BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	192,085	C→A	9.5%	D77E (GAC→GAA)	pyrH →	UMP kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	192,085	0	C	A	9.5%	369.0 / 18.5	116	D77E (GAC→GAA)	pyrH	UMP kinase
Reads supporting (aligned to +/- strand): ref base C (59/46); new base A (5/6); total (64/52)
Fisher's exact test for biased strand distribution p-value = 5.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

Only 100 of 119 total aligned reads displayed.

GGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTG  >  NC_000913/191936‑192229

gggaTTGATGCAAGCATACTGGATCGTATGGCGCAGGAAATCAAAGAACTGGTGGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGAc                                                                                                                                                  <  2:1823045/147‑1 (MQ=255)

 gTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGAcc                                                                                                                                                 <  2:802915/150‑1 (MQ=255)

 gTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGAcc                                                                                                                                                 <  1:1663933/150‑1 (MQ=255)

   aTTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGCGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACcac                                                                                                                                               >  2:1744769/1‑150 (MQ=255)

    ttGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACcaca                                                                                                                                              >  1:1285173/1‑150 (MQ=255)

    ttGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACcaca                                                                                                                                              <  1:889775/150‑1 (MQ=255)

     tGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACAt                                                                                                                                             <  2:2039305/150‑1 (MQ=255)

        tGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGATCTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGGCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATggg                                                                                                                                          <  1:2383675/150‑1 (MQ=255)

        tGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATggg                                                                                                                                          <  1:1259333/150‑1 (MQ=255)

        tGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATggg                                                                                                                                          <  1:2374995/150‑1 (MQ=255)

        tGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGGCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATggg                                                                                                                                          <  1:945644/150‑1 (MQ=255)

            aGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATg                                                                                                                                      >  1:296843/1‑150 (MQ=255)

                 aCTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGGCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGc                                                                                                                                 <  1:1109983/150‑1 (MQ=255)

                  cTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCg                                                                                                                                <  2:1684256/150‑1 (MQ=255)

                  cTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCg                                                                                                                                >  1:890583/1‑150 (MQ=255)

                    ggATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGTTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGAc                                                                                                                              <  2:654457/150‑1 (MQ=255)

                    ggATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGAc                                                                                                                              >  2:530087/1‑150 (MQ=255)

                         gTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTaa                                                                                                                         >  2:2316417/1‑150 (MQ=255)

                          tATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAAt                                                                                                                        <  2:1782916/150‑1 (MQ=255)

                          tATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAAt                                                                                                                        <  1:41063/150‑1 (MQ=255)

                                   ggAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCt                                                                                                               >  1:2182595/1‑150 (MQ=255)

                                      aaTCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGc                                                                                                            >  1:2187877/1‑150 (MQ=255)

                                      aaTCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGc                                                                                                            <  1:762286/150‑1 (MQ=255)

                                       aTCAAAGATCTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCa                                                                                                           <  2:141894/150‑1 (MQ=255)

                                       aTCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGGCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCa                                                                                                           <  2:2474434/150‑1 (MQ=255)

                                        tCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCaa                                                                                                          >  1:1590050/1‑150 (MQ=255)

                                        tCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACATGTTCCGTGGCGCTGGTATGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGATGCCGCCCGTAATGAACGGCATGGCaa                                                                                                          >  2:1066033/1‑150 (MQ=255)

                                         cAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAAt                                                                                                         <  1:1748310/150‑1 (MQ=255)

                                         cAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAAt                                                                                                         >  1:1470549/1‑150 (MQ=255)

                                          aaaGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATg                                                                                                        <  1:941414/150‑1 (MQ=255)

                                           aaGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGc                                                                                                       >  1:2287175/1‑150 (MQ=255)

                                           aaGAACTGGTGGAACTGGGTATTCAGGGTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGAACACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGc                                                                                                       <  2:1630973/150‑1 (MQ=255)

                                            aGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCg                                                                                                      >  2:521951/1‑150 (MQ=255)

                                             gAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGAACACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGt                                                                                                     >  2:269190/1‑150 (MQ=255)

                                              aaCTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTg                                                                                                    <  1:323634/150‑1 (MQ=255)

                                               aCTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGa                                                                                                   <  2:601456/150‑1 (MQ=255)

                                                 tGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGAtg                                                                                                 <  2:1315343/150‑1 (MQ=255)

                                                       aaCTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGAtgcactgc                                                                                           <  2:1052830/150‑1 (MQ=255)

                                                       aaCTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGAtgcactgc                                                                                           <  2:639374/150‑1 (MQ=255)

                                                       aaCTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGAACACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGAtgcactgc                                                                                           <  1:1205371/150‑1 (MQ=255)

                                                               aTTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGcc                                                                                   <  1:490514/150‑1 (MQ=255)

                                                                 tCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTa                                                                                 >  2:1301493/1‑150 (MQ=255)

                                                                 tCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTa                                                                                 >  2:960844/1‑150 (MQ=255)

                                                                  cAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTAt                                                                                >  1:1913543/1‑150 (MQ=255)

                                                                   aGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTAtg                                                                               <  1:657648/150‑1 (MQ=255)

                                                                    ggTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTAtgt                                                                              >  1:279059/1‑150 (MQ=255)

                                                                     gTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGAACACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTAtgtg                                                                             <  2:1420530/150‑1 (MQ=255)

                                                                        ggtgtggtgATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAAc                                                                          >  1:1686309/1‑150 (MQ=255)

                                                                        ggtgtggtgATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAAc                                                                          <  1:1301493/150‑1 (MQ=255)

                                                                          tgtggtgATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTAAGTGAACGc                                                                        >  1:1079307/1‑150 (MQ=255)

