BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,268,134	C→G	5.1%	L102L (CTC→CTG) ‡	yeiR →	zinc‑binding GTPase YeiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,268,134	0	C	G	5.1%	518.9 / 11.8	151	L102L (CTC→CTG) ‡	yeiR	zinc‑binding GTPase YeiR
Reads supporting (aligned to +/- strand): ref base C (82/60); new base G (5/3); total (88/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

GGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTCGCCA  >  NC_000913/2267985‑2268282

  cgcATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGa                                                                                                                                                    <  2:2431574/150‑1 (MQ=255)

   gcATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCTTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGAt                                                                                                                                                   <  1:2066075/150‑1 (MQ=255)

       tGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGt                                                                                                                                               >  2:278342/1‑150 (MQ=255)

        gCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGtt                                                                                                                                              >  1:19724/1‑150 (MQ=255)

         cTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTa                                                                                                                                             <  1:1113644/150‑1 (MQ=255)

          tGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCGAGGATCTGtttc                                                                                                                                            <  2:396849/150‑3 (MQ=255)

           gAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCGATGCAGGTAGTGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCGAGGATCTGtttcg                                                                                                                                           <  2:2147737/150‑4 (MQ=255)

           gAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGGCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAAc                                                                                                                                           <  1:2488406/150‑1 (MQ=255)

                agaTCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCAc                                                                                                                                      >  1:1169389/1‑150 (MQ=255)

                 gaTCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTCTTAACCGCAcc                                                                                                                                     >  2:2306298/1‑150 (MQ=255)

                 gaTCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAAATCCTCGATCTGCTAACCGCAcc                                                                                                                                     <  2:822557/150‑1 (MQ=255)

                     cccGGCGGCTGCATGTGCTGCGTTAATGGTTTATCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTc                                                                                                                                 <  1:156338/150‑1 (MQ=255)

                      ccGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCt                                                                                                                                >  2:1492474/1‑150 (MQ=255)

                       cggcggCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTa                                                                                                                               <  2:840313/150‑1 (MQ=255)

                            gcTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAAc                                                                                                                          >  1:2389204/1‑150 (MQ=255)

                               gCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGt                                                                                                                       >  2:1802505/1‑150 (MQ=255)

                                cATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTg                                                                                                                      <  1:1221401/150‑1 (MQ=255)

                                  tgtgCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGa                                                                                                                    <  1:1049369/150‑1 (MQ=255)

                                   gtgCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGAt                                                                                                                   >  2:2024653/1‑150 (MQ=255)

                                   gtgCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGAt                                                                                                                   >  1:714197/1‑150 (MQ=255)

                                    ggcggcGTTAATGGTTTACCCATGAAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATa                                                                                                                  <  2:1621279/146‑1 (MQ=255)

                                     gctgcGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAg                                                                                                                 >  1:829182/1‑150 (MQ=255)

                                     gctgcGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAg                                                                                                                 >  1:1963860/1‑150 (MQ=255)

                                     gctgcGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAg                                                                                                                 >  1:1244622/1‑150 (MQ=255)

                                        gcGTTAATGGTTTACTCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATc                                                                                                              <  1:1833056/150‑1 (MQ=255)

                                        gcGTTAATGGTTTACCCATGCATGTAGGGTTGAATACCTTACTGCGTCAGTGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGTGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATc                                                                                                              <  2:514452/150‑1 (MQ=255)

                                              aTGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTgcgcc                                                                                                        >  1:2432878/1‑149 (MQ=255)

                                                ggTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGcc                                                                                                      >  2:1582276/1‑150 (MQ=255)

                                                ggTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGcc                                                                                                      >  2:480653/1‑150 (MQ=255)

                                                    tACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACTGTGGATAGATCTGCGCGCCACCt                                                                                                  >  2:566273/1‑150 (MQ=255)

                                                        cATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTtgtg                                                                                              >  1:1944803/1‑150 (MQ=255)

                                                        cATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTtgtg                                                                                              >  2:1928926/1‑150 (MQ=255)

                                                            cAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTTCGCGCCAACTTGTGCAtt                                                                                          >  1:1801572/1‑150 (MQ=255)

                                                            cAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCAtt                                                                                          >  2:908345/1‑150 (MQ=255)

                                                             aGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATtc                                                                                         >  1:548720/1‑150 (MQ=255)

                                                               gTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATtctc                                                                                       <  2:2211609/150‑1 (MQ=255)

                                                                   ggTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATc                                                                                   >  1:1941211/1‑150 (MQ=255)

                                                                   ggTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATc                                                                                   <  1:278342/150‑1 (MQ=255)

                                                                          tACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCCATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTCTGAATTCTCGATCCGCGCtt                                                                            >  2:417116/1‑150 (MQ=255)

                                                                              ttACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTtgctg                                                                        >  1:2172737/1‑150 (MQ=255)

                                                                                 cTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGctgctg                                                                     >  2:1475725/1‑150 (MQ=255)

                                                                                 cTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGctgctg                                                                     <  2:1441755/150‑1 (MQ=255)

                                                                                   gCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGa                                                                   <  2:1511215/150‑1 (MQ=255)

                                                                                   gCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGa                                                                   >  1:970148/1‑150 (MQ=255)

                                                                                        aGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGaaa                                                              >  1:1151291/1‑150 (MQ=255)

                                                                                         gggAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGaaaa                                                             <  1:1343234/150‑1 (MQ=255)

                                                                                            aaaaCCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAg                                                          >  1:819878/1‑150 (MQ=255)

