BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,906,229	T→G	6.3%	I181S (ATC→AGC) ‡	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,906,229	0	T	G	6.3%	476.6 / 19.5	159	I181S (ATC→AGC) ‡	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand): ref base T (85/64); new base G (5/5); total (90/69)
Fisher's exact test for biased strand distribution p-value = 7.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

Only 100 of 160 total aligned reads displayed.

CAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCG  >  NC_000913/1906080‑1906377

caccactATCGCGACCGCATTGGCCACTCGTGGTGCAACCTTGATTATGTCACCATTAGGGATGATGGTTGGGCGCTTTATCGGCTCAATTATTGGGAAAACAGCGGAAATTCTCGGCGGGCTGGTGCGGATCGGCATCGGCGTCCAGAt                                                                                                                                                      <  2:849812/144‑1 (MQ=255)

      tATCTCGACCGTATTGGCTATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATTGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGTAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTg                                                                                                                                                <  2:1070087/150‑1 (MQ=255)

         cgcgACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGAGGATCTGGAc                                                                                                                                             <  1:256114/150‑1 (MQ=255)

          gcgACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACg                                                                                                                                            >  2:155260/1‑150 (MQ=255)

          gcgACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGAGGATCTGGACg                                                                                                                                            <  1:1379494/150‑1 (MQ=255)

           cgACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGAGGATCTGGACGc                                                                                                                                           <  1:975209/150‑1 (MQ=255)

            gACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGAGGATCTGGACGcc                                                                                                                                          <  2:2050894/150‑2 (MQ=255)

             aCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCAc                                                                                                                                         >  2:2172924/1‑150 (MQ=255)

                    tGGCCATTGGTTTTTCATCCTTGATTATTTAAACTTTAGGTATGATTCGTGGTTGCTTTATCGGCTCTTTTTTTGTGAATATGCCGGTAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCagg                                                                                                                                  <  2:713485/150‑3 (MQ=255)

                        cATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTta                                                                                                                              <  1:1017505/150‑1 (MQ=255)

                        cATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTta                                                                                                                              >  2:1225862/1‑150 (MQ=255)

                        cATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTta                                                                                                                              >  1:1662569/1‑150 (MQ=255)

                           tGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTtaata                                                                                                                           <  1:1184116/150‑1 (MQ=255)

                             gTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAg                                                                                                                         >  1:821211/1‑150 (MQ=255)

                             gTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAg                                                                                                                         >  2:968125/1‑150 (MQ=255)

                             gTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAg                                                                                                                         <  2:1649995/150‑1 (MQ=255)

                             gTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAg                                                                                                                         <  2:677263/150‑1 (MQ=255)

                                gtgCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAAc                                                                                                                      <  1:1272147/150‑1 (MQ=255)

                                 tgCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACg                                                                                                                     >  2:1468124/1‑150 (MQ=255)

                                  gCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTTGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGc                                                                                                                    >  2:1433020/1‑150 (MQ=255)

                                  gCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGc                                                                                                                    >  2:1908169/1‑150 (MQ=255)

                                  gCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTATCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGc                                                                                                                    >  2:1342650/1‑150 (MQ=255)

                                    aaCCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTg                                                                                                                  <  1:1259521/150‑1 (MQ=255)

                                     aCCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGc                                                                                                                 >  1:1046190/1‑150 (MQ=255)

                                       cTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCTATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCa                                                                                                               >  2:1822601/1‑150 (MQ=255)

                                       cTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCa                                                                                                               >  1:623970/1‑150 (MQ=255)

                                       cTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATACTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCa                                                                                                               >  1:2095421/1‑150 (MQ=255)

                                        ttGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCag                                                                                                              <  1:699712/150‑1 (MQ=255)

                                                 tCAACATTAGGGATGATGGTTGGACGCTTTATCGGCTCAAATATTGGGAAAAAACCGGAAATTCTCGGAGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACCGTAAATAAGAACGCTGCCAGAGGTGAata                                                                                                     >  2:465356/1‑150 (MQ=255)

