Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,205,656 A→G 5.5% Y354C (TAT→TGT) ‡ yehL → putative AAA(+) MoxR family ATPase YehL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,205,6560AG 5.5% 450.3 / 11.1 146Y354C (TAT→TGT) ‡yehLputative AAA(+) MoxR family ATPase YehL
Reads supporting (aligned to +/- strand):  ref base A (71/67);  new base G (2/6);  total (73/73)
Fisher's exact test for biased strand distribution p-value = 2.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

Only 100 of 153 total aligned reads displayed.

TTTTTAGCGAATCGCGCGGGGGAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCC  >  NC_000913/2205508‑2205803
                                                                                                                                                    |                                                                                                                                                   
tttttAGCGAATCGCGCGGGGGAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCttat                                                                                                                                                    >  1:566511/1‑150 (MQ=255)
tttttAGCGAATCGCGCGGGGGAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCttat                                                                                                                                                    >  1:1116996/1‑150 (MQ=255)
 ttttAGCGAATCGCGCGGGGGAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCAGTGGCAGGCttatt                                                                                                                                                   <  2:1539834/150‑1 (MQ=255)
  tttAGCGAATCGCGCGGGGGAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCttatta                                                                                                                                                  <  1:124906/150‑1 (MQ=255)
   ttAGCGAATCGCGCGGGGGAGCCTGCCGACCTGGTGGAATGTATTTCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTtattat                                                                                                                                                 >  2:1012049/1‑150 (MQ=255)
    tAGCGAATCGCGCGGGGGAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATc                                                                                                                                                <  1:1375894/150‑1 (MQ=255)
     aGCGAATCGCGCGGGGGAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCa                                                                                                                                               <  1:1966637/150‑1 (MQ=255)
     aGCGAATCGCGCGGGGGAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGGCTGCTCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCa                                                                                                                                               <  1:2379923/150‑1 (MQ=255)
            cgcgcgGGGGAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATc                                                                                                                                                >  1:1364341/1‑142 (MQ=255)
             gcgcgGGGGAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCGGGCTTGATAATAAGCCTGcc                                                                                                                                       <  1:500407/150‑1 (MQ=255)
             gcgcgGGGGAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGcc                                                                                                                                       >  2:2373740/1‑150 (MQ=255)
               gcgGGGGAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGGCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCGGGCTTGATAATAAGCCTGCCag                                                                                                                                     <  2:2290871/150‑2 (MQ=255)
                   gggAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCGGGCTTGATAATAAGCCTGCCagtgag                                                                                                                                 <  2:1404176/150‑6 (MQ=255)
                   gggAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCGGGCTTGATAATAAGCCTGCCagtgag                                                                                                                                 <  2:2369954/150‑6 (MQ=255)
                     gAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTg                                                                                                                               <  2:934509/150‑1 (MQ=255)
                     gAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTg                                                                                                                               <  2:2417850/150‑1 (MQ=255)
                       gCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGcc                                                                                                                             >  1:1817652/1‑150 (MQ=255)
                        ccTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCg                                                                                                                            <  1:1104947/150‑1 (MQ=255)
                         cTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGt                                                                                                                           >  2:1853409/1‑150 (MQ=255)
                          tGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTg                                                                                                                          <  1:1601090/150‑1 (MQ=255)
                           gCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGa                                                                                                                         >  2:1669548/1‑150 (MQ=255)
                            ccGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAg                                                                                                                        <  1:1303724/150‑1 (MQ=255)
                             cGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAgg                                                                                                                       <  2:411697/150‑1 (MQ=255)
                               aCCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGaa                                                                                                                     >  2:2134989/1‑150 (MQ=255)
                                  tggtggAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGCTCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAgc                                                                                                                  >  2:1422962/1‑149 (MQ=255)
                                   ggtggAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGAt                                                                                                                 <  2:197409/150‑1 (MQ=255)
                                   ggtggAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGAt                                                                                                                 >  2:1605565/1‑150 (MQ=255)
                                    gtggAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATg                                                                                                                >  1:516231/1‑150 (MQ=255)
                                    gtggAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATg                                                                                                                <  2:1114100/150‑1 (MQ=255)
                                     tggAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGc                                                                                                               >  1:985997/1‑150 (MQ=255)