                                                                                                ccAGACCGCTTGTTGATAGAGCCGCCCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCGACCTTGTGCATTGTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGcc                                                      >  2:545458/1‑150 (MQ=255)

                                                                                                ccAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGcc                                                      >  2:1363182/1‑150 (MQ=255)

                                                                                                 cAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCa                                                     >  1:625266/1‑150 (MQ=255)

                                                                                                   gACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGc                                                   <  1:1492474/150‑1 (MQ=255)

                                                                                                   gACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGc                                                   <  1:325841/150‑1 (MQ=255)

                                                                                                    aCCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCa                                                  >  1:2235266/1‑150 (MQ=255)

                                                                                                    aCCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCa                                                  <  2:114406/150‑1 (MQ=255)

                                                                                                     ccGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCaa                                                 >  2:244452/1‑150 (MQ=255)

                                                                                                        cTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTtgct                                                                         <  2:1662935/123‑1 (MQ=255)

                                                                                                        cTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTtgct                                                                         >  1:1662935/1‑123 (MQ=255)

                                                                                                        cTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGa                                              >  1:1435059/1‑150 (MQ=255)

                                                                                                        cTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGa                                              >  1:1891431/1‑150 (MQ=255)

                                                                                                        cTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGAGCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGa                                              <  2:1008789/150‑1 (MQ=255)

                                                                                                         ttgttgATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGaa                                             <  2:1515124/150‑1 (MQ=255)

                                                                                                          tgttgATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGaaa                                            >  2:1146939/1‑150 (MQ=255)

                                                                                                          tgttgATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGaaa                                            >  2:794591/1‑150 (MQ=255)

                                                                                                             tgATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACt                                         >  2:2052459/1‑150 (MQ=255)

                                                                                                               aTAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTc                                       >  2:1644627/1‑150 (MQ=255)

                                                                                                                 agagCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCg                                     <  1:681741/150‑1 (MQ=255)

                                                                                                                 agagCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCg                                     <  1:2024653/150‑1 (MQ=255)

                                                                                                                   agCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTg                                   <  2:19724/150‑1 (MQ=255)

                                                                                                                     ccGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTTAc                                 <  2:2390707/150‑1 (MQ=255)

                                                                                                                      cGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGAcc                                <  1:1039038/150‑1 (MQ=255)

                                                                                                                      cGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGAcc                                <  1:2345457/150‑1 (MQ=255)

                                                                                                                       gACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCa                               >  1:470493/1‑150 (MQ=255)

                                                                                                                        aCCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAg                              <  1:122170/150‑1 (MQ=255)

                                                                                                                         ccGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATtctc                                                                                       >  2:1434675/1‑92 (MQ=255)

                                                                                                                         ccGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATtctc                                                                                       <  1:1434675/92‑1 (MQ=255)

                                                                                                                         ccGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAgc                             >  2:790925/1‑150 (MQ=255)

                                                                                                                         ccGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAgc                             >  1:2236281/1‑150 (MQ=255)

                                                                                                                          cGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAgct                            <  2:625266/150‑1 (MQ=255)

                                                                                                                           gggCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAgctg                           >  2:696105/1‑150 (MQ=255)

                                                                                                                            ggCTGGGCCATCAGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAgctgg                          <  2:427117/150‑1 (MQ=255)

                                                                                                                               tGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAgctggctg                       <  2:369957/150‑1 (MQ=255)

                                                                                                                                gggCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGc                      <  2:1944803/150‑1 (MQ=255)

                                                                                                                                  gCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACTGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCg                    <  1:566273/150‑1 (MQ=255)

                                                                                                                                   ccATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGc                   >  1:39718/1‑150 (MQ=255)

                                                                                                                                     aTCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAg                 >  2:131858/1‑150 (MQ=255)

                                                                                                                                      tCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGa                >  2:1034031/1‑150 (MQ=255)

                                                                                                                                       ccGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGAc               <  2:277508/150‑1 (MQ=255)

                                                                                                                                        gttaaCAGATCGAGGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGAca              >  1:2392618/4‑150 (MQ=255)

                                                                                                                                        gttaaCAGATCGAGGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGAca              >  2:36957/4‑150 (MQ=255)

                                                                                                                                        cGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGAca              <  1:1333033/150‑1 (MQ=255)

                                                                                                                                         ttaaCAGATCGAGGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGAcat             >  1:1152801/3‑150 (MQ=255)

                                                                                                                                          taaCAGATCGAGGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGAcatc            >  1:866327/2‑150 (MQ=255)

                                                                                                                                            aCAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGAcatcat          >  2:780803/1‑150 (MQ=255)

                                                                                                                                             cAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCAtt         >  1:209455/1‑150 (MQ=255)

                                                                                                                                               gATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGt       >  1:921700/1‑150 (MQ=255)

                                                                                                                                               gATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGt       >  1:2172408/1‑150 (MQ=255)

                                                                                                                                               gATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGt       >  1:1071689/1‑150 (MQ=255)

                                                                                                                                                aTCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTc      >  1:723048/1‑150 (MQ=255)

                                                                                                                                                   cTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTCGcc   <  1:272072/150‑1 (MQ=255)

                                                                                                                                                   cTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTCGcc   <  2:2023559/150‑1 (MQ=255)

GGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTATGAACCGTGGATAGATCTGCGCGCCACCTTGTGCATTCTCGATCCGCGCTTGCTGCTGGACGAAAAAAGCGCCAGCAATGAAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTCGCCA  >  NC_000913/2267985‑2268282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`