                                                   aaCATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATAtt                                                                                                   >  1:1530320/1‑150 (MQ=255)

                                                    aCATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTa                                                                                                  >  2:195141/1‑150 (MQ=255)

                                                       ttAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAg                                                                                               <  1:2191903/150‑1 (MQ=255)

                                                       ttAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAg                                                                                               <  2:1016367/150‑1 (MQ=255)

                                                        tAGGGATGATGGTTGGTCGCTTTATCAGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGt                                                                                              >  2:2351581/1‑150 (MQ=255)

                                                            gatgatGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGt                                                                                          >  2:2404120/1‑150 (MQ=255)

                                                              tgatgGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCt                                                                                        >  1:1453112/1‑150 (MQ=255)

                                                               gatgGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTg                                                                                       <  2:1417786/150‑1 (MQ=255)

                                                                 tggttggtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGc                                                                                     <  1:2032822/150‑1 (MQ=255)

                                                                 tggttggtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGc                                                                                     <  1:407508/150‑1 (MQ=255)

                                                                 tggttggtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGc                                                                                     <  1:1928174/150‑1 (MQ=255)

                                                                 tgggtggtCGCTTTTTCGGCTCAATTATTGGGAAAAACCCGTAAATTCTCGGCGGGGGGGTGGTTATGGGCTTCGGGGTCCATATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGc                                                                                     <  1:602832/150‑1 (MQ=255)

                                                                   gttggtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAg                                                                                   >  2:2420440/1‑150 (MQ=255)

                                                                    ttggtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGt                                                                                  <  1:155260/150‑1 (MQ=255)

                                                                      ggtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCg                                                                                <  2:1662569/150‑1 (MQ=255)

                                                                       gtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGa                                                                               >  2:206673/1‑150 (MQ=255)

                                                                        tCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGCTCGGCATCGGCGTCCAGATCCTCTGGCCGCACTTCCACGGTTAATAAGAACGCTGCCAGCGGTGAATATTAAAGTCCGTCTGGCAGTCGac                                                                              >  2:87516/1‑149 (MQ=255)

                                                                          ggtttATCGGCTCAATTATTGGGAAAAAAGCGGATATTCTCGGCGGGCTGGTGCTGATGGGCATCGGCGTCCAGATCCTCTGGACGCACTTGGTCGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAaca                                                                            <  1:465356/148‑1 (MQ=255)

                                                                          gCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTTATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAaca                                                                            <  1:474075/150‑1 (MQ=255)

                                                                           cTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAac                                                                             >  1:405729/1‑148 (MQ=255)

                                                                           cTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAac                                                                             <  2:405729/148‑1 (MQ=255)

                                                                             ttATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACActt                                                                         >  1:433007/1‑150 (MQ=255)

                                                                                 cGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCttt                                                                     <  1:546736/150‑1 (MQ=255)

                                                                                  ggCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCtttt                                                                    >  1:712264/1‑150 (MQ=255)

                                                                                  ggCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCtttt                                                                    >  1:32906/1‑150 (MQ=255)

                                                                                   ggtCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTtg                                                                   >  2:833418/3‑150 (MQ=255)

                                                                                      cAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTtgctg                                                                >  2:625808/1‑150 (MQ=255)

                                                                                          tattGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCCGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAg                                                            >  1:1023190/1‑150 (MQ=255)

                                                                                           attGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAgt                                                           >  1:136625/1‑150 (MQ=255)

                                                                                           attGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAgt                                                           >  1:1858287/1‑150 (MQ=255)

                                                                                             tGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTACGTCTGGCAGTCGAACACTTCTTTTGCTGCCAgtgt                                                         >  2:1618857/1‑150 (MQ=255)

                                                                                               ggAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGttt                                                       >  1:2472755/1‑150 (MQ=255)

                                                                                                gAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTg                                                      <  1:784420/150‑1 (MQ=255)

                                                                                                  aaaaaGCGGAAATTCTCGGAGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGcc                                                    >  2:2316871/1‑150 (MQ=255)

