  2:2225435/150‑1 (MQ=255)

              ccagccagTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCggg                                                                                                                                         <  2:133673/150‑1 (MQ=255)

              ccagccagTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCggg                                                                                                                                         <  2:2346127/150‑1 (MQ=255)

               cagccagTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGt                                                                                                                                        >  2:822042/1‑150 (MQ=255)

                agccagTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTATGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTg                                                                                                                                       >  2:1884840/1‑150 (MQ=255)

                  ccagTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTACTGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCg                                                                                                                                     <  1:2354121/150‑1 (MQ=255)

                  ccagTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCg                                                                                                                                     >  2:927971/1‑150 (MQ=255)

                    agTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGa                                                                                                                                   <  1:593843/150‑1 (MQ=255)

                     gtgtGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGTCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGaa                                                                                                                                  >  2:499001/1‑150 (MQ=255)

                          cATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTgg                                                                                                                             <  1:1571136/150‑1 (MQ=255)

                            tCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTc                                                                                                                           <  2:1686172/150‑1 (MQ=255)

                            tCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTc                                                                                                                           <  2:1343722/150‑1 (MQ=255)

                                 cTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGATG‑TGGCAGGATGCGGGGGCGGAACTTGGTCATAcc                                                                                                                      <  2:379544/150‑1 (MQ=255)

                                   gTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGt                                                                                                                    >  1:1537304/1‑150 (MQ=255)

                                     aCCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTg                                                                                                                  <  2:1571547/150‑1 (MQ=255)

                                        ggATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAgc                                                                                                               >  2:453478/1‑150 (MQ=255)

                                         gATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAgcg                                                                                                              <  2:3449/150‑1 (MQ=255)

                                          atatGTTTCGCCGGTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAgcgc                                                                                                             <  1:222325/150‑1 (MQ=255)

                                              gTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATa                                                                                                         <  2:1435204/150‑1 (MQ=255)

                                               tttCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAg                                                                                                        <  2:2272348/150‑1 (MQ=255)

                                                  cGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAca                                                                                                     <  1:927971/150‑1 (MQ=255)

                                                     cGTTACGCTCACCATGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagca                                                                                                  >  2:1224344/1‑150 (MQ=255)

                                                     cGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagca                                                                                                  <  1:2000467/150‑1 (MQ=255)

                                                       ttACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGCGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACagcagc                                                                                                <  2:1552002/150‑1 (MQ=255)

                                                            cTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTTGTCATACCGTTGAGCGCGATTGACAGCAGCTGATg                                                                                           <  1:348965/150‑1 (MQ=255)

                                                            cTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGGCATACCGTTGAGCGCGATAGACAGCAGCTGATg                                                                                           <  2:2490/150‑1 (MQ=255)

                                                             tCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGc                                                                                          <  1:1621939/150‑1 (MQ=255)

                                                              caccacGATAGCACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCt                                                                                         >  2:1296069/1‑150 (MQ=255)

                                                              caccacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCt                                                                                         >  2:1304436/1‑150 (MQ=255)

                                                               accacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCtt                                                                                        <  2:636493/150‑1 (MQ=255)

                                                                ccacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTa                                                                                       >  1:665563/1‑150 (MQ=255)

                                                                ccacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTa                                                                                       >  2:614817/1‑150 (MQ=255)

                                                                ccacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTa                                                                                       <  1:1284584/150‑1 (MQ=255)

                                                                  acGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAAt                                                                                     <  1:1304500/150‑1 (MQ=255)

                                                                   cGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGTTGCGGAACTTGGTCATACCGTTGAGCGCGATATACAGCAGCTGATGCTTAATg                                                                                    <  2:1565830/150‑1 (MQ=255)

                                                                   cGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGGCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATg                                                                                    <  2:1296647/150‑1 (MQ=255)

                                                                      tAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTAc                                                                                 >  1:1073397/1‑150 (MQ=255)

                                                                         aaCGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACggg                                                                              <  1:1527017/150‑1 (MQ=255)

                                                                         aaCGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACggg                                                                              >  2:1343365/1‑150 (MQ=255)

                                                                         aaCGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACggg                                                                              <  2:186922/150‑1 (MQ=255)

                                                                             caatcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTAc                                                                          >  2:236959/1‑150 (MQ=255)

                                                                               atcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCCTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACgg                                                                        >  2:164567/1‑150 (MQ=255)

                                                                                 caatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACTGGTTACGGaa                                                                      >  1:901446/1‑150 (MQ=255)

                                                                                  aatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGaaa                                                                     <  2:1242840/150‑1 (MQ=255)

                                                                                  aatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGaaa                                                                     >  2:1818567/1‑150 (MQ=255)

                                                                                   atGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAAc                                                                    >  2:2488999/1‑150 (MQ=255)

                                                                                    tGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACg                                                                   >  1:1850680/1‑150 (MQ=255)