                                      ggAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCGAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCa                                                                                                              >  2:576644/1‑150 (MQ=255)
                                        aaTGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATg                                                                                                            >  2:2488246/1‑150 (MQ=255)
                                           gTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATgagc                                                                                                         <  1:543508/150‑1 (MQ=255)
                                            tATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATgagcg                                                                                                        >  1:2291110/1‑150 (MQ=255)
                                             aTTGCCGGAACCATCGTCAAAGATAACTAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGa                                                                                                                  <  2:537544/139‑1 (MQ=255)
                                             aTTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGa                                                                                                                  >  1:537544/1‑139 (MQ=255)
                                                gCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGcc                                                                                                    >  2:2203831/1‑150 (MQ=255)
                                                gCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGcc                                                                                                    >  2:331648/1‑150 (MQ=255)
                                                gCCGGAACCATCGACAAAGATAACGAACAAGATCGCGCGAGTCTGAGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTCAGGAAAGATGCATGAGAGAGcc                                                                                                    >  2:82853/1‑150 (MQ=255)
                                                 ccGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCg                                                                                                   <  1:1384622/150‑1 (MQ=255)
                                                    gAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTa                                                                                                <  1:93412/150‑1 (MQ=255)
                                                     aaCCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTaa                                                                                               <  1:801392/150‑1 (MQ=255)
                                                     aaCCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTaa                                                                                               <  1:184867/150‑1 (MQ=255)
                                                      aCCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAAt                                                                                              <  1:32040/150‑1 (MQ=255)
                                                        cATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGcc                                                                                                    <  1:1436032/142‑1 (MQ=255)
                                                           cGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTcg                                                                                         <  1:1026715/150‑1 (MQ=255)
                                                           cGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTcg                                                                                         <  1:1161169/150‑1 (MQ=255)
                                                            gTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTcgg                                                                                        <  1:135751/150‑1 (MQ=255)
                                                            gTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTcgg                                                                                        <  1:2371124/150‑1 (MQ=255)
                                                            gTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTcgg                                                                                        >  1:991181/1‑150 (MQ=255)
                                                                   aTAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTcggcatccgg                                                                                 <  2:1673638/150‑1 (MQ=255)
                                                                   aTAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTcggcatccgg                                                                                 >  1:189355/1‑150 (MQ=255)
                                                                     aaCGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTcggcatccggca                                                                               <  2:255934/150‑1 (MQ=255)
                                                                      aCGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTcggcatccggcat                                                                              >  1:1958951/1‑150 (MQ=255)
                                                                       cGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTcggcatccggcatc                                                                             >  1:1732623/1‑150 (MQ=255)
                                                                       cGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTcggcatccggcatc                                                                             <  1:749722/150‑1 (MQ=255)
                                                                             agaTCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTc                                                                       <  2:124705/150‑1 (MQ=255)
                                                                                  gcgcgcgTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGcc                                                                  >  1:2145043/1‑150 (MQ=255)
                                                                                     cgcgTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTgc                                                               >  1:1861470/1‑150 (MQ=255)
                                                                                           tGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCgg                                                         <  2:527084/150‑1 (MQ=255)
                                                                                            gcgcCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGc                                                        >  2:1151670/1‑150 (MQ=255)
                                                                                             cgcCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCCCCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCt                                                       >  2:2297081/1‑150 (MQ=255)
                                                                                             cgcCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCt                                                       <  2:217016/150‑1 (MQ=255)
                                                                                                  ttACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGa                                                  <  1:2383376/150‑1 (MQ=255)
                                                                                                    aCTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGaaa                                                >  1:2282680/1‑150 (MQ=255)
                                                                                                     cTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGTCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAAt                                               <  2:562065/150‑1 (MQ=255)