                                                                                                    aaaGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAg                                                  <  1:195141/150‑1 (MQ=255)

                                                                                                    aaaGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAg                                                  >  1:1800837/1‑150 (MQ=255)

                                                                                                      aGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGAc                                                <  1:335298/150‑1 (MQ=255)

                                                                                                        cGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACtt                                              >  1:2408414/1‑150 (MQ=255)

                                                                                                         ggAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCCGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTc                                             >  2:2386019/1‑150 (MQ=255)

                                                                                                              ttCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCt                                        >  1:1908651/1‑150 (MQ=255)

                                                                                                                 tcGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCTGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCg                                     <  1:1604853/150‑1 (MQ=255)

                                                                                                                 tcGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCg                                     >  2:1008216/1‑150 (MQ=255)

                                                                                                                 tcGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGAAGTCTGGcaaac                                     <  2:1329803/150‑5 (MQ=255)

                                                                                                                 tcGGCGGGCTGGTGCGGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGAAGTCTGGcaaac                                     <  2:2097819/150‑5 (MQ=255)

                                                                                                                           ggTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGc                           >  1:286778/1‑150 (MQ=255)

                                                                                                                           ggTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGc                           <  1:2104581/150‑1 (MQ=255)

                                                                                                                             tGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCaa                         >  2:1546759/1‑150 (MQ=255)

                                                                                                                               cTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAAc                       <  2:712264/150‑1 (MQ=255)

                                                                                                                                 gATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACgg                     >  2:31033/1‑150 (MQ=255)

                                                                                                                                 gATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACgg                     >  1:2258108/1‑150 (MQ=255)

                                                                                                                                 gATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACgg                     >  2:561481/1‑150 (MQ=255)

                                                                                                                                  atcggcatcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGc                    <  2:821211/150‑1 (MQ=255)

                                                                                                                                    cggcatcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGt                  >  1:1957649/1‑150 (MQ=255)

                                                                                                                                     ggcatcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAACCGGCggc                 >  1:2085897/1‑148 (MQ=255)

                                                                                                                                     ggcatcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTc                 >  2:301664/1‑150 (MQ=255)

                                                                                                                                           tgcGTCCAGAGGATCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGc           >  1:1065459/2‑150 (MQ=255)

                                                                                                                                           tgcGTCCAGAGGATCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGc           <  2:1065459/149‑1 (MQ=255)

                                                                                                                                           ggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGc           <  1:537446/150‑1 (MQ=255)

                                                                                                                                           ggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGc           >  2:1217038/1‑150 (MQ=255)

                                                                                                                                            gcGTCCAGAGGATCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCa          >  2:1923124/1‑150 (MQ=255)

                                                                                                                                            gcGTCCAGAGGATCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCa          >  1:1766113/1‑150 (MQ=255)

                                                                                                                                             cGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaa         <  2:1046190/150‑1 (MQ=255)

                                                                                                                                              gTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaat        <  1:2000776/150‑1 (MQ=255)

                                                                                                                                              gTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaat        <  2:1268301/150‑1 (MQ=255)

                                                                                                                                              gTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaat        <  2:875178/150‑1 (MQ=255)

                                                                                                                                               tCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaata       <  1:1908169/150‑1 (MQ=255)

                                                                                                                                                  aGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaataaag    >  2:1828131/1‑148 (MQ=255)

                                                                                                                                                  aGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATg    >  1:2467175/1‑150 (MQ=255)

                                                                                                                                                   gATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGc   <  1:910909/150‑1 (MQ=255)

                                                                                                                                                    aTCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCg  <  2:2124457/150‑1 (MQ=255)

                                                                                                                                                    aTCCTCTGGACGCACTTCCACGGTTAAAAAGAACGCTGCCAGAGGTGAATATTAAATTCCGTATGGCAGTCGAACAATTCTTTTCCTGCCAGTGTATGACAGACTTATGGCTTCGCACTCCAGGCAAACGCCGTCATATGGAATAATGcc  >  1:2484987/1‑149 (MQ=255)

CAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCG  >  NC_000913/1906080‑1906377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`