                                                                                     gCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGc                                                                  >  1:606214/1‑150 (MQ=255)

                                                                                       cGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACTccc                                                                <  2:2162994/150‑1 (MQ=255)

                                                                                             tGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGAc                                                          >  1:1839430/1‑150 (MQ=255)

                                                                                             tGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGAc                                                          >  1:1800788/1‑150 (MQ=255)

                                                                                             tGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGAc                                                          >  2:1794772/1‑150 (MQ=255)

                                                                                             tGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCACTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGAc                                                          >  2:1467577/1‑150 (MQ=255)

                                                                                               cGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTg                                                        <  1:1916604/150‑1 (MQ=255)

                                                                                                  aaGTAAGGCGCGCCGGAAGTGTGGCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCAc                                                     <  2:2338233/150‑1 (MQ=255)

                                                                                                  aaGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCAc                                                     <  2:1840452/150‑1 (MQ=255)

                                                                                                  aaGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCAc                                                     >  1:940047/1‑150 (MQ=255)

                                                                                                       aGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCg                                                >  2:1919243/1‑150 (MQ=255)

                                                                                                       aGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCg                                                >  2:634945/1‑150 (MQ=255)

                                                                                                            cgcCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGa                                           >  2:512799/1‑150 (MQ=255)

                                                                                                                ggAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGAc                                                          <  1:2267352/131‑1 (MQ=255)

                                                                                                                ggAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTcc                                       >  2:1622567/1‑150 (MQ=255)

                                                                                                                ggAAGTGGGCCGTTTGCCTTCTGCCCTGCCAGCAGGTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTcc                                       <  2:1354271/150‑1 (MQ=255)

                                                                                                                  aaGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAg                                     <  1:1224344/150‑1 (MQ=255)

                                                                                                                    gtgtGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGtt                                   >  1:280952/1‑150 (MQ=255)

                                                                                                                       tgCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTtcaa                                >  1:538653/1‑149 (MQ=255)

                                                                                                                        gCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTtcatc                               <  1:103693/150‑1 (MQ=255)

                                                                                                                        gCCGTTTGCATTCTGACCTGCCAGCACCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTAATACCGTTGAGCGCGATAGACACCAGCTGATGCTTAATGTACGGCTAACGGAAACGCCCGCTGACTGCACACGCGAAAGATTCCAGTtcatc                               >  2:376392/1‑150 (MQ=255)

                                                                                                                         ccGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTtcatca                              >  1:2370086/1‑150 (MQ=255)

                                                                                                                          cGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCAc                             <  1:483903/150‑1 (MQ=255)

                                                                                                                                ccTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCa                       <  1:1340697/150‑1 (MQ=255)

                                                                                                                                 cccgcatcctgccA‑CAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCaa                      >  2:1930732/8‑150 (MQ=255)

                                                                                                                                 cccgcatcctgccA‑CAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCaa                      >  2:1655404/8‑150 (MQ=255)

                                                                                                                                 cccgcatcctgccA‑CAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTGAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGCTTCCAGTTCATCACGAGGCaa                      >  1:2469782/8‑150 (MQ=255)

                                                                                                                                  ccgcatcctgccA‑CAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAACCGCCCGGTCACTGCACTGGCGAAAGATTCCAGTTAATCACGAGGCaaa                     >  2:2082165/7‑150 (MQ=255)

                                                                                                                                     tgccctgccAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATcc                  <  1:757371/150‑1 (MQ=255)

                                                                                                                                     tgccctgccAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATcc                  >  1:761095/1‑150 (MQ=255)

                                                                                                                                     catcctgccA‑CAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATcc                  >  2:80431/4‑150 (MQ=255)

                                                                                                                                      gccctgccAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCa                 >  2:185570/1‑150 (MQ=255)

                                                                                                                                        cctgccAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAGt               >  1:1166848/1‑150 (MQ=255)

                                                                                                                                         ctgccAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAGTa              >  1:1957916/1‑150 (MQ=255)

                                                                                                                                            gccacagcTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAg                                            <  1:1443538/114‑1 (MQ=255)

                                                                                                                                             cagcagCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAGTACCgg          <  1:1467577/150‑1 (MQ=255)

                                                                                                                                                cagcTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAGTACCGGaat       <  1:2488999/150‑1 (MQ=255)

                                                                                                                                                  gcTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAGTACCGGaataa     <  2:940434/150‑1 (MQ=255)

                                                                                                                                                     g‑tgGCAGGATGCGGGTGCGGAACTTGGTCATACAGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAGTACCGGAATAAttt  >  1:109748/1‑150 (MQ=255)

                                                                                                                                                     g‑tgGCAGGATGCGGGTGAGGAAATTGGTCATAGAGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCAGGTGACTGAACTGGCGAACGATTCCCGTTGATCCCGAGGCAAATCCAGTACCGGAAGAAtta  >  2:1117846/1‑149 (MQ=255)

TTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAGTACCGGAATAATTT  >  NC_000913/1609412‑1609710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`