                                                                             ggtgATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCg                                                                     >  2:565899/1‑150 (MQ=255)

                                                                             ggtgATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGAACACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCg                                                                     <  1:269190/150‑1 (MQ=255)

                                                                                  ttGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGa                                                                <  1:578009/150‑1 (MQ=255)

                                                                                  ttGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGa                                                                <  1:590692/150‑1 (MQ=255)

                                                                                    ggtggGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATg                                                              >  1:2480173/1‑150 (MQ=255)

                                                                                          ggTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCt                                                        >  2:1107643/1‑150 (MQ=255)

                                                                                                cTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACAGGGGGATGCTGGCGACCGTAATGAACGGCCTGGAAATGCGTGATGCACTGCACCGCGACTATGTGAACGCTAGTCTGATGTCCGCTATTCCa                                                  >  1:1574459/1‑150 (MQ=255)

                                                                                                 tGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCAt                                                 <  2:1145056/150‑1 (MQ=255)

                                                                                                 tGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCAt                                                 >  2:1893081/1‑150 (MQ=255)

                                                                                                  gTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATTAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCAtt                                                >  2:738814/1‑150 (MQ=255)

                                                                                                  gTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCGAtt                                                >  2:1678336/1‑150 (MQ=255)

                                                                                                  gTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCAtt                                                >  2:54925/1‑150 (MQ=255)

                                                                                                  gTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCAtt                                                >  2:501580/1‑150 (MQ=255)

                                                                                                  gTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCAtt                                                >  1:1670084/1‑150 (MQ=255)

                                                                                                  gTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCAtt                                                >  1:1088682/1‑150 (MQ=255)

                                                                                                   ttCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTTGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTg                                               <  2:1691697/150‑1 (MQ=255)

                                                                                                      cGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAAt                                            <  2:1590050/150‑1 (MQ=255)

                                                                                                            ccTGGTCTGGCGTAAGCGGGTATGAACCGCGTGGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTACACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCgtg                                      <  2:1559312/149‑1 (MQ=255)

                                                                                                             cTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCTACCGTAATGAACGGCATGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCgtgt                                     <  2:1559473/150‑1 (MQ=255)

                                                                                                               ggTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGc                                   >  1:2197067/1‑150 (MQ=255)

                                                                                                                gTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCg                                  >  1:2466292/1‑150 (MQ=255)

                                                                                                                  cTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGac                                >  1:863639/1‑150 (MQ=255)

                                                                                                                   tGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGaca                               >  2:954367/1‑150 (MQ=255)

                                                                                                                   tGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGaca                               <  1:434657/150‑1 (MQ=255)

                                                                                                                    ggCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGacag                              >  1:664324/1‑150 (MQ=255)

                                                                                                                    ggCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGacag                              >  2:1574138/1‑150 (MQ=255)

                                                                                                                    ggCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGacag                              >  1:1154165/1‑150 (MQ=255)

                                                                                                                     gCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGacagc                             >  1:1872582/1‑150 (MQ=255)

                                                                                                                      cGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGacagct                            >  1:1633162/1‑150 (MQ=255)

                                                                                                                        aaaGCGGGTATGAACCGCGTTGTGGGCGAACACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGacagctac                          <  2:1132278/150‑1 (MQ=255)

                                                                                                                         aaGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGacagctaca                         >  2:2050432/1‑150 (MQ=255)

                                                                                                                          aGCGGGTATGAACCGCGTTGTGGGCGAACACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGCCGGGGGCGccagctaccg                        >  1:161368/1‑148 (MQ=255)

                                                                                                                            cGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGacagctacagct                      >  2:866764/1‑150 (MQ=255)

                                                                                                                            cGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGacagctacagct                      >  1:806892/1‑150 (MQ=255)

                                                                                                                            cGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGacagctacagct                      >  1:133488/1‑150 (MQ=255)

                                                                                                                               gTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTggg                   <  2:635680/150‑1 (MQ=255)

                                                                                                                                tATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGGATGGCGTGTGCGACAGCTACAGCTGGGc                  <  2:444997/150‑1 (MQ=255)

                                                                                                                                   gAACCGCGTTGTGGGCGAACACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGa               >  1:959265/1‑150 (MQ=255)

                                                                                                                                    aaCCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGaa              >  1:1103489/1‑150 (MQ=255)

                                                                                                                                     aCCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAg             <  2:1286558/150‑1 (MQ=255)

                                                                                                                                     aCCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAg             >  2:1875814/1‑150 (MQ=255)

                                                                                                                                     aCCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAg             <  1:530087/150‑1 (MQ=255)

                                                                                                                                      ccGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGc            <  1:2316417/150‑1 (MQ=255)

                                                                                                                                       cgcgTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCt           >  2:63705/1‑150 (MQ=255)

                                                                                                                                        gcgGTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGACCGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGCCCGCTATTCCATTGAATGGCGTGGGCGACAGCTACAGCTGGGCAGAAGCTa          <  2:1045712/150‑1 (MQ=255)

                                                                                                                                            tgtgGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAg      <  1:1574138/150‑1 (MQ=255)

                                                                                                                                            tgtgGGCGAACACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAg      >  2:296954/1‑150 (MQ=255)

                                                                                                                                             gtgGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACAGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGc     >  2:2414317/1‑150 (MQ=255)

                                                                                                                                              tgGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGcc    <  1:2173361/150‑1 (MQ=255)

                                                                                                                                                ggCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTg  >  1:1704536/1‑150 (MQ=255)

GGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTG  >  NC_000913/191936‑192229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`