                                                                                                     cTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAAt                                               >  1:1899875/1‑150 (MQ=255)
                                                                                                     cTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAAt                                               >  1:412181/1‑150 (MQ=255)
                                                                                                       tCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCg                                             >  1:1270444/1‑150 (MQ=255)
                                                                                                         gAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGTATGATAGAGCATTTAATTGTCGGCATACGTAATCATATTCAGTCCTTCTCCCTGCTGGTGTAATCGtt                                           <  2:865732/150‑1 (MQ=255)
                                                                                                          aaCAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTa                                          <  1:165941/150‑1 (MQ=255)
                                                                                                          aaCAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTa                                          <  2:291155/150‑1 (MQ=255)
                                                                                                            cAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAAt                                        >  1:589534/1‑150 (MQ=255)
                                                                                                             aGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATc                                       <  1:1605565/150‑1 (MQ=255)
                                                                                                              gCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCg                                      >  2:1524947/1‑150 (MQ=255)
                                                                                                                   tCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGc                                 <  2:1876798/150‑1 (MQ=255)
                                                                                                                   tCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGc                                 <  1:2488246/150‑1 (MQ=255)
                                                                                                                    cGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGcc                                >  1:1182444/1‑150 (MQ=255)
                                                                                                                    cGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGcc                                >  1:388433/1‑150 (MQ=255)
                                                                                                                    cGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGcc                                <  1:1012049/150‑1 (MQ=255)
                                                                                                                    cGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGcc                                >  1:2487469/1‑150 (MQ=255)
                                                                                                                       cacacaTAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGc                             >  2:769816/1‑150 (MQ=255)
                                                                                                                        acacaTAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCg                            <  1:1468477/150‑1 (MQ=255)
                                                                                                                          acaTAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGc                          >  2:1153244/1‑150 (MQ=255)
                                                                                                                          acaTAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGc                          >  2:426061/1‑150 (MQ=255)
                                                                                                                             tAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTAAATCGAAAGCCAGCGGCCAc                       >  1:1823657/1‑150 (MQ=255)
                                                                                                                              aaaGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACg                      <  1:214189/150‑1 (MQ=255)
                                                                                                                              aaaGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGAGAGCAAAGATCCATGACCGCGCCGATAATTGACGCCATCCGGCATCATACTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGCAAGCCAGCGCCCAcc                      >  2:1654955/1‑149 (MQ=255)
                                                                                                                                agaagCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGAt                    >  2:1442633/1‑150 (MQ=255)
                                                                                                                                      tCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCCGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACgtgt              >  1:323359/1‑150 (MQ=255)
                                                                                                                                       cACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGtt             <  1:1393951/150‑1 (MQ=255)
                                                                                                                                         gtGGCGGGCTTGATAATAAGCCTGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGttga           >  2:1294140/2‑150 (MQ=255)
                                                                                                                                         cTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGttga           <  1:733988/150‑1 (MQ=255)
                                                                                                                                          tGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGttgat          <  1:1304961/150‑1 (MQ=255)
                                                                                                                                           ggCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGttgatt         >  2:1915809/1‑150 (MQ=255)
                                                                                                                                              aGGCTTATTATCAAGCCCGCCACCGCCTGCCATGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGaa      <  2:1825762/150‑1 (MQ=255)
                                                                                                                                               ggCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAg     >  2:1001985/1‑150 (MQ=255)
                                                                                                                                               ggCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAg     <  2:701809/150‑1 (MQ=255)
                                                                                                                                                 cTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGc   >  2:2131089/1‑150 (MQ=255)
                                                                                                                                                  ttattaTCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGAATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGcc  >  2:1963066/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTTTTAGCGAATCGCGCGGGGGAGCCTGCCGACCTGGTGGAATGTATTGCCGGAACCATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCC  >  NC_000913/2205508‑2205803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